We developed a photoacoustic imaging system that has real-time capability with optical resolution. The is capable of scanning at 20 Hz over 9 mm range and up to 40 1 range. A focused laser beam provides lateral resolution 3.4 μm as measured in an optically nonscattering medium. Flows micrometer-sized carbon particles or whole blood silicone tube individual red cells (RBCs) mouse ear capillaries were also imaged real time, demonstrating the image highly dynamic processes vivo micrometer-scale

We present label-free functional photoacoustic imaging of the ocular microvasculature in living animals. The anterior segment an adult mouse was imaged with a laser exposure level well within American National Standards Institute safety standards. Individual red blood cells traveling along iris capillaries were clearly resolved, and hemoglobin oxygen saturation spectrally. believe that has potential to advance diagnosis treatment diseases humans.

Recently developed optical-resolution photoacoustic microscopy (OR-PAM), which is based on the detection of optical absorption contrast, complementary to other modalities such as confocal microscopy, coherence tomography, and multiphoton microscopy. A hybrid optical-mechanical scanning configuration increases imaging speed OR-PAM significantly, facilitating many demanding biomedical applications. With a high-pulse-repetition-rate laser, hybrid-scanning can acquire one-dimensional...

Optical-resolution photoacoustic microscopy (OR-PAM) has become a major experimental tool of tomography, with unique imaging capabilities for various biological applications. However, conventional systems are all table-top embodiments, which preclude their use in internal organs. In this study, by applying the OR-PAM concept to our recently developed endoscopic technique, called endoscopy (PAE), we created an optical-resolution endomicroscopy (OR-PAEM) system, enables organ much finer...

Colorectal cancer is the second leading cause of death in United States. Significant limitations screening and surveillance modalities continue to hamper early detection primary cancers or recurrences after therapy. In this study, we describe a new registered ultrasound (US) acoustic-resolution photoacoustic microscopy (AR-PAM) system report its initial testing ex vivo human colorectal tissue. A total 8 specimens were imaged, which included 2 polyps, 4 malignant colon cancers, treated...

We present evidence that rice tungro spherical virus (RTSV) has a genome of polyadenylated single-stranded RNA about 10 kb whereas bacilliform (RTBV) contains double-stranded circular DNA. RTBV DNA been mapped and shown to have two discontinuities, one in each strand, at specific sites; it thus resembles the caulimo-viruses. Gel electrophoresis RTSV preparations revealed protein bands (M r 35K 26K). yielded major 37K 33K together with several minor species higher lower M which react antiviral serum.

Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and stationary ultrasonic transducer was built. Four-dimensional vivo imaging microcirculation mouse skin achieved at 18 three-dimensional volumes per second repeated two-dimensional (2D) raster scans 100 by 50 points. The corresponding 2D B-scan (50 A-lines) frame 1800 Hz, the...

Abstract Non-invasive optical imaging of neuronal voltage response signals in live brains is constrained depth by the diffusion limit, which due primarily to scattering brain tissues. Although photoacoustic tomography breaks this limit exciting targets with diffused photons and detecting resulting acoustic responses, it has not been demonstrated as a modality for responses. In communication, we report first demonstration both vitro HEK-293 cell cultures vivo mouse surfaces. Using...

The invention of green fluorescent protein and other molecular probes has promoted applications confocal two-photon fluorescence microscopy in biology medicine. However, exogenous contrast agents may affect cellular structure function, cannot image nonfluorescent chromophores. We overcome this limitation by integrating optical-resolution photoacoustic into a modern Olympus IX81 confocal, two-photon, microscope setup to provide complementary, label-free, optical absorption contrast....

Ovarian cancer is the leading cause of death among gynecological cancers, but poorly amenable to preoperative diagnosis. In this study, we investigate feasibility "optical biopsy," using high-optical-resolution photoacoustic microscopy (OR-PAM) quantify microvasculature ovarian and fallopian tube tissue. The technique demonstrated excised human ovary specimens imaged immediately after surgery. Quantitative parameters are derived Amira software. include three-dimensional vascular segment...

Abstract Colorectal cancer diagnosis currently relies on histological detection of endoluminal neoplasia in biopsy specimens. However, clinical visual endoscopy provides no quantitative subsurface information. In this ex vivo study nine fresh human colon specimens, we report the first use quantified scattering coefficient maps acquired by swept-source optical coherence tomography to reveal abnormities. We generate with a novel wavelet-based-curve-fitting method that significantly improved...

Abstract Ovarian cancer is a heterogeneous disease at the molecular and histologic level. Optical coherence tomography (OCT) able to map ovarian tissue optical properties heterogeneity, which has been proposed as feature aid in diagnosis of cancer. In this manuscript, depth‐resolved en face scattering maps malignant ovaries, benign fallopian tubes obtained from 20 patients are provided visualize heterogeneity tissues. Six features extracted histograms maps. All statistically distinguish...

Photoacoustic microscopy (PAM) utilizes short laser pulses to deposit energy into light absorbers and sensitively detects the ultrasonic waves generate in response. PAM directly renders a three-dimensional spatial distribution of sub-surface optical absorbers. Unlike other imaging technologies, features label-free absorption contrast excellent depths. Standard dental instruments are limited X-ray CCD cameras. Subsurface is difficult due highly-scattering enamel dentin tissue. Thus, very few...

Optical imaging of responses in fluorescently labeled neurons has progressed significantly recent years. However, there is still a need to monitor neural activities at divergent spatial scales and depths beyond the optical diffusion limit.

Both iris fluorescein angiography (IFA) and indocyanine green (ICGA) provide ophthalmologists imaging tools in studying the microvasculature structure hemodynamics of anterior segment eye normal diseased status. However, a non-invasive, endogenous modality is preferable for monitoring microvasculature. We investigated vivo, label-free ocular with photo-acoustic microscopy (PAM) mouse eyes. demonstrated unique advantage contrast that not available both IFA ICGA. The laser radiation was...

We previously demonstrated that multimodal microscopy combining photoacoustic and optical coherence tomography can provide comprehensive insight into biological tissue at μm-level resolution by exploiting both absorption scattering contrasts. Recently, we have developed a second-generation integrated optical-coherence microscope, which potentially be adapted for clinical applications. In this new system, perform imaging simultaneously speed of 5,000 A-lines per second with real-time...

Neural scientists can benefit greatly from imaging tools that penetrate thick brain tissue. Compared with traditional optical microscopy methods, photoacoustic beat the diffusion limit and achieve such deep tissue high spatial resolution. In this study, we used an optical-resolution microscope to image odor-evoked neuronal activities in a drosophila model. Drosophila neurons stably express GCaMP5G, calcium-sensitive fluorescent protein whose absorption coefficient changes calcium influx...

Intravital microscopy techniques have become increasingly important in biomedical research because they can provide unique microscopic views of various biological or disease developmental processes situ.Here we present an opticalresolution photoacoustic endomicroscopy (OR-PAEM) system that visualizes internal organs with a much finer resolution than conventional acoustic-resolution endoscopy systems.By combining gradient index (GRIN) lens-based optical focusing and ultrasonic ring...

Optical imaging of brain voltage signals is significantly limited in depth due to optical scattering and the absorptive property tissue. Photoacoustic (PA) promises break this hard limit by utilizing both ballistic diffused photons. To demonstrate feasibility PA, we used an vivo mouse model. The cortex tissue was stained with dipicrylamine dye, electrically stimulated, imaged a customized dual-isosbestic-wavelength PA microscope (DIW-PAM). DIW-PAM separates voltage-induced from blood-induced...

Optical-resolution photoacoustic microscopy (OR-PAM) provides superb optical absorption contrast for red blood cells (RBCs), which makes it ideal in vivo microvasculature imaging. In comparison, coherence tomography (OCT), widely used tissue microstructure imaging, high scattering contrast. The two mechanisms are highly complementary. this work, we combined OR-PAM and OCT into a single, dual-modality imaging instrument mouse eye We demonstrated of the anterior segment eyes with laser pulse...

Colorectal cancer is the second leading cause of death in United States. According to American Cancer society, overall lifetime risk developing colorectal about 4.7% for men and 4.4% women. We have developed a rigid, endoscopic photoacoustic microscopy (PAM) probe imaging vivo human cancers. In order accommodate colon sections with different size (typically from 50 70mm), our 10mm diameter rigid uses an off-optical-axis, external mechanical scanning mechanism speed 35deg/s instead...

Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and stationary ultrasonic transducer was built. Four-dimensional vivo imaging microcirculation mouse skin achieved at 18 three-dimensional volumes per second repeated two-dimensional raster scans 100 by 50 points. The corresponding twodimensional B-scan (50 A-lines) frame 1800 Hz, the...