A5030589826- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Acute Myeloid Leukemia Research
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Rheumatoid Arthritis Research and Therapies
- Cancer Research and Treatments
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Cutaneous lymphoproliferative disorders research
- Psoriasis: Treatment and Pathogenesis
- Neutropenia and Cancer Infections
- Vascular Tumors and Angiosarcomas
- Systemic Lupus Erythematosus Research
- Nerve injury and regeneration
- Childhood Cancer Survivors' Quality of Life
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Histone Deacetylase Inhibitors Research
- Adolescent and Pediatric Healthcare
- Mast cells and histamine
- HER2/EGFR in Cancer Research
University of Washington
2023-2025
Fred Hutch Cancer Center
2023-2025
VA Puget Sound Health Care System
2024
University of California, Davis
2013-2023
Stanford University
2001-2022
University of California, San Francisco
2018
University of California, Berkeley
2014-2016
Sacramento VA Medical Center
2014
University of California Davis Medical Center
2009-2014
Berkeley College
2014
The concept of idiotype vaccines against tumor-associated antigens (TAA) was tested in the DBA/2 L1210 lymphoma subline, L1210/GZL. Monoclonal antibodies a TAA that cross-reacts with envelope glycoprotein gp52 mammary tumor virus were used to make hybridoma anti-idiotype (Ab2). In this report we describe characterization monoclonal anti-idiotypic combining site 11C1 (Ab1), which recognizes shared determinant mouse (MMTV) and Hybridomas expressing internal image screened by an inhibition...
Cytotoxic T lymphocytes (CTL) are induced specifically against viral and tumor antigens presented by major histocompatibility complex class I molecules on the surface of infected or transformed cells. Intracellular synthesized processed associated with within cells before presentation cell surface. Because this special requirement for CTL induction, exogenous soluble do not, in general, induce specific responses. To overcome problem, various laboratories have resorted to use vaccinia virus...
In this study the tumor-specific immuneresponse induced by irradiated tumor cells (L1210/GZL) and anti-idiotype antibodies was analyzed. The (Ab2) were made against paratope of a monoclonal antitumor antibody (11C1) that recognizes tumor-associated antigen which cross-reacts with mouse mammary virus-encoded envelope glycoprotein 52. Two Ab2, 2F10 3A4, idiotypes expressed 11C1 2B2. Cytotoxic T cells, generated immunization lyse 3A4 hybridoma cells. Furthermore, Ab2 induces cytotoxic...
We have previously generated and used anti-Id mAb (Ab2) to induce protective immunity against the L1210 DBA/2 tumor for immunotherapy of established tumors. Among various that were typed serologically as internal image Ab2 mouse mammary virus tumor-associated Ag gp52, only one induced was effective in immunotherapy. In this study we compared structural, idiotypic, network properties nonprotective antiidiotypic antibodies. The DNA sequence variable regions six determined. VH four Ab2,...
In a previous report, we have demonstrated the induction of tumor-specific immunity by monoclonal anti-idiotype antibodies generated against anti-tumor antibody, 11C1, that also cross-reacts with mouse mammary tumor virus envelope glycoprotein gp52. Also, showed whereas one 2F10, could induce protective immunity, another 3A4, induced nonprotective immunity. Here existence T helper cells which recognize anti-idiotypes exert differential controls on growth. The qualitative nature idiotype...
Objectives: Investigating the role of nerve growth factor (NGF) and its receptors (NGF‐R) in inflammatory diseases is an active field research. Inflammatory joint are commonest cause human morbidity but very little known about effect NGF on synovial tissue biology. Here we have studied NGF/NGF‐R their functional significance cultured fibroblast‐like cells (FLS) collected from five healthy subjects.Methods: expression was determined basal condition after stimulation with tumour necrosis...
Herein we have analyzed the expression of idiotopes associated with a monoclonal anti-tumor-associated antigen (TAA) antibody in DBA/2 mice which progressively growing tumors or resist tumor growth. A panel eight antiidiotypic antibodies raised against reacts mouse mammary virus cross-reactive qp52 envelope protein L1210/GZL lymphoma was used to measure sera from different treatment groups. Significant correlations between certain and growth establishment anti-tumor immunity are seen. 1)...
As reported previously, 3A4 is a paratope-specific anti-idiotypic mAb and induces cellular humoral anti-tumor-associated Ag (TAA) responses. In this study, the specificity, MHC restriction processing requirement of Th cell lines clones were determined that recognize an idiotypic determinant (Id) on 3A4. The anti-Id-3A4 part tumor-associated network which involved in regulation immunity against DBA/2 L1210/GZL tumor. These 3A4-Id-specific T are phenotypically cells Id context class II...
The mouse monoclonal antibody MEM136 (mAb1) is directed against an epitope on human melanoma-associated proteoglycan antigen (MPG). This also present various normal and subhuman tissues. A murine anti-idiotope (anti-Id) (mAb2), designated I-Mel-2, was generated used as a surrogate for the MPG molecule. I-Mel-2 tested in cynomolgus monkeys (Macaca fascicularis) its ability to induce anti-MPG humoral responses. All immunized with Ab2 developed specific anti-anti-idiotype (Ab3) responses that...
This study investigated the gene expression of TGFβ isoforms and their receptors in chick retina, retinal pigment epithelium (RPE), choroid effects short-term imposed optical defocus.
6529 Background: Curative-intent therapy for patients with newly diagnosed AML centers on the use of cytarabine as part an intensive multi-drug induction regimen. Treatment-related toxicity may lead to stopping treatment, despite considerable risk inadequately treated AML. We compared rates protocol discontinuation due adverse events (AEs) in either two standard regimens (7+3 or high dose + idarubicin, IA) S1203 study (Garcia-Manero; Leukemia 2023). Methods: was a randomized <60 years old...
Preinjection of a low dose cyclophosphamide (Cy) (500 microgram/mouse) either delayed or inhibited tumor appearance following the inoculation transplantable 3-methylcholanthrene-induced fibrosarcomas in inbred male C3H/HeJ mice. This Cy decreased spleen weight by 13% and total cell count 23%. However, same could potentiate footpad swelling reaction (FPSR) measured against Staphylococcus aureus antigen. Splenic lymphocytes from Cy-treated animals showed increased blastogenic response...
Calcitriol is well known for its therapeutic efficacy in psoriasis, but mechanism of action still unclear. In this study, we tried to elucidate the precise calcitriol psoriasis.Proliferation and apoptosis studies were done determine effect on normal human epidermal keratinocytes (NHEKs) T lymphocytes. To relevant chemokines proteins real-time polymerase chain reaction modified reconstructed epidermis (RHE) an vitro model psoriasis. All experiments triplicate. Results expressed as mean ±...
This cohort study of data from 39 patients assesses whether the presence a dominant T-cell receptor clone in peripheral blood is associated with time to systemic treatment stage IB mycosis fungoides.
The effects of neonatal antigen exposure on the adult B cell repertoire have been examined by characterizing influenza hemagglutinin (HA)-specific response BALB/c mice given soon after birth. Ligand during early life exerts a profound and lasting effect upon repertoire, characterized expansion preservation particular antigen-reactive clones apparent loss others. precise subset clonotypes selectively preserved depends age at which is first encountered; predictable knowledge emerging primary...