A5103077031- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Viral Infections and Immunology Research
- Ecology and Conservation Studies
- Fire effects on ecosystems
- Vector-Borne Animal Diseases
- Animal Virus Infections Studies
- Respiratory viral infections research
- Viral Infections and Vectors
- Microbial Community Ecology and Physiology
- Polar Research and Ecology
- Cryospheric studies and observations
- Geology and Paleoclimatology Research
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Isotope Analysis in Ecology
Korea Research Institute of Standards and Science
2020-2023
Theragen
2023
Chungnam National University
2020-2022
Korea Research Institute of Chemical Technology
2020-2022
The World Health Organization (WHO) has declared the coronavirus disease 2019 (COVID-19) as an international health emergency. Current diagnostic tests are based on reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method, which is gold standard test that involves amplification of viral RNA. However, RT-qPCR assay limitations in terms sensitivity and quantification. In this study, we tested both qPCR droplet digital PCR (ddPCR) to detect low amounts cycle threshold (CT)...
Abstract Rift valley fever (RVF) is an important zoonotic disease caused by the virus (RVFV) which can affect ruminants and humans. In this study, a comparison was done of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) transcription-droplet digital PCR (RT-ddPCR) assays with synthesized RVFV RNA, cultured viral mock clinical RNA samples. The genomic segments (L, M, S) three strains (BIME01, Kenya56, ZH548) were used as templates for in vitro transcription (IVT). Both...
Abstract The World Health Organization (WHO) has declared the Coronavirus disease 2019 (COVID-19) as an international health emergency. Current diagnostic tests are based on reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method, gold standard test that involves amplification of viral RNA. However, RT-qPCR assay limitations in terms sensitivity and quantification. In this study, we tested both qPCR droplet digital PCR (ddPCR) to detect low amounts cycle threshold (CT)...
Rapid and accurate sequencing covering the entire genome is essential to identify genetic variations of viral pathogens. However, due low titers in clinical samples, certain amplification steps are required for sequencing. At present, there no universal primers available alphacoronaviruses that, since these viruses have diverse strains, new specific target strain must be continuously developed Thus, this study, we aimed develop a primer set valid all human applicable samples containing trace...
As SARS-CoV-2 variants of concern emerged, the genome sequencing strains became more important. In this study, was sequenced using amplicon-based with MinION. The primer panel used in study consisted only 11 panels and size amplicons approximately 3 kb. Full sequences were obtained a hundred copies genome, 92.33% 75.39% 10 genome. few differences nucleotide originated from mutations laboratory cultures and/or mixed sequences. quantification genomic RNA done RT-ddPCR methods, level LoD...
The human rhinovirus (RV) is a positive-stranded RNA virus that causes respiratory tract diseases affecting both the upper and lower halves of system. RV enhances its replication by concentrating synthesis within modified host membrane in an intracellular compartment. infections often occur alongside caused other viruses, may remain asymptomatic for extended periods. Alongside qualitative detection, it essential to accurately quantify from clinical samples explore relationships between viral...
Snow covers a large surface area of the Earth and provides for exchange biological chemical components. However, microbial composition components snow are poorly understood. We assessed bacterial fungal diversity characteristics in freshly deposited snowfall samples collected from sub-urban site Daejeon, Republic Korea. analyzed using DNA amplification followed by Illumina MiSeq Sequencing microbiome, ion chromatography cations (Na+, Ca2+, Mg2+, NH4+) anions (SO42−, NO3−, Cl−), water-soluble...
송국리형 주거지의 이용 및 폐기과정은 청동기시대의 흥미로운 연구 과제 중 하나이며, 특히 내부 흑색토층의 존재는 많은 관심을 끌어왔다. 송국리 유적 제24차와 25차 발굴조사에서 흑색토층 이 확인된 주거지(98호, 100호, 107호)를 대상으로 환경고고학적 연구(토양 미세형태분석 규소체 분석)를 수행하였다. 토양 결과, 흑색토층은 탄화물이 집적된 층으로 보이며 탄화 가 비교적 가까운 곳에서 일어났을 가능성을 시사한다. 아마도 지붕이나 벽체를 비롯한 상부 구조물 탄화되어 층일 가능성이 높다. 한편 100호 규소체분석 중간 퇴적토에서 벼와 조의 규소체가 다량으로 확인되었다. 그리고 사초과 식물의 규소체와 해면동물 골편이 출토된 것으로 보아 저지대(예, 수전) 토양을 벽체 등의 건축재로 사용했던 보인다. 또 한, 흑색토층이 보이는 주거지와 수혈유구는 연접한 경우가 많아, 화재에 의한 주거지 와 인근 수혈 내부로 흘러 들어간 생각된다. 이를 종합하여 보면 일부 주거지는 사용이...