A5030928461- Planarian Biology and Electrostimulation
- Adenosine and Purinergic Signaling
- Marine Ecology and Invasive Species
- Neuroscience and Neuropharmacology Research
- Cephalopods and Marine Biology
- Aquatic Invertebrate Ecology and Behavior
- Parasite Biology and Host Interactions
- Environmental Toxicology and Ecotoxicology
- Receptor Mechanisms and Signaling
- Genetic Neurodegenerative Diseases
- Neurotransmitter Receptor Influence on Behavior
- Autophagy in Disease and Therapy
- Insect and Arachnid Ecology and Behavior
- Parkinson's Disease Mechanisms and Treatments
- Marine Invertebrate Physiology and Ecology
- Protist diversity and phylogeny
- Pharmacological Receptor Mechanisms and Effects
- Marine Biology and Environmental Chemistry
- Reproductive biology and impacts on aquatic species
- Connexins and lens biology
- Mitochondrial Function and Pathology
- Neurological disorders and treatments
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Nanoparticle-Based Drug Delivery
University of Pisa
2014-2024
National Interuniversity Consortium of Materials Science and Technology
2021
Ospedale Cisanello
2004
University of Milan
2002
National Institute of Diabetes and Digestive and Kidney Diseases
2002
National Institutes of Health
2002
Polo d’Innovazione di Genomica
1999
Small extracellular vesicles have been intensively studied as a source of biomarkers in neurodegenerative disorders. The possibility to isolate neuron-derived small (NDsEV) from blood represents potential window into brain pathological processes. To date, the absence sensitive NDsEV isolation and full proteome characterization methods has meant their protein content underexplored, particularly for individual patients. Here, we report rapid method based on an immunoplate covalently coated...
Vascular calcification is a pathological chronic condition characterized by calcium crystal deposition in the vessel wall and recurring event atherosclerosis, kidney disease, diabetes. The lack of effective therapeutic treatments opened research to natural products, which have shown promising potential inhibiting process different experimental models. This study investigated anti-calcifying effects Quercetin Berberine extracts on vascular smooth muscle cells (VSMCs) treated with an inorganic...
Background/Objectives: Under physiological conditions, vascular smooth muscle cells (VSMCs) are in a quiescent contractile state, but under pathological such as atherosclerosis, they change their phenotype to synthetic, characterized by increased proliferation, migration, and production of extracellular matrix. Furthermore, VSMCs can undergo calcification, switching an osteoblast-like phenotype, contributing plaque instability. Methods: In this study, we analyzed the phenotypic changes...
We report the presence of two Pax6-related genes, Pax6A and Pax6B, which are highly conserved in planarian species Dugesia japonica Girardia tigrina (Platyhelminthes, Tricladida). is more similar to other Pax6 proteins than most divergent described so far. The homologs do not show any clear orthology Drosophila duplicated eyeless twin eyeless, suggests an independent duplication a triclad or platyhelminth ancestor. expressed central nervous system intact planarians, labeling subset cells...
Glioblastoma (GBM) cells feature mitochondrial alterations, which are documented and quantified in the present study, by using ultrastructural morphometry. Mitochondrial impairment, roughly occurs half of organelles, is shown to be related mTOR overexpression autophagy suppression. The novelty study consists detailing an mTOR-dependent mitophagy occlusion, along with suppression fission. These phenomena contribute explain increase altered mitochondria reported here. Administration inhibitor...
A<sub>3</sub> adenosine receptor activation has been previously demonstrated to result in both neuroprotective and neurodegenerative effects, depending upon specific pathophysiological conditions. This dual effect may depend on regulation mechanisms that are able change availability and/or function. In the present study, we investigated desensitization, internalization, down-regulation of native receptors human astrocytoma cells after exposure agonist...
Abstract Recent studies demonstrated that methamphetamine (METH) produces intracellular bodies which are reminiscent of those occurring during degenerative disorders. In vivo demonstrate the occurrence these morphological alterations both in dopamine (DA) neurons substantia nigra and striatal cells. These consist neuronal staining for a variety antigens belonging to ubiquitin–proteasome pathway. The formation PC12 cells depends on presence endogenous DA. present study, we analyze mechanisms...
Mutations in the PTEN-induced putative kinase1 (PINK1) represent second most frequent cause of autosomal recessive Parkinson's disease. The PINK1 protein mainly localizes to mitochondria and interacts with a variety proteins, including pro-autophagy beclin1 ubiquitin-ligase parkin. Upon stress conditions, is known recruit parkin at surface dysfunctional activate mitophagy cascade. Aim this study was use simple highly reproducible catecholamine cell model transmission electron microscopy...
Amyotrophic lateral sclerosis (ALS) is characterized by massive loss of motor neurons. Data from ALS patients and experimental models indicate that mitochondria are severely damaged within dying or spared Nonetheless, recent data mitochondrial preservation, although preventing neuron loss, fails to prolong lifespan. On the other hand, damage axons plays a pivotal role in determining both lethality disease course. Thus, present article each compartment (cell body, central, peripheral axons)...
A(3) adenosine receptors have been proposed to play an important role in the pathophysiology of cerebral ischemia with a regimen-dependent nature therapeutic effects probably related receptor desensitization and down-regulation. Here we studied agonist-induced internalization human transfected Chinese hamster ovary cells, then evaluated relationship between signal resensitization. Binding N(6)-(4-amino-3-[(125)I]iodobenzyl)adenosine-5'-N-methyluronamide membranes from cells stably showed...
Exposure to loud noise is a major environmental threat public health. Loud exposure, apart from affecting the inner ear, deleterious for cardiovascular, endocrine and nervous systems it associated with neuropsychiatric disorders. In this study we investigated DNA, neurotransmitters immune-histochemical alterations induced by exposure in three brain areas (cerebellum, hippocampus, striatum) of Wistar rats. Rats were exposed (100 dBA) 12 h. The effects on DNA integrity all evaluated using...
Abstract: The 6‐hydroxydopamine (6‐OHDA) model of Parkinson's disease in the rat represents a fundamental tool for investigating pathophysiology dopamine denervation. Nevertheless, 6‐OHDA can induce also noradrenergic lesions; therefore desmethylimipramine (DMI) is co‐administrated as selective inhibitor reuptake to protect noradrenaline (NA) fibers neighboring DA neurons and/or axons. neurotoxin must be microinfused selectively into substantia nigra pars compacta (SNpc) or medial forebrain...
// Michela Ferrucci 1,* , Francesca Biagioni 2,* Paola Lenzi 1 Stefano Gambardella 2 Rosangela Ferese Maria Teresa Calierno Alessandra Falleni 3 Alfonso Grimaldi 4 Alessandro Frati Vincenzo Esposito 2,4 Cristina Limatola Francesco Fornai 1,2 Department of Translational Research and New Technologies in Medicine Surgery, Human Anatomy, University Pisa, Italy Istituto di Ricovero e Cura a Carattere Scientifico, Neuromed, Pozzilli, Isernia, Clinical Experimental Medicine, Physiology...
Vascular calcification (VC) is a cardiovascular disease characterized by calcium salt deposition in vascular smooth muscle cells (VSMCs). Standard vitro models used VC investigations are based on VSMC monocultures under static conditions. Although these platforms easy to use, the absence of interactions between different cell types and dynamic conditions makes insufficient study key aspects pathophysiology. The present aimed develop endothelial cell-VSMC co-culture that better mimics vivo...
Cytokines produced by immune system cells infiltrating pancreatic islets are candidate mediators of islet beta-cell destruction in autoimmune insulin-dependent diabetes mellitus. After 72 h exposure human to a cytotoxic cytokine combination interleukin 1 beta (50 U/ml), tumor necrosis factor alpha (1,000 and interferon gamma an increase cell death vs. control was demonstrated TUNEL detection ELISA method. Islet associated with apoptosis mitochondrial swelling as evidenced electron...
OBJECTIVE: Peripheral benzodiazepine receptors (PBRs) are part of the mitochondrial permeability transition pore, and their activation may induce cell death. PBRs expressed in human pancreatic islets, cytokine-induced damage is accompanied by changes properties. We hypothesized that can have a role islet physiopathology, evaluated effects prolonged exposure to two specific PBR ligands, PK11195 Ro5-4864 on function survival isolated islets. DESIGN: Isolated islets were prepared from pancreas...