A5082040535- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Viral Infectious Diseases and Gene Expression in Insects
- Renal and related cancers
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- 3D Printing in Biomedical Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Infant Nutrition and Health
- Retinal Development and Disorders
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- CAR-T cell therapy research
- Genetic Associations and Epidemiology
- Metabolism and Genetic Disorders
- Genetic Mapping and Diversity in Plants and Animals
- Genetic Neurodegenerative Diseases
- Cell Adhesion Molecules Research
- Digestive system and related health
- Amyotrophic Lateral Sclerosis Research
- Blood properties and coagulation
- Osteoarthritis Treatment and Mechanisms
Regeneron (United States)
2013-2024
UConn Health
2010-2011
Hartford Financial Services (United States)
2010
Evergen Biotechnologies (United States)
2010
University of Connecticut
2010
China Agricultural University
2004-2008
Many studies are uncovering functional roles for long noncoding RNAs (lncRNAs), yet few have been tested in vivo relevance through genetic ablation animal models. To investigate the of lncRNAs various physiological conditions, we developed a collection 18 lncRNA knockout strains which locus is maintained transcriptionally active. Initial characterization revealed peri- and postnatal lethal phenotypes three mutant (Fendrr, Peril, Mdgt), latter two exhibiting incomplete penetrance growth...
The expansion of a hexanucleotide (GGGGCC) repeat in C9ORF72 is the most common cause amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Both function mechanism by which drives neuropathology are unknown. To examine whether haploinsufficiency induces neurological disease, we created C9orf72-deficient mouse line. Null mice developed robust immune phenotype characterized myeloid expansion, T cell activation, increased plasma cells. Mice also presented with elevated...
Large-scale production of biopharmaceuticals by current bioreactor techniques is limited low transgenic efficiency and expression foreign proteins. In general, a bacterial artificial chromosome (BAC) harboring most regulatory elements capable overcoming the limitations, but transferring BAC into donor cells difficult. We describe here use cattle mammary to produce functional recombinant human lactoferrin (rhLF) novel procedure cloning, which employs microinjection generate somatic as cells....
In a survey of 20 knockout mouse lines designed to examine the biological functions large intergenic non-coding RNAs (lincRNAs), we have found variety phenotypes, ranging from perinatal lethality defects associated with premature aging and morphological functional abnormalities in lungs, skeleton, muscle. Each mutant allele carried lacZ reporter whose expression profile highlighted wide spectrum spatiotemporal tissue-specific transcription patterns embryos adults that informed our phenotypic...
Expansion of a G4C2 repeat in the C9orf72 gene is associated with familial Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD). To investigate underlying mechanisms instability, which occurs both somatically intergenerationally, we created novel mouse model ALS/FTD that harbors 96 copies repeats at humanized locus. In embryonic stem cells, observed two modes expansion. First, noted minor increases length per expansion event, was dependent on mismatch repair pathway protein...
Improvement of the nutritional value cow milk with transgenic expression recombinant human α-lactalbumin (α-LA) has been previously attempted. However, detailed characterization protein and analysis components are not explored yet. Here, we first report production healthy cows by somatic cell nuclear transfer, in which up to 1.55 g/L α-LA was achieved. The purified from displayed physicochemical properties similar its natural counterpart respect molecular weight, structure, regulatory...
Treatment of common and debilitating degenerative cartilage diseases particularly osteoarthritis is a clinical challenge because the limited capacity tissue for self-repair. Because their unlimited self-renewal ability to differentiate into multiple lineages, human embryonic stem cells (hESCs) are potentially powerful tool repair defects. The primary objective present study was develop culture systems conditions that enable hESCs directly uniformly chondrogenic lineage without prior embryoid...
Abstract The present study examined effects of genetic manipulation and serum starvation on in vitro developmental potential bovine somatic cell nuclear transfer (SCNT) embryos vitrification vivo competence transgenic SCNT blastocysts. Fetal oviduct epithelial cells (FOECs) were isolated from the a Day 147 fetus transfected with plasmid (pCE‐EGFP‐IRES‐NEO) containing enhanced green fluorescent protein ( EGFP ) neomycin‐resistant Neor genes. There no significant differences P > 0.05)...
The goals of the current study were to (1) improve culture conditions and (2) chemical passaging bovine embryonic stem cell-like (bESC-like) cells. Specifically, effects human leukemia inhibitory factor (hLIF), two types feeders, mouse fibroblast (MEF) (BEF), as well three different enzymatic treatments including Trypsin-EDTA, TrypLE, Liberase Blendzymes 3 investigated. addition hLIF at 1000 U/mL medium (41.2 36.9%), use either MEF or BEF feeders (40.3 38.1%) had no significant effect on...
CRISPR-based transcriptional activation is a powerful tool for functional gene interrogation; however, delivery difficulties have limited its applications in vivo. Here, we created mouse model expressing all components of the CRISPR-Cas9 guide RNA-directed Synergistic Activation Mediator (SAM) from single transcript that capable activating target genes tissue-specific manner. We optimized Lipid Nanoparticles and Adeno-Associated Virus RNA approaches to achieve expression modulation one or...
Increased blood levels of low-density lipoprotein cholesterol (LDL-C) and fibrinogen are independent risk factors for cardiovascular disease. We identified associations between an Amish-enriched missense variant (p.Asn352Ser) in a functional domain beta-1,4-galactosyltransferase 1 (B4GALT1) 13.9 milligrams per deciliter lower LDL-C (P = 4.1 × 10–19) 29 plasma 1.3 10–5). B4GALT1 gene–based analysis 544,955 subjects showed association with decreased coronary artery disease (odds ratio 0.64, P...
The aim of this study was to investigate the efficiency in vitro embryo production cattle utilizing sexed sperm from two bulls and oocytes recovered by OPU. Twenty donor animals were employed eight OPU replicates: first four trials conducted on without hormone treatment, last run same animals, following FSH subcutaneous intramuscular administration. A higher rate blastocyst development recorded stimulated, as compared nonstimulated (25.2% versus 12.8%, ). Ocytes derived slaughterhouse (SH)...
The Adisintegrin and metalloprotease domain-containing (ADAM) family of proteins is involved in cell adhesion, migration, proteolysis, signaling. Many ADAMs are required for reproduction; however, the role Adam6 has remained largely unknown. In course humanizing mouse immunoglobulin heavy chain (IgH) locus, we generated Adam6-deficient mice that demonstrate severe subfertility. We decided to elucidate ADAM6 fertility explore underlying mechanisms. Despite normal sperm development motility,...
Abstract The incidence of liver cancer is growing worldwide, and it estimated that by 2025, over 1 million individuals will be affected every year. Hepatocellular carcinoma (HCC) accounts for about 90% primary cases one the leading causes death from worldwide. Establishing animal models faithfully replicate human disease critical to improve our understanding HCC pathogenesis test new therapeutic strategies. Here, we describe a novel model using genetically modified hepatocytes in humanized...