A5035499607- Genetic Mapping and Diversity in Plants and Animals
- GABA and Rice Research
- Rice Cultivation and Yield Improvement
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Plant Reproductive Biology
- Genetic diversity and population structure
- Trace Elements in Health
- Genetics and Plant Breeding
- Electrochemical Analysis and Applications
- Drug Transport and Resistance Mechanisms
- Parasite Biology and Host Interactions
- Animal Genetics and Reproduction
- Plant tissue culture and regeneration
- Microbial Metabolic Engineering and Bioproduction
- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Parasitic infections in humans and animals
- Bacterial Genetics and Biotechnology
- Genetics, Aging, and Longevity in Model Organisms
- Cholesterol and Lipid Metabolism
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Advanced biosensing and bioanalysis techniques
China National Hybrid Rice R&D Central Hunan Hybrid Rice Reserch Center
2017-2024
Hunan Academy of Agricultural Sciences
2017-2024
State Key Laboratory of Hybrid Rice
2015-2024
Hainan University
2024
Hunan Agricultural University
2011-2023
Hunan Rice Research Institute
2020-2023
Rice University
2015
Abstract Obtaining genetic variation information from indica rice hybrid parents and identification of loci associated with heterosis are important for breeding. Here, we resequence 1,143 accessions mostly selected the superior cultivars China, identify variations, perform kinship analysis. We find different crossing patterns between 3- 2-line lines. By calculating frequencies parental differences (FPVDs), a more direct approach studying heterosis, that linked to which include 98 in 3-line...
Climate change has caused high salinity in many fields, particularly the mud flats coastal regions. The resulting become one of most significant abiotic stresses affecting world's rice crop productivity. Developing elite cultivars with novel salinity-tolerance traits is regarded as cost-effective and environmentally friendly approach for utilizing saline-alkali land. To develop a highly efficient green strategy create germplasms salt-tolerant breeding, this study aimed to improve tolerance...
Abstract Y900 is one of the top hybrid rice (Oryza sativa) varieties, with its yield exceeding 15 t·hm−2. To dissect mechanism heterosis, we sequenced male parent line R900 and female Y58S using long-read Hi-C technology. High-quality reference genomes 396.41 Mb 398.24 were obtained for Y58S, respectively. Genome-wide variations between parents systematically identified, including 1,367,758 single-nucleotide polymorphisms, 299,149 insertions/deletions, 4,757 structural variations. The level...
"Easy-to-shatter" trait is a major cause of rice crop yield losses, emphasizing the economic value developing elite cultivars with reduced seed shattering capable achieving higher yields. In present study, we describe development new indica lines that exhibit lower rates following targeted CRISPR/Cas9-mediated editing qSH1 gene. We were able to identify mutant T0 transgenic plants, transgene-free homozygous mutants being obtained via segregation in T1 generation. then utilized two T2 order...
The ATP-binding cassette (ABC) proteins are a diverse family of transmembrane transporter widely identified in various organisms. ABCG transporters belong to the G subfamily ABC family. Rarely research on involved salt tolerance rice was found. In this study, evolutionary relationships, conserved motifs, intra- and inter-species homologous genes, cis-acting elements members were analyzed, expression changes these genes under stress at 0 h, 3 24 h detected. Based results, candidate gene
Abstract Sequence variability in three mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 3 ( cox 3), NADH dehydrogenase subunits 1 and 4 nad 4) Spirometra erinaceieuropaei spargana from different geographical regions China was examined. A portion of each the (p 1) genes were amplified separately individual S. by polymerase chain reaction (PCR). Representative amplicons subjected to sequencing order estimate sequence variability. The sequences p 3, 541, 607 847 bp length,...
Panicle traits are among the most important agronomic characters which directly relate to yield in rice. Grain number (GN), panicle length (PL), primary branch (PBN), and secondary (SBN) major components of rice structure, all controlled by quantitative trait loci (QTLs). In our research, four advanced backcross overlapping populations (BIL152, BIL196a, BIL196b, BIL196b-156) carrying introgressed segments from chromosome 6 were derived an indica/japonica cross that used super-hybrid restorer...
Abstract Ligation-mediated PCR (LM-PCR) is a classical method for isolating flanking sequences; however, it has common limitation of reduced success rate owing to the circularization or multimerization target restriction fragments including known sequence. To address this limitation, we developed novel LM-PCR method, termed Cyclic Digestion and Ligation-Mediated (CDL-PCR). The novelty approach involves design new adapters that cannot be digested after being ligated with fragment, cyclic...
The gene tms5, which controls thermo-sensitive genic male sterility (TGMS), has been widely used in two-line hybrid rice breeding China. tms5 lines have two sources, namely AnnongS-1 (AnS) and Zhu1S (ZhS) and, interestingly, are commonly subject to an alteration at cds.71. However, whether cds.71 acts as a mutation hotspot is unknown. Herein, another mutant named T98S (induced from T98B by irradiation) was explore this. First, the of tms(t) responsible for fine-mapped on chromosome 2 based...
The standard binary enzymatic assembly, which operates by inserting one DNA fragment into a plasmid, has higher assembly success rate than the polynary inserts two or more fragments plasmid. However, it often leaves nucleotide scar at junction site. When large molecule is assembled stepwise backbone plasmid in random piecewise manner, scars will damage structure of original sequence final plasmids. Here, we propose an vitro Seamless Stack Enzymatic Assembly (SSEA) method, novel method...
A maintainer line of 3-line hybrid rice commonly presents a certain genetic distance to 2-line restorer line, but in many cases, lines present defects upon recovery the object cytoplasmic male sterile (CMS) which impedes utilization their heterosis. Here, we report strategy and an example converting into photoperiod/temperature-sensitive genic (P/TGMS) with almost identical background, thus maximizing Firstly, through treatment T98B 60CO-γ irradiation, identified TGMS T98S, is at higher...
Conventional production of hybrid rice seeds is based on sowing and transplanting parental lines separately ('separate seeding transplanting', SST). To decrease the labour inputs field management, a labour-saving model was implemented under strategy directly two parents as mixture ('mixed direct seeding', MDS) to produce seeds. The utilized 'yellow-hull/red-hull' pair, ensuring that female parent could be easily separated from male after mixed harvesting. assess effects MDS seed production,...
Abstract Y900 is one of the top hybrid rice varieties with a yield exceeding 15 t/hm 2 . To dissect mechanism heterosis, male parent line R900 and female Y58S were sequenced using long-read Hi-C technology. High-quality reference genomes sizes 396.41 Mb 398.24 obtained for Y58S, respectively. Genome-wide variations between parents systematically identified, including 1,367,758 SNPs 299,149 Indels. No megabase level structural exist. >75% genes exhibited variation two parents. Compared...