Lacto-N-neotetraose (LNnT) and lacto-N-tetraose (LNT) are important oligosaccharides found in breast milk commonly used as nutritional supplements infant formula. We metabolic engineering techniques to optimize the modified Escherichia coli BL21 star (DE3) strain for efficient synthesis of LNnT LNT using β-1,4-galactosyltransferase (HpgalT) from Helicobacter pylori β-1,3-galactosyltransferase (SewbdO) Salmonella enterica subsp. salamae serovar, respectively. Further, we optimized expression...

2′-fucosyllactose (2′-FL), one of the simplest but most abundant oligosaccharides in human milk, has been demonstrated to have many positive benefits for healthy development newborns. However, high-cost production and limited availability restrict its widespread use infant nutrition further research on potential functions. In this study, basis previous achievements, we developed a powerful cell factory by using lacZ -mutant Escherichia coli C41 (DE3)ΔZ ulteriorly increase 2′-FL feeding...

To rapidly type the fengycin homologues produced by Bacillus subtilis strains with electrospray ionization/collision-induced dissociation (ESI/CID) mass spectrometry.Fengycin JA were analysed. When each homologue was subjected to ESI/CID analysis, ions representing characteristic fragmentations detected. These can help identify homologues; even of same nominal be discriminated their spectra. Based on CID results, correctly assigned.ESI/CID leads rapid detection and structural...

The enzymatic hydrolysis of food waste by commercially available enzymes and the subsequent ethanol fermentation hydrolysates Saccharomyces cerecisiae H058 were studied in this work. optimum batch conditions found to be saccharification pH 4.5, temperature 55!, glucoamylase concentration 120 u/g, α-amylase 10 solid-liquid ratio 1: 0.75 (w/w). Fed process was started with a 1 (w/w), solid added at time lapse 2 h get final 0.5 After 4 reaction, reducing sugar reached 194.43 g/L digestibility...

Abstract BACKGROUND: A novel bacterial strain, Gulosibacter sp. YZ4, has been isolated from activated sludge. Its application potential for phenol biodegradation not yet reported, therefore, in this study, tests using strain YZ4 were executed under different conditions. RESULTS: The was identified as a new member of the genus and nominated YZ4. Phenol showed that could thoroughly biodegrade 1000 mg L −1 across wide temperature range 10 to 42 °C pH 5 11. Degradation inhibited by coexistence p...

Levilactobacillus brevis strains can be isolated from traditional Chinese pickles and used as the starter cultures to improve nutritional profiles of fermented juices. Three L. (LBG-29, LBG-24, LBD−14) that produce high levels gamma-aminobutyric acid (GABA; >300 mg/L) were pickles. The showed tolerance low pH bile salts exhibited safety in vitro. Litchi juice was using each strain at 37 °C for 48 h. litchi determined a good substrate fermentation process enhanced its functional profile....

The probiotic Weizmannia coagulans (W. coagulans) BC2000 can increase the abundance of intestinal transforming ellagic acid (EA) bacteria and inhibit metabolic disorders caused by hyperlipidemia activating liver autophagy. This study aimed to investigate inhibitory effects W. EA on hyperlipidemia-induced cholesterol metabolism disorders. C57BL/6J mice (n = 10 in each group) were fed a low-fat diet, high-fat diet (HFD), HFD supplemented with EA, BC77, BC2000. supplementation prevented...

ABSTRACT 3-fucosyllactose (3-FL) and 2'-fucosyllactose (2'-FL), are two important fucosylated oligosaccharides in human milk. Extensive studies on 2'-FL enabled its official approval for use infant formula. However, development of 3-FL has been somewhat sluggish due to low content milk poor yield enlarged production. Here, an α-1,3-fucosyltransferase mutant was introduced into engineered Escherichia coli (E. coli) capable producing GDP-L-fucose, leading a promising titer 5.0-L bioreactor. To...

(1) Background: Ellagic acid (EA) acts as a product of gut microbiota transformation to prevent insulin resistance, which is limited by high-fat diet (HFD)-induced dysbiosis. The aim this study was investigate the synergistic effects and mechanisms supplementation with probiotic Weizmannia coagulans (W. coagulans) on prevention resistance EA; (2) Methods: C57BL/6J mice were divided into five groups (n = 10/group): low-fat-diet group, high-fat-diet EA intervention + W. BC77 BC2000 group; (3)...

We developed a highly efficient magnetic relaxation switch (MRS) system based on poly(vinyl alcohol) functionalized nanomagnetic iron oxide (PVA@NMIO) particles for the detection of boric acid or borate ester (BA/BE). It was found that addition BA/BE induced aggregation PVA@NMIO particles, resulting in measurable change T2 time resonance measurements. The main mechanism proposed electron-deficient boron atoms caused through covalent binding to hydroxyl groups PVA. This novel displayed...

The objective of this study was to investigate the stability and degradation kinetics N-acetylneuraminic acid (Neu5Ac). pH solution strongly influenced Neu5Ac, which more stable at neutral low temperatures. Here, we provide detailed information on Neu5Ac different values (1.0, 2.0, 11.0 12.0) temperatures (60, 70, 80 90 °C). under acidic conditions (pH 1.0–2.0) is highly pertinent for hydrolysis polysialic acid. alkaline deacetylation were also studied. 3.0–10.0, even high temperature, but...

In this study, CS-NeuAc hydrogel was prepared from chitosan (CS) and N-acetylneuraminic acid (NeuAc) using cyclic freeze/thaw under 1-ethyl-3-(3-dimethylaminopropyl)-carbodiiminde/N-hydroxysuccinimide catalysis. Fourier transformed infrared spectroscopy, X-ray diffraction, scanning electron microscopy analyses confirmed the successful synthesis of hydrogel. hydrogels were found to be equipped with suitable swelling behaviour, porosity, water evaporation rate, degradation good mechanical...

Among the human milk oligosaccharides (HMOs), one of most abundant and has great benefits for health is 3′-sialyllactose (3′-SL). Given its important physiological functions lack cost-effective production processes, we constructed an in vitro multi-enzymatic cofactor recycling system biosynthesis 3′-SL from a low-cost substrate. First, biosynthetic pathway increased solubility cytidine monophosphate kinase (CMK) with chaperones. We subsequently identified that β-galactosidase (lacZ) affects...

C-NeuAc/OHEC hydrogels were prepared from carboxymethyl chitosan/N-acetylneuraminic acid (C-NeuAc) and oxidised hydroxyethyl cellulose (OHEC), used as novel vehicles for the co-encapsulation of Pediococcus pentosaceus RQ-1 isomaltose-oligosaccharide (IMO). The thermal stability, rheological behaviour, swelling kinetics, porosity, pH sensitivity, degradability evaluated. most stable suitable IMO when C-NeuAc:OHEC = 5:4. In presence IMO, encapsulation efficiency hydrogel was optimal in range...

2'-Fucosyllactose (2'-FL) is one of the most important oligosaccharides in human milk. In this study, a glucose–glycerol mixed carbon source and deletion genes arcA iclR were attempted for first time engineered Escherichia coli to improve 2'-FL production. The highest titre 5.04 g/L, almost 1.4 times higher than that control strain, was obtained mutant strain using shake-flask culture. addition, showed significantly reduced accumulation acetate. However, double arcA/iclR provided no benefit...

Fern root (FR) was used in the biofuel production for first time. However, fermentation of enzymatic hydrolysate FR starch (EHFS) directly to butanol by Clostridium acetobutylicum CGMCC1.0134 resulted a low and yield with high content residual. After adding yeast extract (YE) solution (with 3 g/L final concentration broth) into EHFS, production, productivity, were raised 174%, 250%, 183%, respectively. Monitoring changes free amino acid broth indicated that aspartic families serine...