Abstract The purpose of this work was to apply V and D cryo-plate methods for cryopreservation Vaccinium corymbosum ʻToroʼ, Fragaria × ananassa ʻCleryʼ, Amelanchier alnifolia (Nutt.) M. Roem. monitor the multiplication capacity shoots regenerated from cryopreserved explants. Shoot tips pre-cultured 1 d at 23°C in dark on medium containing 0.3 M sucrose were used as Loading performed a solution 2 glycerol 0.8 (30 min room temperature). In cryo-plate, dehydration carried out temperature (20 50...

Murashige and Skoog medium (MS) modified Anderson's Rhododendron (mAN) were compared for in vitro shoot multiplication of three highbush blueberries 'Berkeley', 'Bluecrop' 'Goldtraube'. All media contained 0.5 mg l−1 zeatin applied eit

Two selected mutant lines, C26 and C82, with highly significant 1000-seed weight generated through irradiation treatment of Amaranthus cruentus L. 'Ficha', were evaluated compared to an original nontreated control reference variety Aztec. The phenotypic traits biochemical properties the amaranth seeds studied. Comparable values for crude protein content, albumins, globulins fractions overall coefficients nutritional quality found in samples. However, showed significantly lower concentrations...

Three types of callus tissues established from anther culture eleven doubled haploid (DH) lines wheat (Triticum aestivum L.) were evaluated for their ability in enhancing friable embryogenic (Type II) differentiation and genetic transformation. Differences between inocula highly significant (P<0.001), suggesting that the quality initial explant is profound importance encouraging proliferation Type II cultures. Other factors found to be crucial included weekly subculture on a maintenance...

Water-insoluble β-(1,3)-D-glucan (lichenan) was determined in 43 samples of various cereal (oats, barley, wheat, millet) and pseudocereal (buckwheat, amaranth) cultivars using a modified procedure with fungal α-amylase (Fermizyme P 300). The content water-insoluble β-glucan varied the dependence on species cultivars. highest registered covered oat (Cyril new breeding cultivar PS-100) ranging from 26.7 to 28.2 g/100 g dry matter (d.m.) followed by less traditional cereals such as millet...

Somatic embryogenesis was initiated from immature zygotic embryos of intraspecific cross ofAbies alba and interspecific combination x Abies nordmanniana. The most responsive explants for induction embryogenic calli were in precotyledonary stage development. Biochemically the compared lines uniform irrespective their morphology or potential. flow cytometric analysis single suggests that no changes ploidy levels occur during culture callus inAbies what confirms convenience this system...

Maturation and germination of somatic embryos hybrids A. alba × nordmanniana were followed by protein analysis single embryogenic -suspensor masses (ESM) storage accumulation during embryo development. Very important step was one week pre-cultivation ESM on medium with polyethylene glycol (PEG) abscisic acid (ABA). Low osmotic potential maturation addition ABA supported development embryo. Also partial drying following three weeks needed for its normal In spite morphologically fully...

The influence of initial medium pH on long-term axillary shoot proliferation was tested in Anderson's media supplemented with 0.5 mg.l -1 zeatin adjusted to 4.0, 4.5, 5.0, 5.5 and 6.0 before autoclaving, the aim increasing Vaccinium vitis-idaea L. cv.Koralle Red Pearl.Shoot using in-vitro-derived single-node segments markedly influenced by cultivar pH.Shoot significantly higher (6.92 shoots/explant) than cv.Red Pearl (5.61 at different culture pH.The study confirmed importance properly for...

The aim of this work was to evaluate shoot multiplication two blackberry (Rubus fruticosus L.) cultivars ?Black Satin? and ?Loch Ness? on culture media: Murashige &amp; Skoog (MS) its modification Van der Salm medium (MS VDS) which differ only in iron source (FeNaEDTA, FeEDDHA, respectively). Statistical analyses showed no significant difference between different sources for both tested cultivars. For it shown that double concentration chelates FeNaEDTA FeEDDHA media negatively affected...

Berry fruit species are, in many countries, considered biologically and economically valuable important of small fruits. The aim this work was to examine the influence either decreased or increased mesos concentrations (MgSO4, CaCl2, KH2PO4) on shoot multiplication five cultivars three (Amelanchier alnifolia var. cusickii, Rubus fruticosus 'Black Satin' 'Loch Ness', Vaccinium corymbosum 'Brigitta Blue' 'Toro'). Mesos nutrients were manipulated from half four times their base concentration....

Amaranth species represent a diverse group of plants. Many them are rich source secondary metabolites with many positive biological effects. Total phenolic, total flavonoid and rutin content, antioxidant activity against superoxide hydroxyl radicals, FRAP (Ferric-reducing ability plasma) assay DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging were determined in ethanol extracts dried leaves the new Slovak amaranth varieties ‘Pribina’ ‘Zobor’. The amount phenolic substances (‘Pribina’...

After “floral-dip” transformation of Amaranth plants with Agrobacterium tumefaciens strain GV3101 carrying pCBV19 gene vector that contained bar and gus genes, transgenic seeds were obtained. The functioning the tran+sferred genes in Amaranthus tissues was confirmed herbicide selection (PPT – phospinotricin) activity. Positive results obtained for cultivars “Karmin” “Kremoviy rannii”. percentage GUS positive samples 1% (for “Karmin”), 2.2% rannii”) from total initial quantity prior to...

BACKGROUND:Amelanchier alnifolia is an attractive small fruit difficult to propagate by traditional methods. Moreover, this species can be susceptible dormancy after transplanting the soil. OBJECTIVE:The aim of work was evaluate factors that contribute effective in vitro rooting d acclimatization micropropagated shoots Amelanchier alnifolia. METHODS:Different auxins, media concentrations, and sprays containing plant growth regulators were tested. The experimental data treated analysis...

Methodological Approaches to Simple Enzyme Polymorphism Analyses of Amaranth Species ( Amaranthus SP.) Amaranths have drawn a great interest in last years as an agronomic crop, alternative energy source and ornamental plants around the world. Several them are cultivated leaf vegetables, cereals, or colourful, quick growing garden plants. Increasing demand for breeding proteomic study species led us test some methodological approaches enzyme polymorphism analysis. For these experiments...

Murashige and Skoog medium (MS) modified Anderson’s Rhododendron (mAN) were compared for in vitro shoot multiplication of three highbush blueberries ‘Berkeley’, ‘Bluecrop’ ‘Goldtraube’. All media contained 0.5 mg/L zeatin applied either alone or combined with 0.1, 1 5 IBA. In rooting was induced using mAN supplemented 0.8 IBA 4 g/L activated charcoal. The results obtained showed that is more suitable the selected blueberry cultivars than MS medium. Low concentration (≤1 mg/L) added...

Possibilities of adventitious buds induction on the cotyledons obtained from sterile seedlings ofAbies concolor xAbies grandis hybrid were investigated. The following variables influencing bud and their further development studied: effect expiant age, different growth regulators concentrations duration application. most suitable expiants proved to be 7 d old seedlings. efficient cytokinin was benzylaminopurine (S mg l-1) in combination with napthaleneacetic acid (0.01 l-1). optimal treatment...

The aim of this work was to assess the regeneration capacity Amelanchier alnifolia var. cusickii and Lonicera kamtschatica cv. ‘Jugana’ from different types explants under various hormonal treatments. whole leaves, petioles, internodal segments in vitro plants were examined as explants. Several plant growth regulators (cytokinins auxins) evaluated for their ability induce adventitious regeneration. Direct indirect organogenesis achieved certain culture conditions both species. frequency...