A5036728629- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Poxvirus research and outbreaks
- COVID-19 epidemiological studies
- Biosensors and Analytical Detection
- Microbial Community Ecology and Physiology
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Respiratory viral infections research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Bacillus and Francisella bacterial research
- Microfluidic and Capillary Electrophoresis Applications
Swiss Federal Institute of Aquatic Science and Technology
2023-2024
ETH Zurich
2023
Massachusetts Institute of Technology
2023
École Polytechnique Fédérale de Lausanne
2019
Monkeypox virus (MPXV) is a zoonotic pathogen that has recently caused outbreaks in non-endemic areas. Wastewater-based surveillance (WBS) offers substantial potential for monitoring MPVX clades, and it can inform population-level disease dynamics. Here, we report four-plex digital PCR assay to detect quantify different clades subclades of MPXV. The demonstrated specificity distinguishing quantifying MPXV by clade, which advantageous application both clinical wastewater settings.
Measles outbreaks remain a significant public health challenge despite high vaccination coverage in many regions. Wastewater-based surveillance (WBS) offers non-invasive and community-level approach to monitoring the circulation of pathogens, including measles virus. Here, we retrospectively applied duplex digital PCR assay distinguish between wild-type vaccine strains virus wastewater samples available from an existing national WBS program. Samples originated treatment plant serving...
As more data on virus concentrations in influent water from wastewater treatment plants (WWTPs) becomes available, establishing best practices for measurements, monitoring, and statistical modelling can improve the understanding of concentration distributions wastewater. To support this, we assessed temporal variability norovirus, adenovirus, enterovirus, rotavirus across multiple WWTPs Switzerland, USA, Japan. Our findings demonstrate that lognormal distribution accurately predicts...
Waterborne human viruses can persist in the environment, causing a risk to health over long periods of time. In this work, we demonstrate that both freshwater and seawater environments, indigenous bacteria protists graze on waterborne thereby reduce their persistence. We furthermore efficiency grazing process depends temperature, virus type, protist species. These findings may facilitate design biological methods for disinfection water wastewater.
ABSTRACT Wastewater-based surveillance systems can track trends in multiple pathogens simultaneously by leveraging efficient, streamlined laboratory processing. In Switzerland, wastewater is conducted for fourteen locations representing 2.3 million people, or 26% of the national population, with simultaneous four respiratory pathogens. Trends diseases are tracked using a novel, six-plex digital PCR assay targeting Influenza A, B, Respiratory Syncytial Virus, and SARS-CoV-2 N1 N2 genes, as...
Abstract Quantitative PCR (qPCR) and digital (dPCR) are applied for quantifying molecular targets in disease diagnostics, pathogen detection ecological monitoring. Uptake of dPCR is increasing due to its higher quantification accuracy relative qPCR which stems from independence standard curves increased resistance inhibitors. Throughput can be through multiplexing, allows simultaneous multiple targets. However, multiplexing with faces unique challenges qPCR. Here we describe the three-phase...
Abstract The COVID-19 pandemic has accelerated the development and adoption of wastewater-based epidemiology. Wastewater samples can provide genomic information for detecting assessing spread SARS-CoV-2 variants in communities estimating important epidemiological parameters such as growth advantage variant. However, despite demonstrated successes, data derived from wastewater suffers potential biases. Of particular concern are differential shedding profiles that different exhibit, because...
Abstract The COVID-19 pandemic has accelerated the development and adoption of wastewater-based epidemiology. Wastewater samples can provide genomic information for detecting assessing spread SARS-CoV-2 variants in communities estimating important epidemiological parameters such as growth advantage variant. However, despite demonstrated successes, data derived from wastewater suffers potential biases. Of particular concern are differential shedding profiles that different exhibit, because...