A5078553081- Phytoplasmas and Hemiptera pathogens
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Plant Pathogenic Bacteria Studies
- Insect Resistance and Genetics
- Cocoa and Sweet Potato Agronomy
- Plant Virus Research Studies
- Hymenoptera taxonomy and phylogeny
- Mosquito-borne diseases and control
- Research on scale insects
- Studies on Chitinases and Chitosanases
- Entomopathogenic Microorganisms in Pest Control
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Horticultural and Viticultural Research
- Plant pathogens and resistance mechanisms
- Genomics and Phylogenetic Studies
- Insect and Pesticide Research
- Molecular Biology Techniques and Applications
- Photosynthetic Processes and Mechanisms
- bioluminescence and chemiluminescence research
- melanin and skin pigmentation
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Cultural Heritage Materials Analysis
National Research Council
2012-2024
Institute for Sustainable Plant Protection
2015-2024
Consorzio Roma Ricerche
2024
Centro di Ricerca per la Patologia Vegetale
2011-2016
University of Turin
2005-2013
Oklahoma State University
2008
Phytoplasmas, uncultivable phloem-limited phytopathogenic wall-less bacteria, represent a major threat to agriculture worldwide. They are transmitted in persistent, propagative manner by phloem-sucking Hemipteran insects. Phytoplasma membrane proteins direct contact with hosts and presumably involved determining vector specificity. Such role has been proposed for phytoplasma transmembrane encoded circular extrachromosomal elements, at least one of which is plasmid. Little known about the...
ou non, émanant des établissements d'enseignement et de recherche français étrangers, laboratoires publics privés.
Abstract Three real‐time PCR–based assays for the specific diagnosis of flavescence dorée (FD), bois noir (BN) and apple proliferation (AP) phytoplasmas a universal one detection belonging to groups 16Sr‐V, 16Sr‐X 16Sr‐XII have been developed. Ribosomal‐based primers CYS2Fw/Rv TaqMan probe CYS2 were used in PCR. For group‐specific FD phytoplasma, ribosomal‐based fAY/rEY, 16Sr‐V phytoplasmas, chosen. BN AP designed on non‐ribosomal nitroreductase DNA sequences, respectively. SYBR ® Green I...
Flavescence dorée phytoplasma ( FDP ) titre in two red grapevine cultivars, B arbera and N ebbiolo, was measured over the vegetative seasons of consecutive years vineyards P iemonte R egion (northwestern I taly), with a double absolute quantification cells DNA real‐time PCR . The relationships pathogen concentration to cultivar susceptibility symptom severity were investigated. always higher cv. Barbera than ebbiolo infected vines, this difference significant at early late summer samplings...
Phytoplasmas are bacterial plant pathogens (class Mollicutes), transmitted by phloem feeding leafhoppers, planthoppers and psyllids in a persistent/propagative manner. Transmission of phytoplasmas is under the control behavioral, environmental geographical factors, but molecular interactions between membrane proteins phytoplasma vectors may also be involved. The aim work was to provide experimental evidence that vivo interaction antigenic protein (Amp) vector has role transmission process....
ABSTRACT Phytoplasmas are plant-pathogenic bacteria transmitted by hemipteran insects. The leafhopper Euscelidius variegatus is a natural vector of chrysanthemum yellows phytoplasma (CYp) and laboratory flavescence dorée (FDp). two phytoplasmas induce different effects on this species: CYp slightly improves whereas FDp negatively affects insect fitness. To investigate the molecular bases these responses, transcriptome sequencing (RNA-seq) analysis E. infected with either or was performed....
Flavescence dorée is an epidemic yellows disease of grapevine, caused by a phytoplasma (FDP), for which there currently no cure. We assessed whether the endophyte Pseudomonas migulae 8R6, able to synthesize 1-aminocyclopropane-1-carboxylate (ACC) deaminase, can limit phytoplasma-induced damages in periwinkle, model plant hosting FDP. Plant protection induced 8R6 and its mutant, impaired ACC deaminase synthesis, was compared. Fifteen plants per treatment were used; FD infection transmitted...
The titer of chrysanthemum yellows phytoplasma (CYP, “Candidatus Phytoplasma asteris”) in the three vector species Euscelis incisus Kirschbaum, Euscelidius variegatus and Macrosteles quadripunctulatus Kirschbaum (Homoptera: Cicadellidae) was measured after controlled acquisition from infected Chrysanthemum carinatum (Schousboe) (daisy) plants. DNA quantified relation to insect (genome units [GU] per ng DNA) by using a quantitative real-time polymerase chain reaction (PCR) procedure. increase...
Journal Article Host Plant Determines the Phytoplasma Transmission Competence of Empoasca decipiens (Hemiptera: Cicadellidae) Get access L. Galetto, Galetto Search for other works by this author on: Oxford Academic PubMed Google Scholar C. Marzachì, Marzachì S. Demichelis, Demichelis D. Bosco 3 3Corresponding author, e-mail: domenico.bosco@unito.it Economic Entomology, Volume 104, Issue 2, 1 April 2011, Pages 360–366, https://doi.org/10.1603/EC10174 Published: 01 2011 history Received: 12...
The immunodominant membrane protein Imp of several phytoplasmas within the ‘ Candidatus Phytoplasma aurantifolia’ (16Sr‐II) group was investigated. Eighteen isolates from Iran (11), East Asia (5), Africa (1) and Australia clustered into three phylogenetic subgroups (A, B C) based on 16S rDNA imp genes, regardless geographic origin. gene sequences were variable, with more non‐synonymous than synonymous mutations (68 vs 20, respectively), even though many ones (75%) produced conservative amino...
Abstract Phytoplasmas are wall‐less bacteria associated with many plant diseases of wild and cultivated plants, transmitted by hemipteran insects. In nature, vectors can acquire more than one phytoplasma feeding on multiple‐infected source plants or sequentially infected different phytoplasmas. The acquisition multiple phytoplasmas may lead to their interaction in the this work, nymphs leafhopper Euscelidius variegatus were allowed feed Flavescence dorée (FDP)‐infected chrysanthemum yellows...
The phytoplasma-borne disease flavescence dorée is still a threat to European viticulture, despite mandatory control measures and prophylaxis against the leafhopper vector. Given economic importance of grapevine, it essential find alternative strategies contain spread, in order possibly reduce current use harmful insecticides. Further studies pathogen, vector mechanisms phytoplasma-host interactions could improve our understanding disease. In this work, RNA-Seq technology followed by three...
ABSTRACT To highlight different transcriptional behaviors of the phytoplasma in plant and animal host, expression 14 genes “ Candidatus Phytoplasma asteris,” chrysanthemum yellows strain, was investigated at times following infection a host ( Arabidopsis thaliana ) two insect vector species Macrosteles quadripunctulatus Euscelidius variegatus ). Target were selected among those encoding antigenic membrane proteins, transporters, secreted general enzymes. Transcripts detected for all analyzed...
In this study, binding between the immunodominant membrane protein Imp of 16SrV‐D phytoplasma associated with Flavescence dorée disease (FD‐Dp) and insect proteins vectors non‐vectors FD‐Dp was tested. Six Auchenorrhyncha species, from distantly related groups were selected: Scaphoideus titanus, Euscelidius variegatus, Macrosteles quadripunctulatus, Zyginidia pullula (Cicadomorpha), Ricania speculum Metcalfa pruinosa (Fulgoromorpha). The vector status each species retrieved literature or...
Abstract Phytoplasmas are plant‐pathogenic Mollicutes transmitted by leafhoppers, planthoppers, and psyllids in a persistent propagative manner. Chrysanthemum yellows phytoplasma (CY) is member of ‘ Candidatus Phytoplasma asteris’, 16Sr‐IB, at least three leafhopper species, Macrosteles quadripunctulatus Kirschbaum, Euscelidius variegatus Euscelis incisus Kirschbaum (all Homoptera: Cicadellidae: Deltocephalinae). Although M. transmits CY with very high efficiency (near 100%), 25% E....
Abstract Flavescence dorée ( FD ) is among the major grapevine diseases causing high management costs; curative methods against are unavailable. In ‐infected plants, decrease in photosynthesis usually recorded, but deregulation stomatal control of leaf gas exchange during infection and recovery unknown. We measured seasonal time course rates two cultivars (‘Barbera’ ‘Nebbiolo’) term 1 year when grapevines experienced a water stress another with no drought, difference response to as assessed...
Analyses of space-time statistical features a flavescence dorée (FD) epidemic in
Flavescence dorée (FD) is a threat for wine production in the vineyard landscape of Piemonte, Langhe-Roero and Monferrato, Italy. Spread disease dependent on complex interactions between insect, plant phytoplasma. In Piemonte region, based local cultivars. The role six grapevine varieties as source inoculum vector Scaphoideus titanus was investigated. FD phytoplasma (FDP) load compared among red white with different susceptibility to FD. Laboratory-reared healthy S. nymphs were caged...
The leafhopper Scaphoideus titanus is the primary vector of Flavescence dorée phytoplasma (FDp) in European vineyards. one most severely damaging diseases Vitis vinifera and, consequently, a major threat to grape and wine production several countries. Control measures are compulsory, but they mainly involve large-scale insecticide treatments, with detrimental impacts on environment. One possible solution exploit largely unexplored genetic diversity viruses infecting S. as highly specific...
Halyomorpha halys (Stål), the brown marmorated stink bug, is a highly invasive insect species that causes significant agricultural losses, especially to orchard fruits, vegetables, herbaceous and ornamental plants. It also nuisance pest seeks shelter in indoor spaces during winter months. Harnessing H. virome can result new environmentally sustainable approaches contain its populations relatated damages. In this study, RNA-Seq data were used explore associated ten field collected Lombardy...
RNA interference (RNAi) techniques have emerged as powerful tools to develop novel management strategies for the control of insect pests. The leafhopper Euscelidius variegatus is a natural vector chrysanthemum yellows phytoplasma and laboratory Flavescence dorée phytoplasma. Phytoplasmas are insect-borne bacterial plant pathogens that cause economically relevant crop losses worldwide.In this study, we demonstrated microinjection muscle actin ATP synthase β double-stranded (ds)RNAs into adult...
A set of experiments was carried out to characterize how temperature affects the spread chrysanthemum yellows phytoplasma (CYP), a strain Candidatus Phytoplasma asteris, in Chrysanthemum carinatum plants transmitted by Macrosteles quadripunctulatus leafhopper. Experiments provided data on CYP latency period insect and plant host, M. adult mortality rate, epidemics progression under controlled conditions inside climatic chambers. were conducted at temperatures ranging between 15 30°C....