A5064276601- CRISPR and Genetic Engineering
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Insect behavior and control techniques
- Advanced Algebra and Geometry
- Electromagnetic Fields and Biological Effects
- Cerebral Palsy and Movement Disorders
- Traumatic Brain Injury Research
- Insect Resistance and Genetics
- COVID-19 epidemiological studies
- Survey Methodology and Nonresponse
- Homotopy and Cohomology in Algebraic Topology
- Insect and Pesticide Research
- Food Security and Health in Diverse Populations
- RNA regulation and disease
- Viral Infections and Vectors
- Cardiac Arrest and Resuscitation
- Statistical Methods and Bayesian Inference
- COVID-19 Clinical Research Studies
- Noise Effects and Management
- Air Quality and Health Impacts
University of California, San Diego
2020-2024
University of California, Berkeley
2023-2024
Czech Academy of Sciences, Institute of Biophysics
2023
CSIRO Health and Biosecurity
2023
National Health Research Institutes
2023
Commonwealth Scientific and Industrial Research Organisation
2023
University of York
2023
Universidad Cristiana Autónoma de Nicaragua
2023
Universidad Católica Santo Domingo
2023
Children's Hospital at Westmead
2020
There is increasing interest in estimating and drawing inferences about risk or prevalence ratios differences instead of odds the regression setting. Recent publications have shown how GENMOD procedure SAS (SAS Institute Inc., Cary, North Carolina) can be used to estimate these parameters non-population-based studies. In this paper, authors show model-adjusted risks, differences, ratio estimates obtained directly from logistic models complex sample survey setting yield population-based...
School closures have occurred globally during the COVID-19 pandemic. However, empiric data on transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among children and in educational settings are scarce. In Australia, most schools remained open first epidemic wave, albeit with reduced student physical attendance at peak. We examined SARS-CoV-2 staff early childhood education care (ECEC) Australian state New South Wales (NSW).Laboratory-confirmed paediatric (aged ≤18...
The development of an extensive toolkit for potential point-of-care diagnostics that is expeditiously adaptable to new emerging pathogens critical public health importance. Recently, a number novel CRISPR-based have been developed detect SARS-CoV-2. Herein, we outline the alternative CRISPR nucleic acid diagnostic utilizing Cas13d ribonuclease derived from
Full text Figures and data Peer review Side by side Abstract eLife assessment Introduction Results Discussion Methods Data availability References Article author information Metrics Each year, hundreds of millions people are infected with arboviruses such as dengue, yellow fever, chikungunya, Zika, which all primarily spread the notorious mosquito Aedes aegypti. Traditional control measures have proven insufficient, necessitating innovations. In response, here we generate a next-generation...
Each year, hundreds of millions people are infected with arboviruses such as dengue, yellow fever, chikungunya, and Zika, which all primarily spread by the notorious mosquito Aedes aegypti . Traditional control measures have proven insufficient, necessitating innovations. In response, here we generate a next-generation CRISPR-based precision-guided sterile insect technique (pgSIT) for Ae. that disrupts genes essential sex determination fertility, producing predominantly males can be deployed...
Escalating vector disease burdens pose significant global health risks, as such innovative tools for targeting mosquitoes are critical. CRISPR-Cas technologies have played a crucial role in developing powerful genome manipulation various eukaryotic organisms. Although considerable efforts focused on utilizing class II type CRISPR-Cas9 systems DNA targeting, these modalities unable to target RNA molecules, limiting their utility against viruses. Recently, the Cas13 family has emerged an...
Escalating vector disease burdens pose significant global health risks, so innovative tools for targeting mosquitoes are critical. We engineered an antiviral strategy termed REAPER (v
To investigate whether use of electric blankets, one the largest sources electromagnetic field exposure in home, is associated with risk female breast cancer, authors analyzed data from a population-based US case-control study. The 2,199 case patients were under age 55 years and had been newly diagnosed cancer between 1990 1992. 2,009 controls frequency-matched to cases by 5-year group geographic area. There was little or no ever having used mattress pads, heated water beds among women 45...
Abstract The recent discovery of the type III-E CRISPR-Cas effector class has reshaped our fundamental understanding evolution and classification. Type effectors are composed several Cas7-like domains a single Cas11-like domain naturally fused together to create polypeptide capable programmably targeting degrading RNA. Here we identify novel composition III-E-like Cas1-like domain, that can be engineered into an active chimeric RNA-targeting Cas presents new function Cas1 in RNA-targeting....
Abstract Since its first emergence from China in late 2019, the SARS-CoV-2 virus has spread globally despite unprecedented containment efforts, resulting a catastrophic worldwide pandemic. Successful identification and isolation of infected individuals can drastically curtail limit outbreaks. However, during early stages global transmission, point-of-care diagnostics were largely unavailable continue to remain difficult procure, greatly inhibiting public health efforts mitigate spread....
Abstract Each year, hundreds of millions people are infected with arboviruses such as dengue, yellow fever, chikungunya, and Zika, which all primarily spread by the notorious mosquito Aedes aegypti . Traditional control measures have proven insufficient, necessitating innovations. In response, here we generate a next generation CRISPR-based precision-guided sterile insect technique (pgSIT) for that disrupts genes essential sex determination fertility, producing predominantly males can be...
CasRx, a member of the RNA-targeting Cas13 family, is promising new addition CRISPR/Cas technologies in efficient gene transcript reduction with an attractive off-target profile at both cellular and organismal levels. It recently reported that CRISPR/CasRx system can be used to achieve ubiquitous tissue-specific Drosophila melanogaster. This paper details methods from recent work, consisting three parts: 1) vivo endogenous RNA targeting using two-component CasRx system; 2) exogenous...
Each year, hundreds of millions people are infected with arboviruses such as dengue, yellow fever, chikungunya, and Zika, which all primarily spread by the notorious mosquito
Each year, hundreds of millions people are infected with arboviruses such as dengue, yellow fever, chikungunya, and Zika, which all primarily spread by the notorious mosquito Aedes aegypti . Traditional control measures have proven insufficient, necessitating innovations. In response, here we generate a next generation CRISPR-based precision-guided sterile insect technique (pgSIT) for that disrupts genes essential sex determination fertility, producing predominantly males can be deployed at...
Each year, hundreds of millions people are infected with arboviruses such as dengue, yellow fever, chikungunya, and Zika, which all primarily spread by the notorious mosquito Aedes aegypti . Traditional control measures have proven insufficient, necessitating innovations. In response, here we generate a next generation CRISPR-based precision-guided sterile insect technique (pgSIT) for that disrupts genes essential sex determination fertility, producing predominantly males can be deployed at...
Consider a smooth projective curve and given embedding into space via sufficiently positive line bundle. We can form the secant variety of $k$-planes through curve. These are singular varieties, with each being along last. study invariants singularities for these varieties. In case an arbitrary curve, we compute intersection cohomology in terms then turn our attention to rational normal curves. this setting, prove that all varieties homology manifolds, meaning their satisfies Poincar\'e...
Type III-E CRISPR-Cas effectors, of which Cas7-11 is the first, are single proteins that cleave target RNAs without nonspecific collateral cleavage, opening new possibilities for RNA editing. Biochemical experiments combined with amide hydrogen-deuterium exchange (HDX-MS) provide a first glimpse conformational dynamics apo Cas7-11. HDX-MS revealed backbone comprised four Cas7 zinc-binding RRM folds well-folded but insertion sequences highly dynamic and fold upon binding crRNA. The crRNA...
Abstract Escalating vector disease burdens pose significant global health risks, so innovative tools for targeting mosquitoes are critical. We engineered an antiviral strategy termed REAPER (v R NA E xpression A ctivates P oisonous ffector ibonuclease) that leverages the programmable RNA-targeting capabilities of CRISPR Cas13 and its potent collateral activity. Akin to a stealthy Trojan Horse hiding in stealth awaiting presence enemy, remains concealed within mosquito until infectious blood...