A5017640109- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Banana Cultivation and Research
- Plant Virus Research Studies
- Plant Reproductive Biology
- Seed Germination and Physiology
- Composting and Vermicomposting Techniques
- Berry genetics and cultivation research
- Plant Genetic and Mutation Studies
- Plant and soil sciences
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
Fruit Research Institute
2022
Abstract The purpose of this work was to apply V and D cryo-plate methods for cryopreservation Vaccinium corymbosum ʻToroʼ, Fragaria × ananassa ʻCleryʼ, Amelanchier alnifolia (Nutt.) M. Roem. monitor the multiplication capacity shoots regenerated from cryopreserved explants. Shoot tips pre-cultured 1 d at 23°C in dark on medium containing 0.3 M sucrose were used as Loading performed a solution 2 glycerol 0.8 (30 min room temperature). In cryo-plate, dehydration carried out temperature (20 50...
Healthy plant material that is uniform and genetically pure essential for the successful raspberries production since it guarantees quality consistent yields. A 4-year study (2018–2021) focused on raspberry cultivars “Meeker” “Willamette”, which were propagated by tissue culture (MpP) root suckering (RsP) methods in three irrigation regimes (IR1-100% ETc, IR2-50% IR3-25% ETc). had longer primocanes, higher productivity, sweetness, soluble solids, sugar content IR1. Micropropagated plants...
This research investigated the influence of propagation methods and harvesting time on fruit quality two raspberry cultivars, ‘Meeker’ ‘Willamette’. The plants were propagated through tissue cultre root suckering, fruits picked during three harvest in 2020. study analyzed soluble solids content (SSC), total sugars (TS), titratable acidity (TA), phenolic anthocyanin (TPC TAC), as well antioxidant activity (AA). results showed that SSC TS increased progressed, while TA decreased. higher...
The objective of this study is to assess the suitability vitrification cryo-plate (V cryo-plate) and dehydration (D methods for long-term conservation eight autochthonous Prunus domestica L. genotypes originating from Balkan Peninsula region. In vitro shoot tips were briefly pre-cultured 1 day at 23 °C in dark on a medium containing 0.3 M sucrose then embedded calcium alginate gel within wells aluminum cryo-plates. V protocol, was carried out room temperature using following solutions:...
The success of slow-growth storage relies on the interaction multiple factors. However, despite having a well-designed, species-specific protocol, response to can exhibit notable differences among genotypes, even within same species. paper gives summary achievements and results in conservation several fruit species utilising cold technology achieved at Fruit Research Institute, Čačak. survival rates vitro cultures plums (‘Požegača’, ‘Sitnica’, ‘Crvena Ranka’, Cherry plum), cherry rootstocks...
Removing contaminants from plant material with minimal damage to cells using different chemical solutions represents very important and one of the most critical steps in establishing tissue culture vitro. Our study deals optimisation protocol for successful surface sterilization initial explants two raspberry cultivars, 'Meeker' 'Willamette' experimental years. The main objective during first year was examine protocols which included combinations disinfectants (mercuric chloride or...
For the purpose of removing viruses from infected plant material, cryotherapy is a novel application cryopreservation technique. The use various procedures and treatments necessitates examination cryo-treated material for presence targeted pathogen in order to gauge effectiveness therapy. In our study, we evaluated efficiency reverse transcription-polymerase chain reaction (RT-PCR) qPCR (quantitative PCR) methods detection plum pox virus (PPV) plums ‘Belošljiva’ ‘Crvena Ranka’. A qRCR assay...
We presented the results about preserving in vitro selected fruit genotypes by cold storage strategy and capacity for following shoot multiplication.The investigation included five genotypes: plum 'Po`ega~a', cherry rootstocks ('Gisela 5' 'Tabel Edabriz'), raspberry 'Meeker', blackberry '^a~anska Bestrna'.The best were achieved with shoots of cultivar 'Po`ega~a' which can be successfully stored at 5°C up to 10 months, without subculturing producing viable shoots.Furthermore, a significant...
Abstract Conservation of plant species, particularly those important for agriculture, has now reached a very high importance in order to ensure sustainable utilization biological resources by preventing further losses diversity. The most advanced method conservation is cryopreservation. Cryotherapy that relies on cryopreservation procedure, alone or combination with other techniques, can be used pathogen elimination from infected plants. Viral diseases pose significant threat the decreasing...