Yarrowia lipolytica has been widely engineered as a eukaryotic cell factory to produce various important compounds. However, the difficulty of gene editing and lack efficient neutral sites make rewiring Y. metabolism challenging. Herein, Cas9 system was established redesign homologous recombination system, which caused more than 56-fold increase in HR efficiency. The fusion expression hBrex27 sequence C-terminus recruited Rad51 protein, decreased NHEJ, achieving 85% single-gene positive...

Cellulose utilization has been seriously hindered by the formation of humins, whereas humins structure remains challenging since unconverted cellulose and exist as solid mixture. In this work, we developed...

Abstract The recycling and utilization of waste cotton fabrics is great significance to implement green pollution reduction carbon reduction. In order realize the high value-added cellulose (WCC) by introducing new dots (CDs), WCC/CDs composite hydrogel with good antibacterial effect were prepared for application in field dressings. formed doping different contents (CDs) WCC as raw material epichlorohydrin (EPI) crosslinking agent. structure was characterized X-ray diffraction (XRD) infrared...

This study addresses the challenges of magnetic circuit coupling and control complexity in active radial bearings (ARMBs) by systematically investigating electromagnetic performance four pole configurations (NNSS, NSNS, NNNN, SSSS). Initially, equivalent modeling finite element analysis (FEA) were employed to analyze phenomena their effects on flux density distribution for each configuration. Subsequently, air gap force quantified under rotor eccentricity caused unbalanced disturbances,...

A chirp signal is a large-bandwidth which widely used in engineering. In many applications, it necessary to decompose mixed into its components. However, the traditional Fourier transform method cannot process when components intersect joint time-frequency domain. Combining advantages of morphological component analysis (MCA) with multicomponent processing and fractional (FrFT) processing, this letter proposes MCA-FrFT signal. First, cost function defined using FrFT optimized by split...

Intelligent control of space manipulator with flexible-link and flexible-joint is discussed based on the singular perturbation method. Owing to combined effects link joint flexibilities, dynamic model this kind becomes more complex leads a series unsolved system. To simplify design system, method used obtain two-time-scale simpler subsystem composite designed realize precise trajectory tracking vibration suppression simultaneously for flexible-link/flexible-joint payload. In slow subsystem,...

The water entry behaviors of projectiles with different cone-headed angles were studied numerically, experimentally and theoretically, mainly focusing on the hydrodynamic impact in initial stage. Based MMALE algorithm, it was proposed a formula deceleration, which relied velocity angle. Meanwhile, order to verify validity simulation model, experiments using accelerometer high-speed camera carried out, their results good agreement results. Also, theoretical calculation cavity diameter...

Unmanned Underwater Vehicle (UUV) is being widely developed and used in many areas. In order to meet the low resistance noise requirements of a kind UUV for long voyage, outline curve parameters different sections are figured out by using method superposition point-source uniform-flow, constant-strength-line-congruence empirical formula, thus total hydrodynamic obtained. addition, pressure distribution head analyzed, maximum motion velocity that cannot induce cavitation These results provide...

We theoretically investigate the gap function, superfluid density and transition temperature of superconductivity (SC) on semi-periodic Penrose lattice, where an attractive Hubbard model is adopted as example. Firstly, we clarify that states are all positively correlate to extended degree single particle around Fermi energy. Secondly, identify paramagnetic component does not decay zero in thermodynamic limit, which completely different from periodic system. The difference between diamagnetic...

As marine resource exploitation continues to advance, and the strategic significance of environments becomes increasingly prominent, demand for underwater vehicles in field science research is becoming progressively clear. The thrust vector control system, as a pivotal component vehicles, assumes crucial role governing movements these vehicles. In order enhance performance device based on DSP28335 platform was developed using dual closed-loop algorithm. Its feasibility validated through...

A new automatic fixed-point landing algorithm for UAV using the instantaneous speed obtained by image sensors and computer vision method is proposed. In proposed scheme, once specified land pad captured, will switch from auto-seeking mode to mode. mode, feature point of prospective zone extracted then being tracked. The noise in motion parameter introduced mismatching reduced fast iterative least square algorithm, accurate obtained. simulation results show that efficiently improve accuracy...

The increase of small targets on the sea surface poses a great threat to public security. Sea has extremely complex scattering characteristics and clutter is mixed with radar target echoes, which seriously affects detection performance for surface. Therefore, method based spikes identification matrix completion proposed in this paper. By analyzing spikes, echoes are distinguished eliminated from original signals. Then, unqualified data filled up by method, effectively improves...

Abstract The water-entry behaviours of projectiles with hemisphere-head were studied experimentally and theoretically, focusing on projectile dynamics drag coefficient. Based equivalent cone theory proposed by Baldwin, the equation coefficient was developed to describe resistance hemispherical head from time when contacts water is submerged. A set experimental apparatus up record process high-speed photography, variation during obtained experimentally. Compared acceleration calculated...

Abstract When the projectile enters into water at high velocity, impact force will be huge, therefore, it is necessary to protect projectile. In this paper, polyurethane foam selected study its protective properties for water-entry The test was designed compare numerical simulation results, in order verify feasibility and validity of model. particular, method used motion response characteristics unprotected protected results demonstrated that has good comparing effect different density by...

Abstract The structure will suffer a huge overload, when it enters into water. buffer head cap can effectively reduce the and material in is key to its load reduction performance. In order study buffering ability of aluminum foam, an effective numerical simulation method established this paper. observe motion energy absorption process foam. It found that foam has strong capacity reducing by comparing with without under same conditions. deformation, protect projectile from buckling deformation.

In the high-speed water impact experiment, we need to store and recover acceleration data generated during impact. response this demand, adopted a high anti-noise IEPE sensor, matched with corresponding signal conditioning module, used 24-bit high-precision A/D conversion expansion board develop an measurement device system plan. This scheme completes layout wiring of entire collection device. Aiming at power supply problem under overload, designed regulator IC conducted overload analysis....

Abstract In order to explore the influence of bevel angle projectile on vertical velocity attenuation characteristics and cavitation after it enters water vertically, this paper uses numerical simulation analyze angles 45°, 30°, 25° 20°. At same time, high-speed camera is used study penetration with a 45° verify model. The research results show that decays exponentially, trajectory truncated bent entering so an asymmetric open occurs. will affect degree curvature, shape cavity. larger angle,...