A5063086787- Cardiac Structural Anomalies and Repair
- Cardiovascular Function and Risk Factors
- Cardiac Valve Diseases and Treatments
- Cardiomyopathy and Myosin Studies
- Sperm and Testicular Function
- Mechanical Circulatory Support Devices
- Respiratory Support and Mechanisms
- Elasticity and Material Modeling
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Reproductive Biology and Fertility
- Airway Management and Intubation Techniques
- Cardiac electrophysiology and arrhythmias
- Lipid Membrane Structure and Behavior
- Cardiac, Anesthesia and Surgical Outcomes
- Microfluidic and Bio-sensing Technologies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cardiac Arrest and Resuscitation
- Erythrocyte Function and Pathophysiology
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Epigenetics and DNA Methylation
- Analytical Chemistry and Sensors
- Cardiac Arrhythmias and Treatments
University of Münster
2006-2021
University Hospital Münster
2004-2020
Klinik und Poliklinik für Psychosomatik und Psychotherapie
2018
Institute of Reproductive Medicine
2013
Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde
1997-2003
Heinrich Heine University Düsseldorf
1998
Universität Ulm
1996
Fischer (Germany)
1994
Stuttgart Observatory
1994
Universitätsmedizin Greifswald
1993
Abstract Concepts for ventricular function tend to assume that the majority of myocardial cells are aligned with their long axes parallel epicardial surface. We aimed validate existence aggregates orientated axis intruding obliquely between and endocardial surfaces quantitate amount angulation. To compensate changing angle myocytes relative equatorial plane ventricles varying depths within walls, so‐called helical angle, we used pairs cylindrical knives different diameters punch semicircular...
Abstract Life‐long sperm production leads to the assumption that male fecundity remains unchanged throughout life. However, recently it was shown paternal age has profound consequences for fertility and offspring health. Paternal effects are caused by an accumulation of germ cell mutations over time, causing severe congenital diseases. Apart from these well‐described cases, molecular patterns ageing in cells their impact on DNA integrity have not been studied detail. In this study, we aimed...
There is lack of consensus concerning the three-dimensional arrangement myocytes within ventricular muscle masses. Bioengineers are seeking to model structure heart. Although success such models depends on accuracy anatomic evidence, most them have been based concepts that far from anatomical reality, which ignore many significant previous accounts anatomy presented over past 400 years. During 19th century, Pettigrew emphasized heart was built basis a modified blood vessel rather than in...
The macroscopical structure of the ventricular myocardium has been an unsolved problem since XVIth century, when Anatomy started as authentic science. Since then spatial organization myocardial fibres represented, Pettigrew s
One of the consequences oxygen metabolism is production reactive species (ROS) which in a situation imbalance with antioxidants can damage several biomolecules, compromise cell function and even lead to cellular death. The particularities sperm make it particularly vulnerable ROS attack compromising its functionality, mirrored terms fertility outcome making study origin ROS, as well alterations they cause very important. In present work, we used UVB irradiation, an easy experimental approach...
Can enzymatically dispersed testicular cells from adult men reassemble into seminiferous cord-like structures in vitro? Adult human somatic reassembled via dynamic interactions of Sertoli and peritubular cells. In vitro approaches using single cell suspensions testes to generate tubule initiate their functionality have as yet shown only limited success. Testes 15 gender dysphoria patients (mean ± standard deviation age 35 9.3 years) showing spermatogonial arrest became available for this...
Pairs of cylindrical knives were used to punch semicircular slices from the left basal, sub-basal, equatorial, and apical ventricular wall porcine hearts. The sections extended epicardium endocardium. Their shape compensated for depth-related changing orientation myocytes relative equatorial plane. analyzed by diffusion tensor magnetic resonance imaging. primary eigenvector was determined in each pixel calculate number angle intrusion long axis aggregated epicardial surface. Arrays axially...
Nucleotide polymorphisms in the VKORC1 gene can be linked to anticoagulant rodenticide resistance Norway rats (Rattus norvegicus Berkenhout). This provides a fitness advantage exposed actives, but may also cause costs. The vitamin K requirement and reproductive parameters of bromadiolone-resistant (Westphalian resistant strain; VKOR variant Tyr139Cys) bromadiolone-susceptible were compared.At deficiency, blood clotting times increased all homozygous males within 8 days 80% females 15 days....
Raman Microspectroscopy represents an innovative tool for the assessment of sperm biochemical features otherwise undetectable by routine semen analysis. Previously, it was shown that induced DNA damage can be detected in smeared this technique. This novel readout may value clinical settings especially if transferred to living cells. Yet, starting with sperms study carried-out using a variety conditions disclose nuclei under different hydration and UV exposure. Human were immobilized spectra...
Establishment and maintenance of the correct epigenetic code is essential for a plethora physiological pathways disturbed patterns can provoke severe consequences, e.g. tumour formation. In recent years, drugs altering epigenome tumours actively have been developed anti-cancer therapies. However, such could potentially also affect other systems in which intact are essential. Amongst those, male fertility one most prominent. Consequently, we addressed possible direct effects two drugs,...
Is Raman micro-spectroscopy a valid approach to assess the biochemical hallmarks of sperm regions (head, midpiece and tail) in four different species?SUMMARY ANSWER: Non-invasive provides spectral patterns enabling characterization three species, revealing however high similarities for each region among species.WHAT IS KNOWN ALREADY: has been described as an innovative method features having potential be used non-invasive selection tool.However, except nuclear DNA, identification assignment...
Marmosets are used as preclinical model in reproductive research. In contrast to other primates, they display short gestation times rendering this species valid for exploration of effects on fertility. However, their peculiar endocrine regulation differs from a those macaques and humans. We subjected male marmosets previously clinically tested hormonal regimens that known effectively suppress spermatogenesis. Beside control group, seven groups (each n=6) were investigated different periods...
Sperm DNA integrity has become one of the most discussed and promising biomarkers for assessment male fertility. However, an easy-to-apply method capable estimating fragmentation in live fraction spermatozoa remained elusive, preventing this parameter from being fully applied clinical settings.To validate a novel co-staining analysis membrane-intact spermatozoa.Normozoospermic semen samples were used to consisting acridine orange (AO) LIVE/DEAD™ Fixable Blue Dead Cell Stain (LD), against...
We used the technique of peeling myocardial aggregates, usually described as 'fibres', to determine spatial arrangement myocytes in left ventricular wall a healthy autopsied human heart.We digitised outer and inner boundaries, well pathways space, almost 3000 aggregates harvested from myocardium. During process gradual peeling, we sought identify uniformly possible. Despite this, interpolation was necessary complete pattern so construct unit vector field that represented preferred direction...
Myocardial contractile pathways which are not aligned strictly parallel to the heart's epicardial surface, give rise forces also act in ventricular dilating direction. We developed a method allows us assess any fibre orientation three-dimensional myocardial weave. Decollagenized hearts were prepared by peeling-off strands, following their main down near endocardium. In subepicardium strands followed course more or less epicardium, whereas from mid-wall on they tended dive progressively...