A5005305852- Cancer-related molecular mechanisms research
- Ruminant Nutrition and Digestive Physiology
- Meat and Animal Product Quality
- Reproductive Physiology in Livestock
- Extracellular vesicles in disease
- Liver Disease Diagnosis and Treatment
- Genetic and phenotypic traits in livestock
- Adipose Tissue and Metabolism
- Milk Quality and Mastitis in Dairy Cows
- Animal Nutrition and Physiology
- Adipokines, Inflammation, and Metabolic Diseases
- MicroRNA in disease regulation
- Food Industry and Aquatic Biology
- Fatty Acid Research and Health
- Birth, Development, and Health
- Probiotics and Fermented Foods
- Organ Transplantation Techniques and Outcomes
- Pharmacological Effects and Assays
- Cardiovascular Disease and Adiposity
- Pluripotent Stem Cells Research
- Exercise and Physiological Responses
- Educational Environments and Student Outcomes
- Aquaculture disease management and microbiota
- Indigenous Studies and Ecology
- Pharmaceutical and Antibiotic Environmental Impacts
University of Vermont
2019-2023
Washington State University
2017-2018
Iowa State University
2005-2017
Abstract Adipose tissue‐derived mesenchymal stem cells (ASCs) from livestock are valuable resources for animal reproduction and veterinary therapeutics. Previous studies have shown that hypoxic conditions were beneficial in maintaining the physiological activities of ASCs. However, effects hypoxia on buffalo ASCs (bASCs) remain unclear. In this study, proliferation, stemness, reprogramming into induced pluripotent (iPSCs) bASCs examined. The results showed culture (5% oxygen) enhanced...
Abstract Background Bovine milk contains extracellular vesicles (EVs) that play a role in cellular communication, acting either an autocrine, paracrine, or exocrine manner. The unique properties of the EVs protect cargo against degradation. We profiled ncRNAs (non-coding RNA) present from seven dairy products - raw whole milk, heat-treated skim homogenized pasteurized heavy whipping cream, sweet cream buttermilk and cultured with four replicates each, obtained at different processing steps...
Abstract Background: Bovine milk contains extracellular vesicles (EVs) that play a role in cellular communication, acting either an autocrine, paracrine, or exocrine manner. The unique properties of the EVs protect cargo against degradation. We profiled ncRNAs (non-coding RNA) present from five fluid dairy products - raw whole milk, heat-treated skim homogenized pasteurized and heavy whipping cream (four replicates each) obtained at different processing steps commercial plant. their were...
Reduced-fat dried distillers’ grains with solubles (RF-DDGSs) are co-products of ethanol production and contain less fat than traditional grains. The in corn is ~91% unsaturated, it toxic to rumen microorganisms so could influence the composition microbiome. It has been demonstrated that RF-DDGS a suitable ration ingredient support high-producing dairy cow, this feedstuff promising alternative protein source for lactating cows. current study aims better understand effect on fecal bacterial...
Our objectives were to investigate fatty acid composition variation amongst adipose tissue sites, breed effects on fat quality, and the relationship of pork quality fresh quality. Barrows gilts (n = 347) five purebred one commercial crossbred line fed swine diets with DDGS inclusion at 30% (as fed) from 31.8 kg body weight until 30-d prior harvest 111.4 kg. Immediately after harvest, hot carcass was determined, collected back, belly, jowl, meat samples taken longissimus muscle for evaluation...
Adipocyte sizes from adipose tissue of mature animals form a bimodal distribution, thus reporting mean cell size is misleading. The objectives this study were to develop robust method for testing bimodality porcine adipocytes, describe the distribution with an informative metric, and statistically test hypertrophy appearance new small possibly resulting hyperplasia or lipid filling previously divided fibroblastic cells. Ninety-three percent samples measured (P < 0.0001); therefore, we...
Hepatic lipidosis (i.e., fatty liver) is a common periparturient disease in high-producing dairy cattle affecting nearly 50% of cows to some degree and costing an estimated 60 million dollars annually. Large animal studies are costly, labor intensive, not well suited mechanistic studies. Traditionally, employ vitro methodologies, utilizing established cell lines or primary culture methods. However, with cattle, hepatic do exist, methods for typically involve complicated procedures that often...
Fatty liver disease (FLD) is a major metabolic disorder of over‐conditioned periparturient dairy cattle and can precede many costly comorbidities, such as mastitis ketosis. While management practices decrease the incidence rate FLD, etiology not fully understood. In vivo studies using large animals are expensive, difficult to perform, time‐consuming; so, an in vitro model FLD would be valuable for probing biochemical mechanisms disease. Available methods isolation bovine cells mostly...
Mitochondrial complex I inhibitor (iC1) is a methylation‐controlled J protein that decreases oxidative phosphorylation of cellular respiration. Recent rodent studies showed loss or inhibition iC1 was associated with prevention lipid accumulation and T‐cell survival. One the common metabolic disorder dairy cattle fatty liver disease (FLD), which often occurs during periparturient period. Thus far, evidence iC1expression in bovine its possible role FLD development are not known. We...
Adipocyte sizes from adult adipose tissue are distributed bimodally. Thus, the standard metric reported, mean cell size, could be misleading. The objectives of this study were to develop a novel method for testing bimodality adipocytes in porcine tissue, describe size distribution with more informative metric, and statistically test hypertrophy hyperplasia. Testing was done by describing two bins bin 1 (adipocytes 25.0 - 40.1 µm diameter) containing suspected minor mode 2 50.2 65.4 region...
Mitochondrial complex I inhibitor (iC1) is a methylation-controlled J protein (MCJ) that decreases cellular respiration by inhibiting oxidative phosphorylation. Recent rodent studies showed loss or inhibition of iC1 was associated with preventing lipid accumulation. A common metabolic disorder dairy cattle fatty liver disease (FLD), which often occurs during the periparturient period. In humans and rodents, expressed in acts as mitochondrial "brake". However, expression bovine its possible...