A5101804951- Growth Hormone and Insulin-like Growth Factors
- Hearing, Cochlea, Tinnitus, Genetics
- Biochemical Analysis and Sensing Techniques
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Hedgehog Signaling Pathway Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Vestibular and auditory disorders
- NF-κB Signaling Pathways
- Genetic Syndromes and Imprinting
- Regulation of Appetite and Obesity
- Adipokines, Inflammation, and Metabolic Diseases
- Autophagy in Disease and Therapy
- Advanced Glycation End Products research
- Phytochemistry and Bioactivity Studies
- Circular RNAs in diseases
- Neuroscience and Neuropharmacology Research
- Seaweed-derived Bioactive Compounds
- Cancer, Hypoxia, and Metabolism
- Pluripotent Stem Cells Research
- Sexual Differentiation and Disorders
- RNA Research and Splicing
- Pituitary Gland Disorders and Treatments
- Cancer-related gene regulation
- Ion channel regulation and function
Shanghai Children's Medical Center
2023-2024
Shanghai Jiao Tong University
2023-2024
Zhejiang University of Technology
2021-2024
Zhongnan Hospital of Wuhan University
2019-2024
Wuhan University
2019-2024
Hangzhou Normal University
2024
Zhejiang Chinese Medical University
2023
Universidade de São Paulo
2023
University of Michigan–Ann Arbor
2008-2022
Hangzhou Academy of Agricultural Sciences
2021
Scavenger receptor BI (SR-BI) is a cell surface that binds high density lipoproteins (HDL) and mediates selective uptake of HDL cholesteryl esters (CE) in transfected cells. To address the physiological role SR-BI cholesterol homeostasis, mice were generated bearing an promoter mutation resulted decreased expression homozygous mutant (designated att) mice. Hepatic was reduced by 53% with corresponding increase total plasma levels 50–70% att mice, attributable almost exclusively to elevated...
Protective effects of icariin on streptozotocin-induced diabetic mice by inhibiting the TLR4/NF-κB signal pathway.
Hundreds of new mutant mouse lines are being produced annually using gene targeting and trap approaches in embryonic stem (ES) cells, the number is expected to continue grow as human genome projects progress. The availability robust ES cell a simple technology for making chimeras more attractive now than ever before. We established several from 129/SvEv C57BL/6 mice tested their ability contribute germline following blastocyst injections and/or less expensive easier method morula-ES...
The precise assembly of inner ear hair cell stereocilia into rows increasing height is critical for mechanotransduction and the sense hearing. Yet, how lengths actin-based are regulated remains poorly understood. Mutations molecular motor myosin 15 stunt growth cause deafness. We found that cells express two isoforms differ by inclusion an 133-kDa N-terminal domain, these can selectively traffic to different rows. Using isoform-specific knockout mouse, we show expressing only small isoform...
Abstract Dynamic regulation of histone methylation by methyltransferases and demethylases plays a central role in regulating the fate embryonic stem (ES) cells. The H3K9 methyltransferase KMT1E, formerly known as ESET or Setdb1, is essential to development ablation Setdb1 gene results peri-implantation lethality prevents propagation ES However, Setdb1-null blastocysts do not display global changes DNA methylation, arguing against genome-wide defect. Here we show that conditional deletion...
Astrocytes are one of the most abundant cell types in mammalian central nervous system, and astrocyte swelling is primary event associated with brain edema. Glutamate, principal excitatory amino acid neurotransmitter released at high levels after injury including cerebral ischemia. This leads to swelling, which we previously demonstrated related metabotropic glutamate receptor (mGluR) activation. Aquaporin 4 (AQP4), predominant water channel brain, expressed endfeet plays an important role...
Non-alcoholic fatty liver disease (NAFLD) represents a complex complication of type 2 diabetes mellitus (T2DM). Oxymatrine (OMT) is an alkaloid extracted from Sophora flavescens with broad pharmacological effects. However, there currently lack research on OMT in the field NAFLD. The present study aimed to explore effects and underlying mechanisms oxymatrine treating T2DM mice model was induced by high-fat diet (HFD) combined streptozotocin (STZ) injection male C57BL/6 J mice. Animals were...
The mouse tubby phenotype is characterized by maturity-onset obesity accompanied retinal and cochlear degeneration. A positional cloning effort to find the gene responsible for this led identification of tub, a member novel family unknown function. splice defect mutation in 3' end tub gene, predicted disrupt C terminus Tub protein, has been implicated genesis phenotype. It not clear, however, whether mutant protein retains any biological activity, or perhaps some dominant function, nor it...
The absence of thyroid hormone (TH) during late gestation and early infancy can cause irreparable deafness in both humans rodents. A variety rodent models have been used an effort to identify the underlying molecular mechanism. Here, we characterize a mouse model secondary hypothyroidism, pituitary transcription factor 1 ( Pit1 dw ), which has profound, congenital that is rescued by oral TH replacement. These mutants tectorial membrane abnormalities, including prominent Hensen's stripe,...
Posttraumatic stress disorder (PTSD) is an anxiety characterized by intrusive recollections of a severe traumatic event and hyperarousal following exposure to the event. Human animal studies have shown that change amygdala activity after may contribute occurrences some symptoms or behaviors patients animals with PTSD. However, it still unknown how neuronal activation different sub-regions in changes during development In present study, we used single prolonged (SPS) procedure obtain model...
The transcription factor OTX2 is implicated in ocular, craniofacial, and pituitary development.
Summary Introduction Mutations in the transcription factor HESX 1 can cause isolated growth hormone deficiency ( IGHD ) or combined pituitary CPHD with without septo‐optic dysplasia SOD ). So far there is no clear genotype–phenotype correlation. Patients and Results We report four different recessive loss‐of‐function mutations three unrelated families midline defects . A homozygous p.R160C mutation was found by Sanger sequencing two siblings from a consanguineous family. These patients...
Chronic renal failure (CRF) is a major public health problem worldwide. In this work, we investigated the effects of purified
Abstract Introduction This study mainly investigated the role of miR‐199a‐5p in depression. Methods qRT‐PCR and western blotting were employed to detect expressions miR‐199a‐5p, CREB BDNF. Sucrose preference test, forced swimming tail suspension test performed evaluate depression‐related symptoms. MTT assays flow cytometry used examine cell reproduction apoptotic cells hippocampal neuron. Results The data demonstrated that expression levels cerebrospinal fluids serums depression patient...
Increased blood levels of low-density lipoprotein cholesterol (LDL-C) and fibrinogen are independent risk factors for cardiovascular disease. We identified associations between an Amish-enriched missense variant (p.Asn352Ser) in a functional domain beta-1,4-galactosyltransferase 1 (B4GALT1) 13.9 milligrams per deciliter lower LDL-C (P = 4.1 × 10–19) 29 plasma 1.3 10–5). B4GALT1 gene–based analysis 544,955 subjects showed association with decreased coronary artery disease (odds ratio 0.64, P...
This study aimed to investigate the effect and underlying mechanism of a purified Laminaria japonica polysaccharide (LJP61A) on preventing vascular calcification (VC). In adenine-induced chronic renal failure (CRF) mice VC model β-glycerophosphate (β-GP)-induced smooth muscle cells (VSMC) model, LJP61A was found significantly inhibit phenotypes as determined by biochemical analysis von Kossa, alizarin red, immunohistochemical staining. Meanwhile, remarkably up-regulated mRNA levels VSMC...
Functional maturation of afferent synaptic connections to inner hair cells (IHCs) involves pruning excess synapses formed during development, as well the strengthening and survival retained synapses. These events take place thyroid hormone (TH)-critical period cochlear which is in perinatal for mice third trimester humans. Here, we used hypothyroid Snell dwarf mouse (Pit1(dw)) a model study role TH type I refinement functional maturation. We observed defects delays calcium channel clustering...