A5090529271- Aquaculture disease management and microbiota
- interferon and immune responses
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Viral Infections and Vectors
- Immune Cell Function and Interaction
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
- Cytokine Signaling Pathways and Interactions
- Toxoplasma gondii Research Studies
- Platelet Disorders and Treatments
- Autophagy in Disease and Therapy
- Neurobiology and Insect Physiology Research
- Lipid Membrane Structure and Behavior
- Cancer-related molecular mechanisms research
- Animal Virus Infections Studies
- Immune cells in cancer
- Complement system in diseases
- MicroRNA in disease regulation
- Signaling Pathways in Disease
- RNA Interference and Gene Delivery
- Chemokine receptors and signaling
- Viral Infections and Outbreaks Research
- Zebrafish Biomedical Research Applications
- Iron Metabolism and Disorders
The Ohio State University
2025
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2025
Huazhong Agricultural University
2016-2024
Qingdao National Laboratory for Marine Science and Technology
2018-2024
Abstract TLRs are pivotal pattern recognition receptors in initiating innate immunity and triggering adaptive immunity. TLR pathways have been comprehensively investigated mammals. However, the teleost-specific TLR19 pathway remains largely unknown. In this study, we identified from grass carp (Ctenopharyngodon idella), explored ligand, adaptor, signaling pathways. Pathogen-associated molecular binding luciferase activity assays indicate that recognizes responds to dsRNA analog...
In mammals, TLR5 functions as a homodimer to recognize bacterial flagellin on the cytomembrane. The current investigations reveal existence of two types TLR5, membrane-bound PmTLR5M, and soluble variant PmTLR5S, in lamprey (Petromyzon marinus). Although both PmTLR5M PmTLR5S can bind flagellin, only is capable eliciting proinflammatory response, whereas detect facilitate role early endosomes. trafficking chaperone UNC93B1 enhances ligand-induced signaling via or combination PmTLR5S. recruits...
Autophagy plays many physiological and pathophysiological roles. However, the roles regulatory mechanisms of autophagy in response to viral infections are poorly defined teleost fish, such as grass carp (Ctenopharyngodon idella), which is one most important aquaculture species China. In this study, we found that both reovirus (GCRV) infection hydrogen peroxide (H2O2) treatment induced accumulation reactive oxygen (ROS) C. idella kidney cells stimulate autophagy. Suppressing ROS with...
Abstract Type I IFNs (IFN-Is) play pivotal roles in host defense against viral infections but remain enigmatic bacterial pathogens. In this study, we recombinantly expressed and purified intact grass carp (Ctenopharyngodon idella) IFNφ1 (gcIFNφ1), a teleost IFN-I. gcIFNφ1 widely powerfully directly kills both Gram-negative Gram-positive bacteria dose-dependent manner. binds to LPS or peptidoglycan provokes membrane depolarization disruption, resulting death. Furthermore, can efficiently...
Grass carp reovirus (GCRV) is highly infectious and lethal to grass carp, causing huge economic losses the aquaculture industry annually. Currently, vaccination most effective method against viral infections. Among various methods, oral an ideal way in aquaculture. However, low protective efficiency major problem for owing some reasons, such as antigen degradation immunogenicity. In our study, we screened antigenic epitopes of GCRV-II prepared microencapsulated vaccine using sodium alginate...
Abstract Type I IFNs with strong positive charges exhibit robust bactericidal activity and a protective effect against bacterial infections. However, the antibacterial mechanism in vivo remains unknown. In this study, Ab blockade of IFN1, member type grass carp (Ctenopharyngodon idella), resulted high mortality, tissue loads, low expression immune factors after challenge, which indicates that IFN1 has physiological significance. Meanwhile, we injected recombinant purified intact protein...
Optimal activation of CD8+ T cells is crucial for immunity-mediated destruction cancer, requiring a substantial amount proteins involved in metabolism, proliferation, and effector function. Despite extensive studies emphasizing the role transcriptional regulation this process, paired transcriptomic proteomic analyses reveal that RNA profile poorly correlated with protein levels. This discrepancy underscores importance post-translational modifications (PTMs) controlling abundance during...
Retinoic acid-inducible gene I (RIG-I) and melanoma differentiation associated 5 (MDA5) are critical cytosolic sensors that trigger the production of interferons (IFNs). Though their recognition functions well identified, unique roles in downstream signal transduction remain to be elucidated. Herein, we report differential effect between grass carp (Ctenopharyngodon idella) MDA5 (CiMDA5) CiRIG-I on various IFNs upon reovirus (GCRV) infection C. idella kidney (CIK) cell line. In CIK cells,...
Abstract The major role of chemokines is to act as a chemoattractant guide the migration immune cells infectious sites. In current study, we found that CiCXCL20a, teleost-specific chemokine from grass carp (Ctenopharyngodon idella), demonstrates broad-spectrum, potent, direct bactericidal activity and immunomodulatory functions bacterial infections, apart chemotaxis. CiCXCL20a kills bacteria by binding, mainly targeting acid lipids, perforating membrane, resulting in cytoplasm leakage death....
Grass carp reovirus (GCRV) causes extensive infection and death in grass black fingerlings, with a highly seasonal prevalence. Previous studies suggested that GCRV can become latent after primary infection. In this study, we investigated type II (GCRV-II) latency asymptomatic or exposure history. We found during infection, GCRV-II was detectable only the brain of carp, unlike multi-tissue distribution observed natural caused damage to while brain, heart, eye tissues had relatively higher...
Macrophages are very versatile immune cells, with the characteristics of a proinflammatory phenotype in response to pathogen-associated molecular patterns. However, specific activation marker genes macrophages have not been systematically investigated teleosts. In this work, leukocytes (WBC) were isolated using Percoll gradient method. enriched by adherent culture WBC, then stimulated lipopolysaccharide (LPS). identified morphological features, functional activity and authorized cytokine...
TLR22 exists in nearly all the poikilothermic vertebrates, playing a central role initiation of innate immunity and activation adaptive immunity. signaling pathway has been characterized fugu (Takifugu rubripes) detail. Here, we thoroughly remould localization pathways TLR22. We employed TLR22a TLR22b grass carp (Ctenopharyngodon idella), designated as CiTLR22a CiTLR22b, explored ligand(s), adaptor(s) pathway(s). Confocal fluorescence microscopy demonstrates that both CiTLR22b localize to...
TLR7 plays a crucial role in sensing viral ssRNA and initiating immune responses. Piscine also responds to dsRNA challenge. exists almost all the viruses at specific stages. However, mechanism on by remains unknown. In present study, we employed Ctenopharyngodon idella (CiTLR7) systematically explore functions mechanisms teleost. CiTLR7 can directly bind not only but different patches lysosome, recruit MyD88 as adaptor, activate downstream IFN pathway via SLC15A4/TASLa/TASLb/IRF5/IRF7...
Abstract Grass carp ( Ctenopharyngodon idella ) is an important economic species in freshwater aquaculture and its industry has been confined due to variety degeneration frequent diseases. Marker-assisted selection a feasible method for selective breeding of new varieties. Transcriptome data have greatly facilitated high-throughput single nucleotide polymorphism (SNP) marker discovery phenotype association study. In this study, we gained total 25,981 5,775 high quality SNPs two...
// Hang Su 1, 2 , Zhiwei Liao Gailing Yuan and Jianguo 2, 3 1 College of Fisheries, Huazhong Agricultural University, Wuhan, China Hubei Engineering Technology Research Center for Aquatic Animal Disease Control Prevention, Laboratory Marine Biology Biotechnology, Qingdao National Science Technology, Qingdao, Correspondence to: Su, email: sujianguo@mail.hzau.edu.cn Keywords: grass carp ( Ctenopharyngodon idella ), plasmid containing CpG ODNs, reovirus, adjuvant, immune protection Received:...
Diseases caused by viruses threaten the production industry and food safety of aquaculture which is a great animal protein source. Grass carp reovirus (GCRV) has tremendous loss, molecular function viral proteins during infection needs further research, as for most aquatic viruses. In this study, interaction between GCRV major outer capsid VP4 RIG-I, critical RNA sensor, was screened out GST pull-down, endogenous immunoprecipitation subsequent LC-MS/MS, then verified co-IP an advanced...
Grass carp reovirus (GCRV), the most virulent aquareovirus, causes epidemic hemorrhagic disease and tremendous economic loss in freshwater aquaculture industry. VP56, a putative fibrin inlaying outer surface of GCRV-II GCRV-III, is involved cell attachment. In present study, we found that VP56 localizes at early endosome, lysosome, endoplasmic reticulum, recruits cytoplasmic viral RNA sensor retinoic acid-inducible gene I (RIG-I) binds to it. The interaction between RIG-I was detected by...