A5048345046- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Thermography and Photoacoustic Techniques
- SARS-CoV-2 detection and testing
- Nanoplatforms for cancer theranostics
- Optical Imaging and Spectroscopy Techniques
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Microfluidic and Bio-sensing Technologies
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Photonic and Optical Devices
- Animal Genetics and Reproduction
- Bacterial Genetics and Biotechnology
- Advanced Frequency and Time Standards
- Plant Virus Research Studies
- Modular Robots and Swarm Intelligence
- Photovoltaic System Optimization Techniques
- Malaria Research and Control
- Solar Radiation and Photovoltaics
- Optical Network Technologies
Bar-Ilan University
2016-2025
Washington University in St. Louis
2010-2015
Optica
2010-2015
Tel Aviv University
2000-2011
Super-resolution microscopy techniques - capable of overcoming the diffraction limit light have opened new opportunities to explore subcellular structures and dynamics not resolvable in conventional far-field microscopy. However, relying on staining with exogenous fluorescent markers, these can sometimes introduce undesired artifacts image, mainly due large tagging agent sizes insufficient or variable labeling densities. By contrast, use endogenous pigments allows imaging intrinsic...
Optical-resolution photoacoustic microscopy (OR-PAM) has become a major experimental tool of tomography, with unique imaging capabilities for various biological applications. However, conventional systems are all table-top embodiments, which preclude their use in internal organs. In this study, by applying the OR-PAM concept to our recently developed endoscopic technique, called endoscopy (PAE), we created an optical-resolution endomicroscopy (OR-PAEM) system, enables organ much finer...
Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and stationary ultrasonic transducer was built. Four-dimensional vivo imaging microcirculation mouse skin achieved at 18 three-dimensional volumes per second repeated two-dimensional (2D) raster scans 100 by 50 points. The corresponding 2D B-scan (50 A-lines) frame 1800 Hz, the...
Photoacoustic tomography (PAT) is a hybrid imaging technique that has broad preclinical and clinical applications. Based on the photoacoustic effect, PAT directly measures specific optical absorption, which product of tissue-intrinsic absorption coefficient local fluence. Therefore, quantitative PAT, such as absolute oxygen saturation (sO2) quantification, requires knowledge fluence, can only be estimated through invasive measurements or sophisticated modeling light transportation. In this...
Dengue virus (DENV) infection is a significant global health concern, causing severe morbidity and mortality. While many cases present as mild febrile illness, some progress to life-threatening dengue (SD). Early intervention essential improve outcomes, but current predictive methods lack specificity, burdening healthcare systems in endemic regions. Circulating long non-coding RNAs (lncRNAs) have emerged stable promising biomarkers. This study explored the use of lncRNAs markers for SD....
Picosecond absorption relaxation-central to many disciplines-is typically measured by ultrafast (femtosecond or picosecond) pump-probe techniques, which however are restricted optically thin and weakly scattering materials require artificial sample preparation. Here, we developed a reflection-mode relaxation photoacoustic microscope based on nanosecond laser picosecond times. The times of oxygenated deoxygenated hemoglobin molecules, both possessing extremely low fluorescence quantum yields,...
Recently, we developed a reflection-mode relaxation photoacoustic microscope, based on saturation intensity, to measure picosecond times using nanosecond laser. Here, the different of oxygenated and deoxygenated hemoglobin molecules, both possessing extremely low fluorescence quantum yields, oxygen was quantified in vivo with single-wavelength microscopy. All previous functional microscopy measurements required imaging multiple-laser-wavelength quantify saturation. Eliminating need for...
As light intensity increases in photoacoustic imaging, the saturation of optical absorption and temperature dependence thermal expansion coefficient result a measurable nonlinear (PA) signal on excitation pulse fluence. Here, under controlled conditions, we investigate intensity-dependent signals from oxygenated deoxygenated hemoglobin at varied wavelengths molecular concentrations. The wavelength concentration dependencies PA spectrum are found to be significantly greater than hemoglobin....
Abstract In fluorescence‐based assays, usually a target molecule is captured using probe conjugated to capture surface, and then detected second fluorescently labeled probe. One of the most common surfaces magnetic bead. However, beads exhibit strong autofluorescence, which often overlaps with emission reporter fluorescent dyes limits analytical performance assay. Here, several widely used are photobleached their autofluorescence reduced 1% initial value. Their properties, including...
pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance regulation may result from or in serious illness. this paper, we report photoacoustic microscopy (PAM) commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) tissue phantoms. We demonstrated that PAM capable imaging absolute values at depths up to 2.0 mm, greater than possible with other forms optical microscopy.
Zika virus has created global alarm because it been associated with catastrophic fetal abnormalities, including microcephaly, spontaneous abortion, and intrauterine growth restriction. Current serological assays that detect antiviral antibodies suffer from low sensitivity high cross-reactivity among different flaviviruses. In this study, utilizing a novel magnetic modulation biosensing (MMB) system the nonstructural 1 protein, we show highly sensitive specific assays. We blindly tested 60...
A sensitive and rapid method for detecting fluorescent dyes at low concentrations in homogenous solution is experimentally demonstrated. Fluorescent-labeled DNA probes are detected by attaching magnetic beads applying alternating field gradient. This condenses the into a small detection volume eliminates scattering noise from synchronous detection. For concentration of 1 x 10(-13) M signal was 3.3 times higher than noise, thereby implying sensitivity 3 10(-14) M.
Identifying and investigating protein–DNA interactions, which play significant roles in many biological processes, is essential for basic clinical research. Current techniques identification of interactions are laborious, time-consuming, suffer from nonspecific binding limited sensitivity. To overcome these challenges assess we use a magnetic modulation biosensing (MMB) system. In MMB, one the interacting elements (protein or DNA) immobilized to beads, other coupled fluorescent molecule....
Improvements in the power level of sources near 1550 nm and efficiency waveguide frequency doublers enabled us to lock a frequency-doubled source directly 5S1/2→5D5/2 two-photon transitions 778 nm. We obtained sufficiently powerful second-harmonic signal, exceeding 2 mW, by doubling an external-cavity diode laser that was amplified erbium-doped fiber amplifier periodically poled LiNbO3 channel waveguide. Our experimental scheme can be used for realizing compact, high-performance standards...
Repetitive DNA sequences are abundant in the genome of most biological species. These naturally "preamplified", which makes them a preferential target for variety assays. Current methods to detect specific based on quantitative polymerase chain reaction (PCR), relies amplification by Taq and uses fluorescent resonance energy transfer (FRET)-based probe. Here, rapidly repetitive sequence, we combine highly sensitive magnetic modulation biosensing (MMB) system modified double-quenched...
Detection of biomarkers at low concentrations is essential for early diagnosis numerous diseases. In many sensitive assays, the target molecules are tagged using fluorescently labeled probes and captured magnetic beads. Magnetic beads facilitate washing separation steps, well suited automation, improve assay sensitivity. Current devices rely on quantifying by detecting fluorescence signal from individual Thus, to detect molecules, these require sophisticated optical detectors, making them...
Toxin-antitoxin (TA) systems are genetic modules that consist of a stable protein-toxin and an unstable antitoxin neutralizes the toxic effect. In type II TA systems, is protein inhibits toxin by direct binding. Type whose roles functions under intensive study, highly distributed among bacterial chromosomes. Here, we identified characterized novel system PrrT/A encoded in chromosome clinical isolate 39016 opportunistic pathogen Pseudomonas aeruginosa. We have shown exhibits classical...