A5062179607- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Parasites and Host Interactions
- Cytomegalovirus and herpesvirus research
- Parasite Biology and Host Interactions
- Parasitic Infections and Diagnostics
- Rabies epidemiology and control
- Helminth infection and control
- Trypanosoma species research and implications
- Viral Infections and Vectors
- Parasitic Diseases Research and Treatment
- Impact of Light on Environment and Health
- Drug-Induced Hepatotoxicity and Protection
- Diet, Metabolism, and Disease
- Liver Disease Diagnosis and Treatment
- Traffic and Road Safety
- Identification and Quantification in Food
- Immune cells in cancer
- Mollusks and Parasites Studies
- Liver physiology and pathology
- Marine Ecology and Invasive Species
- Pneumocystis jirovecii pneumonia detection and treatment
- Polymer Nanocomposites and Properties
- interferon and immune responses
- Human-Automation Interaction and Safety
Zhejiang University of Technology
2010-2024
Hangzhou Medical College
2021-2023
Zhejiang Academy of Medical Sciences
2009-2021
Zhejiang University
2020
Jiangsu Institute of Parasitic Diseases
2015-2019
Signify (Netherlands)
2019
Southeast University
2019
Chinese Academy of Medical Sciences & Peking Union Medical College
2011-2015
Hangzhou Academy of Agricultural Sciences
2015
Abstract Background Toxoplasmosis is a widespread zoonotic parasitic disease that occurs in both animals and humans. Traditional molecular assays are often difficult to perform, especially for the early diagnosis of Toxoplasma gondii infections. Here, we established novel loop-mediated isothermal amplification targeting 529 bp repeat element ( -LAMP) detect T. DNA blood samples experimental mice infected with tachyzoites RH strain. Findings The assay was performed Bst polymerase at 65°C 1 h....
Non-alcoholic fatty liver disease (NAFLD) represents a complex complication of type 2 diabetes mellitus (T2DM). Oxymatrine (OMT) is an alkaloid extracted from Sophora flavescens with broad pharmacological effects. However, there currently lack research on OMT in the field NAFLD. The present study aimed to explore effects and underlying mechanisms oxymatrine treating T2DM mice model was induced by high-fat diet (HFD) combined streptozotocin (STZ) injection male C57BL/6 J mice. Animals were...
Toxoplasma gondii causes zoonotic infections in a wide range of intermediate hosts and remains threatening disease worldwide because the lack effective drugs vaccines. Dense granule protein 24 (GRA24) is novel essential virulence factor that transferred into nucleus host cells from parasitophorous vacuole to regulate gene expression. In present study, bioinformatic analysis showed GRA24 had high score for B-cell T-cell epitopes compared with surface antigen 1 (SAG1), which has been studied...
Abstract Background Angiostrongylus cantonensis is a zoonotic parasite that causes eosinophilic meningitis in humans. The most common source of infection with A. the consumption raw or undercooked mollusks (e.g., snails and slugs) harbouring infectious third-stage larvae (L 3 ). However, difficult to identify snails. purpose this study was develop quick, simple molecular method survey for intermediate host Findings We used loop-mediated isothermal amplification (LAMP) assay, which performed...
Abstract Background Schistosomiasis japonica (schistosomiasis) is a zoonosis that can seriously affect human health. At present, the immunodiagnostic assays for schistosomiasis detection are time-consuming and require well-trained personnel special instruments, which limit their use in field. Thus, there pressing need simple rapid immunoassay to screen patients on large scale. In this study, we developed novel dipstick with latex immunochromatographic assay (DLIA) detect anti- Schisaosoma...
Toxoplasma gondii is an obligate intracellular protozoan parasite that causes toxoplasmosis and threatens warm-blooded animal human health worldwide. Simple applicable diagnostic methods are urgently needed to guide development of effective approaches for prevention toxoplasmosis. Most molecular tools T. infection require high technical skills, sophisticated equipment, a controlled lab environment. In this study, we developed loop-mediated isothermal amplification-lateral-flow-dipstick...
Abstract To investigate the dispersion and nanofillers' interaction of rod‐like silicates (attapulgite, ATT) in polymethylmethacrylate (PMMA) matrix, a novel situ modification ATT by toluene‐2,4‐di‐isocyanate (TDI) using mechanical mixing was exploited, which resulted homogeneous texture nanorods. As consequence, organo‐modified ATT/PMMA nanocomposites were prepared, provided prominent improvements strength, toughness, thermal stability. High grafting efficiency TDI on surface confirmed FTIR...
Toxoplasma gondii (T. gondii) is distributed worldwide and infects most species of warm-blooded animals, including humans. Toxoplasmosis has serious consequences, especially in people with an impaired or immature immune system. Thus, effective vaccine urgently required. Secretory microneme proteins are essential for the adhesion invasion T. gondii. The gene encoding protein, secreted protein altered thrombospondin repeat (TgSPATR), we constructed a recombinant eukaryotic plasmid,...
To evaluate a modified rapid ELISA method for detecting CAg during Toxoplasma gondii infection, we analyzed the specificity and sensitivity of by using experimental infection in rabbits also tested this human samples including 5428 serum, 548 cerebrospinal fluid two breast milk samples. We prepared PcAb, used it one-step sandwich testing which an incubation time regular procedure was omitted. This detected at concentration 31.2 ng/mL, no cross-reaction found with antigens protozoa...
Toxoplasma gondii causes serious public health problems, but there is no effective treatment strategy against it currently. DNA vaccines have shown promising findings in this regard. MYR1 a new virulence factor identified T. that may potential as vaccine candidate. We constructed recombinant eukaryotic plasmid, pVAX1-MYR1, vaccine, injected intramuscularly into BALB/c mice, and evaluated its immunoprotective effects. pVAX1-MYR1 immunization induced sequential Th1 Th2 T-cell response,...
Toxoplasma gondii is an obligate intracellular apicomplexan parasite and responsible for zoonotic toxoplasmosis. It essential to develop effective anti-T. vaccine the control of toxoplasmosis, this study explore immunoprotective effects a live attenuated in mice cats.First, ompdc uprt genes T. were deleted through CRISPR-Cas9 system. Then, proliferation virulence mutant strain evaluated. Subsequently, immune responses induced by cats detected, including antibody titers, cytokine levels,...
Background Artesunate (ART) has been reported to have an antifibrotic effect in various organs. The underlying mechanism not systematically elucidated. We aimed clarify the of ART on liver fibrosis induced by Schistosoma japonicum ( S . ) experimentally infected rodent model and potential mechanisms. Methods hepatic stellate cells (HSCs) was assessed using CCK-8 Annexin V-FITC/PI staining assays. experimental established Mongolian gerbil with cercariae then treated 20 mg/kg or 40 ART....
Toxoplasmosis, caused by Toxoplasma gondii, is associated with several clinical syndromes, including encephalitis, chorioretinitis, and congenital infection. gondii a ubiquitous apicomplexan parasite found in both humans animals. Mongolian gerbils, which are more susceptible to high- low-virulence strains compared mice, considered useful models for assessing diagnosis treatment methods toxoplasmosis, as well infection host defense this organism. Here we established quantitative real-time...
We applied isobaric tagging reagents for relative and absolute quantification analyses to investigate the effect of artesunate on proteome of<italic>Schistosoma japonicum</italic>in susceptible mice.
miRNAs play a significant role in pharmacogenomics and are likely to be important the molecular mechanism of atesunate (ART) effects on Schistosoma japonicum.We sequenced RNAs using an Illumina (Solexa) DNA sequencer compared relative expression levels 10-day-old schistosomula from ART parallel control group.We characterized 95 known S. japonicum individuals, including 38 novel miRNA families. Among detectable 134 differentially expressed (>2.0-fold change, p < 0.01) after treatment...
Toxoplasma gondii is an obligate intracellular protozoan parasite. T. primarily infection in pregnant women may result fetal abortion, and immunosuppressed population toxoplasmosis. Carbamoyl phosphate synthetase II (CPSII) a key enzyme the de novo pyrimidine-biosynthesis pathway, has crucial role parasite replication. We generated mutant with complete deletion of CPSII via clustered regularly interspaced short palindromic repeats (CRISPR)/cas9 type-1 RH strain . tested proliferation this...
Early diagnosis of trichinellosis is still difficult because the lack specific symptoms and limited window for serological detection. Here we established an assay based on tracing phosphate ions generated during loop-mediated isothermal amplification (LAMP) to detect Trichinella spiralis DNA in rat feces its early stage infection. By targeting a 1.6-kb repetitive element Tri. spiralis, was able all infected rats as 1 day postinfection (dpi). The positive detection lasted 7 dpi with 250...
Existing models to predict night-time target visibility on a road are mainly based psychophysical experiments conducted in laboratory environments, which may not be representative during actual driving conditions.Target visibility, under mesopic conditions, with as workload is investigated this paper by using simulator.Results show that leads reduced detection rate and an increased reaction time, means impaired conditions.The corresponding levels (VL) calculated for different workloads.The...
LED road lighting has been installed in many cities and it's dominating for almost all new roads. Its advantages have well recognized, such as improved energy efficiency, better beam control, slim design, smart etc. However, visual discomfort one of the often-heard complaints about lights since very long ago. Visual can be attributed to three factors general: disability/discomfort glare, surface uniformity, vertical illuminance distribution. For glare there abundant studies standards...