A5012105750- Magnetic confinement fusion research
- Infant Nutrition and Health
- Fusion materials and technologies
- Digestive system and related health
- Particle accelerators and beam dynamics
- Superconducting Materials and Applications
- Glycosylation and Glycoproteins Research
- Biofuel production and bioconversion
- Probiotics and Fermented Foods
- Pharmaceutical and Antibiotic Environmental Impacts
- SARS-CoV-2 detection and testing
- Enzyme Production and Characterization
- Food composition and properties
- Antibiotic Resistance in Bacteria
- Biosensors and Analytical Detection
- Biodiesel Production and Applications
- Advanced biosensing and bioanalysis techniques
- Microbial Metabolic Engineering and Bioproduction
- Pediatric Hepatobiliary Diseases and Treatments
- Ionosphere and magnetosphere dynamics
- Microbial Metabolites in Food Biotechnology
- Biochemical and Molecular Research
- Heme Oxygenase-1 and Carbon Monoxide
- Nanoplatforms for cancer theranostics
- Gear and Bearing Dynamics Analysis
Hefei Institutes of Physical Science
2018-2025
Institute of Plasma Physics
2015-2025
Chinese Academy of Sciences
2015-2025
Shenyang University
2024
Institute of Applied Ecology
2024
Changchun Observatory
2024
Wenzhou University
2024
Sichuan University
2020-2023
East China University of Science and Technology
2023
Technical Institute of Physics and Chemistry
2022
Lacto-
Abstract At present, atmospheric and room‐temperature plasma (ARTP) is regarded as a new powerful mutagenesis technology with the advantages of environment‐friendliness, operation under mild conditions, fast speed. Compared traditional strategies, ARTP used mainly to change structure microbial DNA, enzymes, proteins through series physical, chemical, electromagnetic effects organisms, leading nucleotide breakage, conversion or inversion, causing various DNA damages, so screen out mutants...
Lacto-N-neotetraose (LNnT) and lacto-N-tetraose (LNT) are important oligosaccharides found in breast milk commonly used as nutritional supplements infant formula. We metabolic engineering techniques to optimize the modified Escherichia coli BL21 star (DE3) strain for efficient synthesis of LNnT LNT using β-1,4-galactosyltransferase (HpgalT) from Helicobacter pylori β-1,3-galactosyltransferase (SewbdO) Salmonella enterica subsp. salamae serovar, respectively. Further, we optimized expression...
Reductions in H content and particle recycling are important for the improvement of ion cyclotron range frequency (ICRF) minority heating efficiency enhancement plasma performance EAST superconducting tokamak. During recent years several techniques surface conditioning such as baking, glow discharge cleaning/ICRF cleaning, coatings, boronization, siliconization lithium coating, have all been attempted order to reduce H/(H+D) ratio EAST. Even though boronization were both reasonably effective...
A 4.6 GHz lower-hybrid current drive (LHCD) system has been firstly commissioned in EAST the 2014 campaign. The first LHCD results with show that LHW can be coupled to plasma a low reflection coefficient, and rotation, modify profile, heat effectively. By means of configuration optimization local gas puffing near antenna, good LHW–plasma coupling coefficient less than 5% is obtained. maximum power up 3.5 MW. (CD) efficiency 1.1 × 1019 m−2 W−1 central electron temperature above 4 keV,...
2′-fucosyllactose (2′-FL), one of the simplest but most abundant oligosaccharides in human milk, has been demonstrated to have many positive benefits for healthy development newborns. However, high-cost production and limited availability restrict its widespread use infant nutrition further research on potential functions. In this study, basis previous achievements, we developed a powerful cell factory by using lacZ -mutant Escherichia coli C41 (DE3)ΔZ ulteriorly increase 2′-FL feeding...
N-acetylneuraminic acid (NANA) is a key ingredient in bird’s nest, as well health products and infant formulas. However, the absence of suitable reference materials (RMs) makes it challenging to ensure quality these products. Currently, NANA primarily extracted from but this process costly difficult scale up. In study, we successfully prepared large-scale yield RM fermentation products, which increases reduces its cost. IR, MS, NMR methods were used for qualitative analysis RM, HPLC was...
3-Fucosyllactose (3-FL), an essential component of human milk oligosaccharides, is crucial for infant health. However, the extraction 3-FL from presents a significant challenge. In this study, E. coli BL21 Star (DE3) was engineered biosynthesis, and gene combinations metabolic pathways were optimized to obtain high-yield production strain. First, module integrated into coli. Second, efficient alpha-1,3-fucosyltransferase screened. Then, different integration sites used optimize precursor...
This study presents an engineered strain of Escherichia coli specifically designed to enhance the production l-fucose while minimizing residues 2′-fucosyllactose. The optimization strategies employed include selection key enzymes, gene copy numbers, and fermentation using mixed carbon sources. metabolic flux was directed toward synthesis by integrating preferred 1,2-fucosyltransferase α-l-fucosidase into genome. Furthermore, numbers were optimized enzyme expression, thereby increasing...
Ectoine is an important natural macromolecule protector that helps extremophiles maintain cellular stability and function under high-salinity conditions. Recently, the development of microbial strains for high-level ectoine production has become attractive research direction. In this study, we constructed efficient plasmid-free ectoine-producing strain. We modified 5′-untranslated region ectABC gene cluster from Halomonas elongate to fine-tune expression genes ectA, ectB, ectC. Furthermore,...
The active feedback control of radiated power to prevent divertor target plates overheating during long-pulse operation has been developed and implemented on EAST. radiation algorithm, with impurity seeding via a supersonic molecular beam injection (SMBI) system, shown great success in both reliability stability. By sequence short neon (Ne) pulses the SMBI from outer mid-plane, bulk plasma can be well controlled, duration radiative (feedforward feedback) is 4.5 s discharge 10 s. Reliable...
Levilactobacillus brevis strains can be isolated from traditional Chinese pickles and used as the starter cultures to improve nutritional profiles of fermented juices. Three L. (LBG-29, LBG-24, LBD−14) that produce high levels gamma-aminobutyric acid (GABA; >300 mg/L) were pickles. The showed tolerance low pH bile salts exhibited safety in vitro. Litchi juice was using each strain at 37 °C for 48 h. litchi determined a good substrate fermentation process enhanced its functional profile....
Abstract Background Radiation-induced skin injury remains a serious concern during radiotherapy. Cu/Zn-superoxide dismutase (Cu/Zn-SOD, SOD 1 ) is conserved enzyme for scavenging superoxide radical in cells. Because of the integrity cell membranes, exogenous molecule not able to be incorporated into cells, which limited application natural . The aim this study was evaluate protective role HIV-TAT protein transduction domain mediated (TAT-SOD against ionizing radiation. Methods recombinant...
The probiotic Weizmannia coagulans (W. coagulans) BC2000 can increase the abundance of intestinal transforming ellagic acid (EA) bacteria and inhibit metabolic disorders caused by hyperlipidemia activating liver autophagy. This study aimed to investigate inhibitory effects W. EA on hyperlipidemia-induced cholesterol metabolism disorders. C57BL/6J mice (n = 10 in each group) were fed a low-fat diet, high-fat diet (HFD), HFD supplemented with EA, BC77, BC2000. supplementation prevented...
ABSTRACT 3-fucosyllactose (3-FL) and 2'-fucosyllactose (2'-FL), are two important fucosylated oligosaccharides in human milk. Extensive studies on 2'-FL enabled its official approval for use infant formula. However, development of 3-FL has been somewhat sluggish due to low content milk poor yield enlarged production. Here, an α-1,3-fucosyltransferase mutant was introduced into engineered Escherichia coli (E. coli) capable producing GDP-L-fucose, leading a promising titer 5.0-L bioreactor. To...
Wastewater-based epidemiology has become a powerful surveillance tool for monitoring the pandemic of COVID-19. Although it is promising to quantitatively correlate SARS-CoV-2 RNA concentration in wastewater with incidence community infection, there still no consensus on whether viral nucleic acid sewage should be normalized against abundance endogenous biomarkers and which biomarker used as reference normalization. Here, several candidate normalization signal municipal were evaluated. The...
3'-Sialyllactose (3'-SL), a key component of human milk oligosaccharides, provides significant health benefits and immune modulation, is increasingly used in infant formula dietary supplements. This study presents novel approach for the efficient biosynthesis 3'-SL using
(1) Background: Ellagic acid (EA) acts as a product of gut microbiota transformation to prevent insulin resistance, which is limited by high-fat diet (HFD)-induced dysbiosis. The aim this study was investigate the synergistic effects and mechanisms supplementation with probiotic Weizmannia coagulans (W. coagulans) on prevention resistance EA; (2) Methods: C57BL/6J mice were divided into five groups (n = 10/group): low-fat-diet group, high-fat-diet EA intervention + W. BC77 BC2000 group; (3)...
Bacteria-caused wound infection greatly threatens human health, thus developing an efficient and safe antibacterial agent without drug resistance is still a great challenge. Herein, confined vulcanization strategy proposed to construct copper sulfides-loaded dual-mesoporous silica nanospheres (CuxSy@DMSNs) with various crystal phases for reactive oxygen species (ROS)-mediated photothermal application. With the pore confinement of DMSNs, sulfides including CuS, Cu9S5 Cu1.96S can be easily...
Lower hybrid current drive (LHCD) is an effective tool to achieve high confinement (H-mode) plasma in EAST. To utilize LHCD for accessing H-mode plasma, efforts have been made improve LHW (lower wave)-plasma coupling and capability at density. Improved LHW-plasma by means of local gas puffing from the electron side routinely used during EAST operation with LHCD. High density experiments suggest that low recycling LH frequency are preferred density, consistent previous results other machines....