A5055009607- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Parasites and Host Interactions
- Rabies epidemiology and control
- Engineering Education and Curriculum Development
- Parasite Biology and Host Interactions
- Education and Work Dynamics
- interferon and immune responses
- Bacteriophages and microbial interactions
- Higher Education and Teaching Methods
- Mosquito-borne diseases and control
- Parasitic Diseases Research and Treatment
- Cancer, Hypoxia, and Metabolism
- Viral Infections and Vectors
- EFL/ESL Teaching and Learning
- HIV Research and Treatment
- University-Industry-Government Innovation Models
- Ubiquitin and proteasome pathways
- Protein Tyrosine Phosphatases
- Zoonotic diseases and public health
- Epigenetics and DNA Methylation
- Pain Management and Placebo Effect
- Globalization, Economics, and Policies
- Insect symbiosis and bacterial influences
Nanjing University
2023-2025
Hangzhou Medical College
2021-2024
Vector (United States)
2024
Fujian Medical University
2018-2024
Union Hospital
2024
National Institute for Parasitic Diseases
2019-2024
Hospital for Tropical Diseases
2024
Wuxi Taihu Hospital
2021-2022
China University of Mining and Technology
2021-2022
Zhejiang Academy of Medical Sciences
2015-2021
Abstract Background Toxoplasmosis is a widespread zoonotic parasitic disease that occurs in both animals and humans. Traditional molecular assays are often difficult to perform, especially for the early diagnosis of Toxoplasma gondii infections. Here, we established novel loop-mediated isothermal amplification targeting 529 bp repeat element ( -LAMP) detect T. DNA blood samples experimental mice infected with tachyzoites RH strain. Findings The assay was performed Bst polymerase at 65°C 1 h....
Viral adhesion to host cells is a critical step in infection for many viruses, including monkeypox virus (MPXV). In MPXV, the H3 protein mediates viral through its interaction with heparan sulfate (HS), yet structural details of this have remained elusive. Using AI-based prediction tools and molecular dynamics (MD) simulations, we identified novel, positively charged α-helical domain that essential HS binding. This conserved domain, found across orthopoxviruses , was experimentally validated...
Toxoplasma gondii causes zoonotic infections in a wide range of intermediate hosts and remains threatening disease worldwide because the lack effective drugs vaccines. Dense granule protein 24 (GRA24) is novel essential virulence factor that transferred into nucleus host cells from parasitophorous vacuole to regulate gene expression. In present study, bioinformatic analysis showed GRA24 had high score for B-cell T-cell epitopes compared with surface antigen 1 (SAG1), which has been studied...
Abstract Background Timely diagnosis of Toxoplasma gondii infection is necessary to prevent and control toxoplasmosis transmission. The gold immunochromatographic assay (GICA) a means rapidly detecting pathogen in samples. GICA-based diagnostic methods have been developed accurately detect pathogens with high sensitivity specificity, their application T. expected yield good results. Methods Colloidal test strips were produced using C-terminal truncated apical membrane antigen 1 (AMA1C)....
, which causes toxoplasmosis, is prevalent in warm-blooded animals, such as cats, dogs, and humans.
Toxoplasma gondii is an obligate intracellular protozoan parasite that causes toxoplasmosis and threatens warm-blooded animal human health worldwide. Simple applicable diagnostic methods are urgently needed to guide development of effective approaches for prevention toxoplasmosis. Most molecular tools T. infection require high technical skills, sophisticated equipment, a controlled lab environment. In this study, we developed loop-mediated isothermal amplification-lateral-flow-dipstick...
Toxoplasma gondii (T. gondii) is distributed worldwide and infects most species of warm-blooded animals, including humans. Toxoplasmosis has serious consequences, especially in people with an impaired or immature immune system. Thus, effective vaccine urgently required. Secretory microneme proteins are essential for the adhesion invasion T. gondii. The gene encoding protein, secreted protein altered thrombospondin repeat (TgSPATR), we constructed a recombinant eukaryotic plasmid,...
BACKGROUND:Many findings have shown that pyruvate kinase type M2 (PKM2) plays crucial roles in regulating the occurrence and development of various human cancers; however, its ovarian cancer oncogenesis remain to be determined. MATERIAL AND METHODS:The expression intensity PKM2 tissues was examined by immunohistochemistry (IHC), then correlated patient clinicopathologic characteristics. The cell proliferation, growth, survival were CCK-8, colony forming, flow cytometry assays. potentially...
Toxoplasma gondii causes serious public health problems, but there is no effective treatment strategy against it currently. DNA vaccines have shown promising findings in this regard. MYR1 a new virulence factor identified T. that may potential as vaccine candidate. We constructed recombinant eukaryotic plasmid, pVAX1-MYR1, vaccine, injected intramuscularly into BALB/c mice, and evaluated its immunoprotective effects. pVAX1-MYR1 immunization induced sequential Th1 Th2 T-cell response,...
Toxoplasma gondii is an obligate intracellular apicomplexan parasite and responsible for zoonotic toxoplasmosis. It essential to develop effective anti-T. vaccine the control of toxoplasmosis, this study explore immunoprotective effects a live attenuated in mice cats.First, ompdc uprt genes T. were deleted through CRISPR-Cas9 system. Then, proliferation virulence mutant strain evaluated. Subsequently, immune responses induced by cats detected, including antibody titers, cytokine levels,...
Abstract Background Following China’s official designation as malaria-free country by WHO, the imported malaria has emerged a significant determinant impacting reestablishment within China. The objective of this study is to explore application prospects machine learning algorithms in risk assessment Methods data cases China from 2011 2019 was provided CDC; historical epidemic endemic obtained World Malaria Report, and other used are open access data. All processing model construction based...
Abstract Background Diseases caused by Toxoplasma gondii ( T. ) have introduced serious threats to public health . There is an urgent need develop a rapid detection method for infection in cats, which are definitive hosts. Recombinant apical membrane antigen 1 (rAMA1) was produced prokaryotic expression system and used as the antigen. The aim of this study evaluate optimize reliable indirect enzyme-linked immunosorbent assay (iELISA) based on rAMA1 antibodies against cats. Results...
, an obligate intracellular eukaryotic parasite, can infect about one-third of the world's population. One vaccine, Toxovax, has been developed and licensed commercially; however, it is only used in sheep industry to reduce losses caused by congenital toxoplasmosis. Various other vaccine approaches have explored, including excretory secretion antigen vaccines, subunit epitope DNA vaccines. However, current research not yet a safe effective for
We applied isobaric tagging reagents for relative and absolute quantification analyses to investigate the effect of artesunate on proteome of<italic>Schistosoma japonicum</italic>in susceptible mice.
miRNAs play a significant role in pharmacogenomics and are likely to be important the molecular mechanism of atesunate (ART) effects on Schistosoma japonicum.We sequenced RNAs using an Illumina (Solexa) DNA sequencer compared relative expression levels 10-day-old schistosomula from ART parallel control group.We characterized 95 known S. japonicum individuals, including 38 novel miRNA families. Among detectable 134 differentially expressed (>2.0-fold change, p < 0.01) after treatment...