A5024655029- Microfluidic and Bio-sensing Technologies
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Field-Flow Fractionation Techniques
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Orbital Angular Momentum in Optics
- Advanced biosensing and bioanalysis techniques
- Animal Virus Infections Studies
- Magnetic and Electromagnetic Effects
- Plasmonic and Surface Plasmon Research
- Heat shock proteins research
- Plant Genetic and Mutation Studies
- Spaceflight effects on biology
- Formal Methods in Verification
- Viral gastroenteritis research and epidemiology
- Micro and Nano Robotics
- Gold and Silver Nanoparticles Synthesis and Applications
- Biosensors and Analytical Detection
- Biochemical effects in animals
- Logic, Reasoning, and Knowledge
- 3D Printing in Biomedical Research
- Single-cell and spatial transcriptomics
- Software Reliability and Analysis Research
- Reproductive Health and Technologies
Max Planck University of Twente Center for Complex Fluid Dynamics
2021
University of Twente
2006-2020
Sensors (United States)
2018-2019
Pontificia Universidad Católica de Valparaíso
2009
We designed and characterized a microfluidic platform, allowing spatial confinement analysis of individually entrapped sperm cells.
Current male fertility diagnosis tests focus on assessing the quality of semen samples by studying concentration, total volume, and motility spermatozoa. However, other characteristics such as chemotactic ability a spermatozoon might influence chance fertilization. Here we describe simple, easy to fabricate handle, flow-free microfluidic chip test response spermatozoa made out hybrid hydrogel (8% gelatin/1% agarose). A chemotaxis experiment with 1 μM progesterone was performed that...
This study introduces The Potential Sensor, a proof-of-principle system designed for nocturnal erection detection. article novel approach to non-invasive ED diagnostics, which aims optimize patient comfort and consequently validity through avoidance of applying pressure-based measurements. A literature review on physiology applicable sensor technologies showed evaluated concepts measuring penile erection, including temperature, circumference, movement, blood oxygenation, arterial pulse as...
Abstract Men suffering from azoospermia can father a child, by extracting spermatozoa testicular biopsy sample. The main complication in this procedure is the presence of an abundance erythrocytes. Currently, isolation few sample manually performed due to ineffectiveness filtering methods, making it time consuming and labor intensive. are smaller both width height than any other cell type found sample, with very small difference compared erythrocyte for smallest, not feature base extraction...
The excellent specificity and selectivity of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/associated nuclease (Cas) is determined by CRISPR RNA's (crRNA's) interchangeable spacer sequence, as well the position number mismatches between target sequence crRNA sequence. Some diseases are characterized epigenetic alterations rather than nucleotide changes, therefore unsuitable for CRISPR-assisted sensing methods. Here we demonstrate an in vitro diagnostic tool to...
Pinched-flow fractionation removes viruses from (porcine) semen, which can increase the biosecurity of artificial insemination.
This paper presents an algorithm for cost-bounded probabilistic reachability in timed automata extended with prices (on edges and locations) discrete branching. The determines whether the probability to reach a (set of) goal location(s) within given price bound (and time bound) can exceed threshold p e¸ [0, 1]. We prove that is partially correct show example which termination cannot be guaranteed.
The use of microfluidics in artificial reproductive technologies for manipulation or assessment spermatozoa is unique the sense that it not always an end point measurement and sample may be used afterward. During microfluidic processing, are exposed to shear stress, which harm viability functioning spermatozoa. stresses during general processing steps were calculated compared estimated ejaculation. boar bull after was studied typical handling method (centrifugation) a control (the tube at...
The need for measurements of multiple biomarkers simultaneously at subnanomolar concentrations asks the development new sensors with high sensitivity, specificity, precision, and accuracy. Currently, multiplexed sensing in single molecule increases complexity system terms reagents sample read-out. In this letter, we propose a novel approach to multiplex hairpin-based single-DNA sensors, which overcomes limitations present approaches multiplexing. By target-dependent ssDNA hairpin design, can...
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/associated nuclease (Cas) systems have repeatedly shown to excellent performance in nucleotide sensing applications 1–5 . High specificity and selectivity of Cas effector proteins is determined by the CRISPR RNA’s (crRNA’s) interchangeable spacer sequence, as well position number mismatches between target sequence crRNA 1 Some diseases are characterized epigenetic alterations rather than changes, therefore unsuitable for...