A5036686131- Plant nutrient uptake and metabolism
- GABA and Rice Research
- Rice Cultivation and Yield Improvement
- RNA and protein synthesis mechanisms
- Plant responses to water stress
- Genetic Mapping and Diversity in Plants and Animals
- Bacterial Genetics and Biotechnology
- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Microbial Metabolic Engineering and Bioproduction
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- RNA regulation and disease
- RNA modifications and cancer
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Nitrogen and Sulfur Effects on Brassica
South China Agricultural University
2024
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2024
Hunan University
2017-2021
Hunan Agricultural University
2019-2021
China National Hybrid Rice R&D Central Hunan Hybrid Rice Reserch Center
2017-2020
Hunan Academy of Agricultural Sciences
2020
State Key Laboratory of Hybrid Rice
2017-2020
Hunan Rice Research Institute
2020
Education Department of Hunan Province
2019
Abstract Obtaining genetic variation information from indica rice hybrid parents and identification of loci associated with heterosis are important for breeding. Here, we resequence 1,143 accessions mostly selected the superior cultivars China, identify variations, perform kinship analysis. We find different crossing patterns between 3- 2-line lines. By calculating frequencies parental differences (FPVDs), a more direct approach studying heterosis, that linked to which include 98 in 3-line...
Panicle traits are among the most important agronomic characters which directly relate to yield in rice. Grain number (GN), panicle length (PL), primary branch (PBN), and secondary (SBN) major components of rice structure, all controlled by quantitative trait loci (QTLs). In our research, four advanced backcross overlapping populations (BIL152, BIL196a, BIL196b, BIL196b-156) carrying introgressed segments from chromosome 6 were derived an indica/japonica cross that used super-hybrid restorer...
Sulfotransferases (SOTs; EC 2.8.2.-), which are widespread from prokaryotes to eukaryotes, constitute a multi-protein family that plays crucial roles in plant growth, development and stress adaptation. However, this has not been systemically investigated Brassica rapa. Here, genome-wide systemic analysis of SOT genes B. rapa subsp. pekinensis, globally cultivated vegetable, were conducted. We identified 56 the whole genome using Arabidopsis sequences as queries classified them into nine...
Abstract Ligation-mediated PCR (LM-PCR) is a classical method for isolating flanking sequences; however, it has common limitation of reduced success rate owing to the circularization or multimerization target restriction fragments including known sequence. To address this limitation, we developed novel LM-PCR method, termed Cyclic Digestion and Ligation-Mediated (CDL-PCR). The novelty approach involves design new adapters that cannot be digested after being ligated with fragment, cyclic...
Remobilization of pre-anthesis NSCs (non-structural carbohydrates) is significant for effective grain filling in rice ( Oryza sativa L.). However, abundant starch particles as an important component are still present the leaf sheath and stem at late stage filling. There no studies on how bioengineering techniques can be used to improve efficiency NSC remobilization. In this study, RAmy1A was expressed under senescence-specific promoter SAG12 , which designed degrade during mRNA successfully...
The standard binary enzymatic assembly, which operates by inserting one DNA fragment into a plasmid, has higher assembly success rate than the polynary inserts two or more fragments plasmid. However, it often leaves nucleotide scar at junction site. When large molecule is assembled stepwise backbone plasmid in random piecewise manner, scars will damage structure of original sequence final plasmids. Here, we propose an vitro Seamless Stack Enzymatic Assembly (SSEA) method, novel method...