A5035307849- Angiogenesis and VEGF in Cancer
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Lipid metabolism and disorders
- Blood properties and coagulation
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Diabetes and associated disorders
- Evolution and Paleontology Studies
- Stress Responses and Cortisol
- Apelin-related biomedical research
- HIV Research and Treatment
- Genetic Associations and Epidemiology
- Parasitic Infections and Diagnostics
- Physiological and biochemical adaptations
- Toxoplasma gondii Research Studies
- Porphyrin Metabolism and Disorders
- Race, Genetics, and Society
- Erythrocyte Function and Pathophysiology
- Bat Biology and Ecology Studies
- Freezing and Crystallization Processes
- Atherosclerosis and Cardiovascular Diseases
- Food Industry and Aquatic Biology
- Helminth infection and control
Regeneron (United States)
1997-2021
University of the Witwatersrand
1991-2007
University College Cork
2007
Repligen (United States)
1993-1995
University of Minnesota
1994
Angiogenesis is thought to depend on a precise balance of positive and negative regulation. Angiopoietin-1 (Ang1) an angiogenic factor that signals through the endothelial cell–specific Tie2 receptor tyrosine kinase. Like vascular growth factor, Ang1 essential for normal development in mouse. An relative, termed angiopoietin-2 (Ang2), was identified by homology screening shown be naturally occurring antagonist Tie2. Transgenic overexpression Ang2 disrupts blood vessel formation mouse embryo....
Angiopoietin (Ang)-2, a context-dependent agonist/antagonist for the vascular-specific Tie2 receptor, is highly expressed by endothelial cells at sites of normal and pathologic angiogenesis. One prevailing model suggests that in these settings, Ang-2 acts as an autocrine blocker, inhibiting stabilizing influence activator Ang-1, thereby promoting vascular remodeling. However, effects endogenous on are actively producing it have not been studied detail. Here, we demonstrate expression rapidly...
Detection and quantitation of homodimer impurities in therapeutic bispecific antibody (bsAb) drug products is essential to support development quality control (QC) release. LC-MS-based techniques have been frequently applied for this analysis. However, sensitive detection low-abundance can still be challenging regular workflows, which largely due the lack chromatographic resolution between main bsAb species. Here, we report a novel analytical method, couples mixed-mode size exclusion...
The proliferation of human myeloid progenitor cells is negatively regulated in the presence certain members chemokine family molecules. This includes interleukin 8 (IL-8) and platelet factor 4 (PF4), which combination are able to synergize, resulting cell suppression at very low concentrations these A series PF4 IL-8 mutant proteins were analyzed an vitro colony formation assay for assess domains that required activity. Mutation either two DLQ motifs within resulted inactive protein....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDissociation of the lactose repressor protein tetramer using high hydrostatic pressureCatherine A. Royer, Gregorio Weber, Thomas J. Daly, and Kathleen Shive MatthewsCite this: Biochemistry 1986, 25, 8308–8315Publication Date (Print):December 16, 1986Publication History Published online1 May 2002Published inissue 16 December 1986https://pubs.acs.org/doi/10.1021/bi00373a027https://doi.org/10.1021/bi00373a027research-articleACS PublicationsRequest...
Traditional SDS-PAGE method and its modern equivalent CE-SDS are both widely applied to assess the purity of therapeutic monoclonal antibody (mAb) drug products. However, structural identification low molecular weight (LMW) impurities using those methods has been challenging largely based on empirical knowledges. In this paper, we present that hydrophilic interaction chromatography (HILIC) coupled with mass spectrometry analysis is a novel orthogonal characterize such LMW within purified mAb...
Cold acclimation induces very divergent responses in thyroid function reptiles and mammals reflective of their different thermoregulatory modes. Naked mole-rats, unlike other small mammals, are unable to effectively employ endothermy operatively poikilotherms. We therefore investigated changes status with chronic cold exposure. Under simulated burrow conditions, free thyroxine (T 4 ; 0.39 ± 0.09 ng/dl) stimulating hormone (TSH; 1.12 0.56 μIU/ml) levels fell within the reptilian range, one...
To evaluate the accuracy of Quantikine Human VEGF Immunoassay (R&D Systems) in presence inhibitors.Quantikine ELISA (R&D), anti-VEGF165 mAb VEGF165 and aflibercept (Regeneron), ranibizumab bevacizumab (Genentech).Binding affinity for was threefold weaker than aflibercept, but 33- 40-fold stronger or bevacizumab. Extended incubation complexed with inhibitors led to dissociation from bevacizumab, not subsequent binding by immunoassay capture antibody. The also detected VEGF:ranibizumab...
Increased blood levels of low-density lipoprotein cholesterol (LDL-C) and fibrinogen are independent risk factors for cardiovascular disease. We identified associations between an Amish-enriched missense variant (p.Asn352Ser) in a functional domain beta-1,4-galactosyltransferase 1 (B4GALT1) 13.9 milligrams per deciliter lower LDL-C (P = 4.1 × 10–19) 29 plasma 1.3 10–5). B4GALT1 gene–based analysis 544,955 subjects showed association with decreased coronary artery disease (odds ratio 0.64, P...
The skin structure of 2 Bathyergid rodents, the naked mole‐rat ( Heterocephalus glaber ) and common Cryptomys hottentotus is compared, to investigate whether thermoregulatory differences may be attributed different features. Histological ultrastructural studies dorsal these closely related species show morphological structural similarities but in degree folding, thickness integument dermal infrastructure were evident. conforms with expected morphological/histological arrangements that are...
Platelet basic protein (PBP) (94 residues) is naturally processed via N-terminal cleavage to yield connective tissue activating peptide-III (85 residues), beta-thromboglobulin (81 and neutrophil peptide-2 (70 residues). Chemical cross-linking gel filtration data indicate that each homolog can form dimers tetramers. Subunit association equilibria for dimer (KD) tetramer (KT) formation have been derived species from 1H NMR (600 MHz) spectral analysis of slowly exchanging (NMR time scale)...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCharacterization and modification of a monomeric mutant the lactose repressor proteinThomas J. Daly Kathleen Shive MatthewsCite this: Biochemistry 1986, 25, 19, 5474–5478Publication Date (Print):September 23, 1986Publication History Published online1 May 2002Published inissue 23 September 1986https://pubs.acs.org/doi/10.1021/bi00367a019https://doi.org/10.1021/bi00367a019research-articleACS PublicationsRequest reuse permissionsArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAllosteric regulation of inducer and operator binding to the lactose repressorThomas J. Daly Kathleen Shive MatthewsCite this: Biochemistry 1986, 25, 19, 5479–5484Publication Date (Print):September 23, 1986Publication History Published online1 May 2002Published inissue 23 September 1986https://pubs.acs.org/doi/10.1021/bi00367a020https://doi.org/10.1021/bi00367a020research-articleACS PublicationsRequest reuse permissionsArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFormation of mixed disulfide adducts at cysteine-281 the lactose repressor protein affects operator and inducer binding parametersThomas J. Daly, John S. Olson, Kathleen Shive MatthewsCite this: Biochemistry 1986, 25, 19, 5468–5474Publication Date (Print):September 23, 1986Publication History Published online1 May 2002Published inissue 23 September 1986https://doi.org/10.1021/bi00367a018RIGHTS & PERMISSIONSArticle Views94Altmetric-Citations28LEARN...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPerturbation of the carboxy terminus HIV-1 Rev affects multimerization on responsive elementThomas J. Daly, Paul Rennert, Lynch, Jennifer K. Barry, Moira Dundas, James R. Rusche, Reed C. Doten, Manfred Auer, and G. King FarringtonCite this: Biochemistry 1993, 32, 34, 8945–8954Publication Date (Print):August 31, 1993Publication History Published online1 May 2002Published inissue 31 August...
Plant sterols are specific phytochemicals that resemble cholesterol in structure but found exclusively plants [1]. The most common phytosterols the human diet β-sitosterol, campesterol and stigmasterol. Although plant have been described as immunomodulatory compounds [2] there a limited number of studies reporting effects these on immune modulation. In present study we investigated β-sitosterol cytokine release mitogen-treated Jurkat cells. Human T cells (2×105/ml) were supplemented with...
The thermogenic potential of the interscapular brown fat pad in naked mole‐rat Heterocephalus glaber , that exhibits poikilothermic thermal responses to changing temperatures is reported. Histological and ultrastructural study showed it consists layers skeletal muscle interposed between adipose tissue with both unilocular multilocular adipocytes. Large numbers mitochondria were present around lipid droplets these cells. Glyoxylic acid condensation, used demonstrate catecholaminergic nerves,...
Changes in vaginal epithelium are known to occur during the normal oestrous or menstrual cycle and ovariectomised animals response various hormones. However, changes due exogenous gonadotrophins superimposed on hormonal milieu as occurs vitro fertilisation programmes have not previously been demonstrated. Female rats were hyperstimulated with follicle stimulating hormone human chorionic gonadotrophin prior mating. Control mated injected. Vaginal tissue was collected at 4.5, 5.5 6.5 d after...
Abstract Increased LDL-cholesterol (LDL-C) and fibrinogen are independent risk factors for cardiovascular disease (CVD). We identified novel associations between an Amish-enriched missense variant (p.Asn352Ser) in a functional domain of beta-1,4-galactosyltransferase 1 ( B4GALT1 ) 13.5 mg/dl lower LDL-C (p=1.6E-15), 26 plasma (p= 9.8E-05). N-linked glycan profiling found p.Asn352Ser to be associated (p-values from 1.4E-06 1.0E-17) with decreased glycosylation glycoproteins including:...
This paper examines the research on race determination conducted by Russian biochemist E.O. Manoiloff in 1920s. claimed to have discovered a method which detected racial identity of an individual simple chemical reaction performed subject's blood sample. The was published one leading anthropological journals and it not questioned for some time. It is obvious today that Manoiloff's claims were nothing short ridiculous. present study, based experimental history sciences, tries elucidate...