A5010465475- Osteoarthritis Treatment and Mechanisms
- Silk-based biomaterials and applications
- Cancer, Hypoxia, and Metabolism
- Cardiac electrophysiology and arrhythmias
- Proteoglycans and glycosaminoglycans research
- Tendon Structure and Treatment
- Neuroscience and Neural Engineering
- Bone Metabolism and Diseases
- RNA Research and Splicing
- RNA modifications and cancer
- Protein Kinase Regulation and GTPase Signaling
- Cancer Cells and Metastasis
- Ion channel regulation and function
- Cell Adhesion Molecules Research
- TGF-β signaling in diseases
- Neurobiology and Insect Physiology Research
- Insect Pest Control Strategies
- Herbal Medicine Research Studies
- Connexins and lens biology
- Heat shock proteins research
- Glycosylation and Glycoproteins Research
- Fibroblast Growth Factor Research
- Head and Neck Anomalies
- Wnt/β-catenin signaling in development and cancer
- Cellular Mechanics and Interactions
Kyungpook National University
2012-2022
University of Iowa
1993
Cornell University
1985-1988
To study Na+-Ca2+ exchange, intracellular Na+ activity (aiNa), twitch tension, and transmembrane potential were simultaneously measured in canine cardiac Purkinje fibers driven at a constant rate (1 Hz) the absence presence of strophanthidin (5 X 10(-7) M) normal, low, high extracellular [Na+] ([Na+]o) or [Ca2+] ([Ca2+]o). Intracellular Ca2+ (aiCa) was also normal Tyrode solution. Reductions [Na+]o by 20, 40, 60% decreased ratio (aoNa) aiNa steady state but steeply increased tension. This...
Pre-mRNA processing factor (PRPF) 4B kinase belongs to the CDK-like family, and is involved in pre-mRNA splicing, signal transduction. In this study, we observed that PRPF overexpression decreased intracellular levels of reactive oxygen species, inhibited resveratrol-induced apoptosis by activating cell survival signaling proteins NFκB, ERK, c-MYC HCT116 human colon cancer cells. altered cellular morphology, rearranged actin cytoskeleton, regulating activity Rho family proteins. Moreover, it...
Abstract Disruption of the actin cytoskeleton in subconfluent mesenchymal cells induces chondrogenic differentiation via protein kinase C (PKC) α signaling. In this study, we investigated role p38 mitogen‐activated (MAP) that is induced by depolymerization cytoskeleton. Treatment derived from chick embryonic limb buds with cytochalasin D (CD) disrupted concomitant differentiation. The chondrogenesis was accompanied an increase MAP activity and inhibition SB203580 blocked chondrogenesis....
Abstract Cell shape alterations and accompanying cytoskeletal changes have diverse effects on cell function. We already shown that dedifferentiated chondrocytes a round morphology undergo redifferentiation when cultured chitosan membrane. In the present study, we investigate role of cytoskeleton in chondrocyte redifferentiation. Chondrocytes obtained from micromass culture chick limb bud mesenchymal cells were subcultured four times. Immunofluorescence analysis F‐actin showed cortical...
The effect of norepinephrine on the Na+-K+ pump was investigated by simultaneously measuring intracellular sodium ion activity (aiNa) and contractile force canine cardiac Purkinje fibers driven at 1.0 Hz in K+-free solution, high K+ presence tetrodotoxin. In Tyrode solution containing 5.4mM [K+]o, 10(-6) M decreased aiNa, whereas did not lower aiNa. 16.2 mM [K+]o aiNa from 8.8 +/- 0.9 to 6.5 0.5 (mean SD, n = 5). Exposure further 0.7 0.4 mM. This decrease prevented exposure 2.5 X...
The small GTPase protein Rac1 is involved in a wide range of biological processes including cell differentiation. Previously, was shown to promote chondrogenesis micromass cultures limb mesenchyme. However, the pathways mediating Rac1's role are not fully understood. This study aimed explore molecular mechanisms by which regulates chondrogenic Phosphorylation signal transducer and activator transcription 3 (STAT3) increased as proceeded chick wing bud Inhibition with NSC23766, janus kinase 2...
Conventional practices of synthesis, manufacturing, and processing have led to severe adverse consequences for living beings the environment.Although medications cannot be replaced, methods synthesizing, them can changed and/or replaced. This paper explains significance green chemistry in pharmaceutical industry. It emphasizes that we must replace conventional drug processing, manufacturing techniques with greener ones are cost-effective, sustainable, environment-friendly, profitable.This...
Transforming growth factor-β (TGF-β) plays crucial roles in controlling cell differentiation and maintaining tissue integrity. Previously we reported that TGF-β3 treatment decreased the mRNA expression of gap junction protein, connexin 43 as well number, which lead to inhibition chondrogenic condensation cultured chick leg bud mesenchymal cells. The present study demonstrates can induce cleavage ectodomain neuronal cadherin (N-cadherin) at initiation stage chondrogenesis reduce numbers,...
To investigate the effects of chitosan on redifferentiation dedifferentiated chondrocytes, we used chondrocytes obtained from a micromass culture system. Micromass cultures chick wing bud mesenchymal cells yielded differentiated but these during serial monolayer subculture. When were cultured membranes they regained phenotype chondrocytes. Expression protein kinase Cα (PKCα) increased chondrogenesis, decreased dedifferentiation, and again redifferentiation. Treatment with phorbol...
Drug repositioning has garnered attention as an alternative strategy to the discovery and development of novel anticancer drug candidates. In this study, we screened 321 FDA-approved drugs against nonirradiated irradiated MCF-7 cells, revealing that aripiprazole, a dopamine receptor D2 (D2R) partial agonist, enhances radiosensitivity cells. Unexpectedly, D2R-selective antagonist treatment significantly enhanced radiosensitizing effects aripiprazole prevented aripiprazole-induced 5' adenosine...
Pelvic and abdominal radiotherapy plays an important role in eradication of malignant cells; however, it also results slight intestinal injury. The apoptosis cells the epithelium is a primary pathological factor that initiates radiation-induced Auranofin, gold-containing triethylphosphine, was approved for treatment rheumatoid arthritis, its therapeutic application has been expanded to number other diseases, such as parasitic infections, neurodegenerative disorders, AIDS, bacterial...
We have investigated the involvement of pre-mRNA processing factor 4B (PRP4) kinase domain in mediating drug resistance. HCT116 cells were treated with curcumin, and apoptosis was assessed based on flow cytometry generation reactive oxygen species (ROS). Cells then transfected PRP4 or pre-mRNA-processing-splicing 8 (PRP8), resistance analyzed both
Recently Na + -selective microelectrodes (NaSM) have been used to measure quantitatively small changes in intracellular sodium ion activity [Formula: see text] and determine a precise time course of comparatively rapid change text]. In such studies, accurate measurement requires the following criteria: (i) NaSM should fast response (ii) an conventional voltage microelectrode same membrane potential. These criteria were evaluated by measuring when potential cardiac Purkinje fibers was...