A5030320590- Electrospun Nanofibers in Biomedical Applications
- Cellular Mechanics and Interactions
- Tendon Structure and Treatment
- Silk-based biomaterials and applications
- 3D Printing in Biomedical Research
- Force Microscopy Techniques and Applications
- Nerve injury and regeneration
- Optical Coherence Tomography Applications
- Elasticity and Material Modeling
- Bone Tissue Engineering Materials
- Photoacoustic and Ultrasonic Imaging
- Cell Adhesion Molecules Research
- Advanced Fluorescence Microscopy Techniques
- Orthopaedic implants and arthroplasty
- Urologic and reproductive health conditions
- Tissue Engineering and Regenerative Medicine
- Polymer Surface Interaction Studies
- Electrohydrodynamics and Fluid Dynamics
- Graphene and Nanomaterials Applications
- Testicular diseases and treatments
- Polymer Nanocomposite Synthesis and Irradiation
- Advanced Surface Polishing Techniques
- Urinary and Genital Oncology Studies
- Soft tissue tumor case studies
- Retinal Development and Disorders
Institute of Polymers, Composites and Biomaterials
2014-2021
National Research Council
2014-2021
Vrije Universiteit Amsterdam
2017-2020
Functional polymers currently represent a basic component of large range biological and biomedical applications including molecular release, tissue engineering, bio-sensing medical imaging. Advancements in these fields are driven by the use wide set biodegradable with controlled physical bio-interactive properties. In this context, microscopy techniques such as Atomic Force Microscopy (AFM) emerging fundamental tools to deeply investigate morphology structural properties at micro...
Electrospun polymeric fibers are currently used as 3D models for in vitro applications biomedical areas, i.e., tissue engineering, cell and drug delivery. The high customization of the electrospinning process offers numerous opportunities to manipulate control surface area, fiber diameter, density evaluate response cells under different morphological and/or biochemical stimuli. aim this study was investigate-via atomic force microscopy (AFM)-the chemical changes bi-component electrospun...
The investigation of the mechanical properties embryos is expected to provide valuable information on phenomenology morphogenesis. It thus believed that, by mapping viscoelastic features an embryo at different stages growth, it may be possible shed light role mechanics in embryonic development. To contribute this field, we present a new instrument that can determine spatiotemporal distributions over wide area and with unprecedented accuracy. method relies combining ferrule-top...
The discovery of new drugs to treat pathological cells in the case aggressive liver primary cancer is imposing identification high-throughput screening systems predict vivo response therapeutic molecules, order reduce current use animals and drug testing costs. Recently, micro/nanostructured scaffolds have been adopted reproduce hepatic microenvironment due their higher similarity biological niche with respect traditional two-dimensional culture plate, so providing novel vitro models for...
Surface topography and chemistry both play a crucial role on influencing cell response in 3D porous scaffolds terms of osteogenesis. Inorganic materials with peculiar morphology chemical functionalities may be proficiently used to improve scaffold properties—in the bulk along pore surface—promoting vitro vivo osseous tissue in-growth.
Mechanical forces are increasingly recognized as major regulators of several physiological processes at both the molecular and cellular level; therefore, a deep understanding sensing these their conversion into electrical signals essential for studying mechanosensitive properties soft biological tissues. To contribute to this field, we present dual-purpose device able mechanically stimulate retinal tissue record spiking activity ganglion cells (RGCs). This new instrument relies on combining...
Embryos are growing organisms with highly heterogeneous properties in space and time. Understanding the mechanical is a crucial prerequisite for investigation of morphogenesis. During last 10 years, new techniques have been developed to evaluate biological tissues vivo. To address this need, we employed instrument that, via combination micro-indentation Optical Coherence Tomography (OCT), allows us determine both, spatial distribution chick embryos, structural changes real-time. We report...
Recent studies have suggested that microenvironmental stimuli play a significant role in regulating cellular proliferation and migration, as well modulating self-renewal differentiation processes of mammary cells with stem cell (SCs) properties. advances micro/nanotechnology biomaterial synthesis/engineering currently enable the fabrication innovative tissue culture platforms suitable for maintenance SCs vitro. Here, we report design an open microfluidic device (OMD) integrating removable...
Abstract In the last decade, different technological approaches have been proposed for fabrication of micro‐gels as cell carriers to investigate in vitro response. Among them, electro fluid dynamic atomization (EFDAs) is emerging a highly versatile process that allows atomizing polymer solutions at micrometric size scale by application high voltage electric field. Here, we propose revisit configuration optimize sodium alginate with physical and mechanical properties validate their use...
We investigate and validate a novel method to fabricate ultrathin optical probes for common-path coherence tomography (CP-OCT). The are obtained using 65 μm barium titanate microsphere inserted into an inward concave cone chemically etched at the end of single-mode fiber. demonstrate that high refractive index (n=1.95) microspheres allows one maintain sensitivity even while imaging in liquids, reaching 83 dB. Due its low cost, flexibility, ease use, probe holds promise development new...
Bicomponent electrospun fibers (BEFs) have been designed as building blocks of various biomedical devices, i.e., scaffolds, conduits, grafts, for the repair/regeneration in vitro and vivo tissues. With respect to monocomponent fibers, BEF may exert diversified patterns signals—morphological, chemical or physical—confined at single fiber level, conferring them a unique ability self-adapting local microenvironment influencing cell/fiber interaction. Herein, an investigation basic relationships...
Summary statement We introduce an experimental technique that combines micro-indentation and optical coherence tomography to map the viscoelastic properties of embryonic tissue investigate correlations between local mechanical features morphology. Abstract The investigation embryos is expected provide valuable information on phenomenology morphogenesis. It thus believed that, by mapping embryo at different stages growth, it may be possible shed light role mechanics in development. To...
The majority of tissue related diseases are known to alter structure. During the last 10 years, increasing efforts have been put into development new techniques that could provide in vivo information on morphology. Optical coherence tomography (OCT) is structural situ. However, there also a strong demand evaluate mechanical properties biological tissues vivo. To address this need, we combined microindentation with non-invasive OCT imaging determine spatiotemporal distributions and...
Abstract Embryos are growing organisms with highly heterogeneous properties in space and time. Understanding the mechanical is a crucial prerequisite for investigation of morphogenesis. During last ten years, new techniques have been developed to evaluate biological tissues vivo . To address this need, we employed instrument that, via combination micro-indentation Optical Coherence Tomography (OCT), allows us determine both, spatial distribution chick embryos structural changes real-time...
Abstract We present a new optomechanical probe for mechanical testing of soft matter. The consists micromachined cantilever equipped with an indenting sphere, and array 16 single‐mode optical fibres, which are connected to coherence tomography (OCT) system that allows subsurface analysis the sample during indentation stroke. To test our device its capability, we performed on PDMS‐based phantom. Our findings demonstrate Common Path (CP)‐OCT via lensed fibres can be successfully combined...