A5072972493- Biosensors and Analytical Detection
- Rock Mechanics and Modeling
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- SARS-CoV-2 detection and testing
- Landslides and related hazards
- Stability and Controllability of Differential Equations
- Tunneling and Rock Mechanics
- Toxic Organic Pollutants Impact
- Groundwater flow and contamination studies
- Soil and Unsaturated Flow
- Advanced Mathematical Modeling in Engineering
- Nanoparticles: synthesis and applications
- Advanced Nanomaterials in Catalysis
- Advanced Numerical Methods in Computational Mathematics
- Advanced Cellulose Research Studies
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- Urban Green Space and Health
- Dam Engineering and Safety
- Power Systems and Renewable Energy
- Nanocomposite Films for Food Packaging
- Protein Hydrolysis and Bioactive Peptides
- Ubiquitin and proteasome pathways
Virginia Tech
2019-2024
Swiss Federal Institute of Aquatic Science and Technology
2024
Seoul National University
2016-2019
Cornell University
2013
Sunchon National University
2006
University of Southern California
2002-2005
Kumamoto University
2000
Chosun University
1997-1998
Kyungpook National University
1995
Tokushima University
1991
ConspectusAntimicrobial resistance (AMR) is one of the greatest threats faced by humankind. The development in clinical and hospital settings has been well documented ever since initial discovery penicillin subsequent introduction sulfonamides as antibiotics. In contrast, environmental (i.e., community-acquired) dimensions dissemination have only more recently delineated. global spread antibiotic resistant bacteria (ARB) genes (ARGs) between air, water, soil, food now documented, while...
Bacterial cellulose nanocrystals (BCNCs) are tunable and biocompatible nanomaterials that can be easily bioconjugated used for biosensing applications. We report the application of concanavalin A (con A) lectin-modified BCNCs + BCNCs) bacterial isolation label-free surface-enhanced Raman spectroscopy (SERS) detection species using Au nanoparticles (AuNPs). The aggregated AuNP bacteria BCNC) conjugates generated SERS hot spots enabled strain Escherichia coli 8739 at 103 CFU/mL level....
We report label-free detection of 86-base single-stranded DNA (ssDNA) gene segments by surface-enhanced Raman spectroscopy (SERS). The use a slippery liquid infused porous (SLIP) membrane induced aggregation 43 nm gold nanoparticles and ssDNA upon pin-free droplet evaporation. combined SLIPSERS approach generates significant numbers SERS hot-spots enabled at the 100 nM level mecA intI1 segments—two genes interest in context antibiotic resistance. Tree-based multiclass support vector machine...
Label-free surface-enhanced Raman spectroscopy (SERS) has been proposed as a promising bacterial detection technique. However, the quality of collected spectra can be affected by time between sample acquisition and SERS measurement. This study evaluated how storage stress stimuli influence label-free Pseudomonas syringae samples stored in phosphate buffered saline. The results indicate that when faced with nutrient limitations changes osmatic pressure, at room temperature (25 °C) exhibit...
Ultrasensitive surface-enhanced Raman spectroscopy (SERS) still faces difficulties in quantitative analysis because of its susceptibility to local optical field variations at plasmonic hotspots metallo-dielectric nanostructures. Current SERS calibration approaches using tags have inherent limitations due spatial occupation competition with analyte molecules, spectral interference peaks, and photodegradation. Herein, we report that plasmon-enhanced electronic scattering (ERS) signals from...
Bacterial metabolites are intermediate products of bacterial metabolism and their production reflects metabolic activity. Herein, we report the use surface-enhanced Raman spectroscopy (SERS) for detection both volatile nonvolatile application this approach growth quantification diagnosis viral infection. The time-dependent SERS signal metabolite dimethyl disulfide in headspace above bacteria growing on an agar plate was detected quantified. In addition, signals arising from reflected...
The synthesis of superparamagnetic iron oxide nanoparticles (FeOx-NPs) has rapidly developed over the past decade due to their wide-ranging applications in research and technology. However, at present, there exists very limited knowledge about environmental impacts various input materials energy required for different FeOx-NP approaches. In this study, we used cradle-to-gate life cycle assessment (LCA) analyze compare FeOx-NPs produced via seven common routes. Four functional units (i.e.,...
This study aids in the identification of environmentally friendly and sustainable applications gold nanoparticles.
Influenza A virus poses significant public health challenges, causing seasonal outbreaks and pandemics. Its rapid evolution motivates continuous monitoring of circulating influenza genomes to inform vaccine antiviral development. Wastewater-based surveillance offers an unbiased, cost-effective approach for genomic surveillance. We developed a novel tiling amplicon primer panel that covers diversity virus, targeting segments the surface proteins HA, NA, M subtypes H1N1 H3N2. Using this panel,...
Cellulose nanomaterials are low cost, biocompatible and readily combined with other materials to produce nanocomposites a range of applications. have many advantageous properties, including high mechanical thermal stability, specific surface area, biodegradability. The highly flexible cellulose structure can be exploited transform bulk into isolated nanostructured fibers that retain the original thermal, mechanical, optical properties material. In this review, we highlight recent advances in...
Wastewater-based surveillance (WBS) has gained attention as a strategy to monitor and provide an early warning for disease outbreaks. Here, we applied isothermal gene amplification technique, reverse-transcription loop-mediated (RT-LAMP), coupled with nanopore sequencing (LAMPore) means detect SARS-CoV-2. Specifically, combined barcoding using both RT-LAMP primer the rapid kit achieve highly multiplexed detection of SARS-CoV-2 in wastewater. targeting N region was conducted on 96 reactions...
The proteolytic activities of the 20 S proteasome were found to change in their levels during development chick embryonic muscle.The peptide-cleaving against N-succinyl-Leu-Leu-Val-Tyr-7amido-4-methylcoumarin and N-benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-&naphthylamide gradually decreased with time development.On other hand, casein-degrading activity presence poly-L-lysine markedly increased from day 11 reached a maximal level by 17.These changes appeared be tissue-specific because little or...
Many outbreaks of emerging disease ( e.g. , avian influenza, COVID-19) are enhanced by the environmental dissemination viruses. Nanotechnology enabled tools have potential to detect viruses in complex matrices.
Surface-enhanced Raman spectroscopy (SERS) has great potential as an analytical technique for environmental analyses.
Bacterial cellulose nanocrystals (BCNCs) are biocompatible nanomaterials that can host guest nanoparticles to form hybrid nanocomposites with a wide range of applications.
Compatibility in a range of media is vitally important for surface-enhanced Raman scattering (SERS)-enabled pH detection. We report universal detection using top-down nanostructured gold SERS substrates and multivariate regression. with vertically stacked multiple nanogap hotspots functionalized the sensing molecule 4-mercaptopyridine (4-Mpy) exhibited high spatial uniformity. Standard ratiometric enabled development Boltzmann equation-based calibration curve phosphate-buffered saline. This...