A5060265645- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Ion channel regulation and function
- SARS-CoV-2 detection and testing
- Receptor Mechanisms and Signaling
- Cardiac electrophysiology and arrhythmias
- Advanced Biosensing Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Bacteriophages and microbial interactions
- Neural dynamics and brain function
- Photochromic and Fluorescence Chemistry
- Photoreceptor and optogenetics research
- Potassium and Related Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Viral gastroenteritis research and epidemiology
- Animal Genetics and Reproduction
- Protein Kinase Regulation and GTPase Signaling
- Molecular Biology Techniques and Applications
- Computational Drug Discovery Methods
- Ion Transport and Channel Regulation
- Identification and Quantification in Food
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
Bar-Ilan University
2019-2024
Quantitative BioSciences
2021
Merck & Co., Inc., Rahway, NJ, USA (United States)
2010-2017
Merck (Japan)
2005
University of Maryland, Baltimore
1994-1999
Russian Academy of Sciences
1992
The formation of functional synapses is a late milestone neuronal differentiation. establishment can be used to assess characteristics different cell lines. In the present study, we examined in vitro conditions that influence ability human neurons derived from NT2 line (NT2N neurons) establish synapses. morphologic, immunologic, and electrophysiologic these was examined. absence astrocytes, NT2N rarely formed their action potentials were weak uncommon. contrast, when plated on primary able...
Margulis, Michael and Cha-Min Tang. Temporal integration can readily switch between sublinear supralinear summation. J. Neurophysiol. 79: 2809–2813, 1998. summation at dendrites of cultured rat hippocampal neurons was examined as a function the interval separating two dendritic inputs. A novel method that relies on single-mode optical fibers to achieve rapid photorelease glutamate developed. Dendritic excitation achieved with this approach resembles associated miniature excitatory...
The introduction of parallel patch clamp instruments offers the promise moderate-throughput, high-fidelity voltage for drug screening assays. One such device, IonWorks™ HT (Molecular Devices, Sunnyvale, CA), was evaluated and compared to conventional human ethera- go-go-related gene (hERG) data an alternative functional screen based on rubidium flux. Data generated by IonWorks assays were determine if either offered superior predictive value clamp. Concentration–effect curves a panel known...
We report the synthesis, physicochemical characterization, and biological evaluation of a new caged glutamate,N-(o-nitromandelyl)oxycarbonyl-l-glutamic acid (Nmoc-Glu), that liberates free glutamate on photolysis. The low affinity certain receptors their rapid entry into desensitization have effectively prevented creation an ideal glutamate. In absence compound, Nmoc-Glu was designed to resist spontaneous hydrolysis while maintaining reasonable photorelease yield kinetics. Chemical...
In vitro screens using cellular preparations expressing human Ether-à-go-go related gene (hERG) potassium channels have become an intrinsic tool for evaluating cardiac liability of compounds during early preclinical stage development. Although hERG channel blocking effects are most reliably evaluated the low-throughput, manual patch clamp technique, methods and technologies that yield activity data in multiwell format required to address increased throughput requirements. cases, approaches...
A new subseries of ROMK inhibitors exemplified by 28 has been developed from the initial screening hit 1. The excellent selectivity for inhibition over related ion channels and pharmacokinetic properties across preclinical species support further evaluation as a mechanism diuretic. Robust pharmacodynamic effects in both SD rats dogs have demonstrated.
Repetitive DNA sequences are abundant in the genome of most biological species. These naturally "preamplified", which makes them a preferential target for variety assays. Current methods to detect specific based on quantitative polymerase chain reaction (PCR), relies amplification by Taq and uses fluorescent resonance energy transfer (FRET)-based probe. Here, rapidly repetitive sequence, we combine highly sensitive magnetic modulation biosensing (MMB) system modified double-quenched...
Detection of biomarkers at low concentrations is essential for early diagnosis numerous diseases. In many sensitive assays, the target molecules are tagged using fluorescently labeled probes and captured magnetic beads. Magnetic beads facilitate washing separation steps, well suited automation, improve assay sensitivity. Current devices rely on quantifying by detecting fluorescence signal from individual Thus, to detect molecules, these require sophisticated optical detectors, making them...
Rapid, highly sensitive, and high-throughput detection of biomarkers at low concentrations is invaluable for the early diagnosis various diseases. In many sensitive immunoassays, protocol time-consuming requires a complicated expensive system. Previously, we presented optical modulation biosensing (ht-OMB) system, which enables reading 96-well plate within 10 minutes. ht-OMB, to aggregate immobilize magnetic beads one spot, single cylindrical permanent magnet with sharp tip positioned under...
The COVID-19 pandemic highlighted the necessity of fast, sensitive, and efficient methods to test large populations for respiratory viruses. "gold standard" molecular assays detecting viruses, such as quantitative polymerase chain reaction (qPCR) reverse transcription qPCR (RT-qPCR), rely on invasive swab samples require time-consuming labor-intensive extraction processes. Moreover, turnaround time RT-qPCR-based is too lengthy rapid screening. Extraction-free saliva-based provide a...
Pro-arrhythmia by noncardiac drugs has become an important safety concern in the pharmaceutical industry. The most common underlying mechanism for induction of arrhythmias is off-target block native cardiac repolarizing current, I Kr. pore-forming subunit Kr encoded human ether-a-go-go related gene (hERG), and vitro measurements hERG activity a standard component drug evaluations. hERG/I channels are blocked wide array different chemical series; therefore, patients could be exposed to...
In vitro hERG blocking potency is measured in drug discovery as part of an integrated cardiovascular risk assessment. Typically, the concentrations producing 50% inhibition are protein-free saline solutions and compared with calculated free therapeutic vivo Cmax values to estimate a safety multiple. The free/unbound fraction believed responsible for activity. We tested validity this approach 12 compounds by determining potencies voltage clamp studies conducted absence presence 100% dialyzed...
In many sensitive assays, target molecules are tagged using fluorescently labeled probes and captured magnetic beads. Here, we introduce an optical modulation biosensing (OMB) system, which aggregates the beads into a small detection area separates signal from background noise by manipulating laser beam in out of cluster Using OMB system to detect human interleukin-8, demonstrated limit 0.02 ng/L 4-log dynamic range. Zika-positive healthy individuals’ serum samples, show that OMB-based Zika...
The COVID-19 pandemic has emphasized the inability of diagnostic laboratories' testing capacity to keep up with surging demand. primary reasons were lack reagents (e.g., viral transport media and nucleic acid extraction kits) low throughput gold-standard molecular detection method (RT-qPCR). While reagent shortages eventually resolved, limited RT-qPCR remains a bottleneck for high-throughput applications even today. Here, we introduce rapid saliva-based extraction-free assay, which utilizes...
Detection of biomarkers at low concentrations is essential for early diagnosis numerous diseases. In many sensitive assays, the target molecules are tagged using fluorescently labeled probes and captured magnetic beads. Current devices rely on quantifying by detecting fluorescent signal from individual Here, we propose a compact fluorescence-based magnetically aggregated biosensors (MAB) system. Using device to detect human Interleukin-8, demonstrated 0.1 ng/L limit detection 4-log dynamic...
A renal outer medullary potassium channel (ROMK, Kir1.1) is a putative drug target for novel class of diuretics with potential treating hypertension and heart failure. Our first disclosed clinical ROMK compound, 2 (MK-7145), demonstrated robust diuresis, natriuresis, blood pressure lowering in preclinical models, reduced urinary excretion compared to the standard care diuretics. However, projected short human half-life (∼5 h) that could necessitate more frequent than once day dosing. In...