A5013411151- Vector-borne infectious diseases
- Ion Channels and Receptors
- Viral Infections and Vectors
- Connexins and lens biology
- Vector-Borne Animal Diseases
- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Insect symbiosis and bacterial influences
- Diagnosis and Treatment of Venous Diseases
- Photochromic and Fluorescence Chemistry
- Neurobiology and Insect Physiology Research
- Advanced Glycation End Products research
- Pain Mechanisms and Treatments
- Ocular Surface and Contact Lens
- Glaucoma and retinal disorders
- Neuroscience and Neural Engineering
- Mosquito-borne diseases and control
University at Albany, State University of New York
2019-2022
Wadsworth Center
2019-2020
New York State Department of Health
2019-2020
University of Utah
2012-2016
Neural Stem Cell Institute
2016
Abstract An intractable challenge in glaucoma treatment has been to identify druggable targets within the conventional aqueous humor outflow pathway, which is thought be regulated/dysregulated by elusive mechanosensitive protein(s). Here, biochemical and functional analyses localized putative cation channel TRPV4 plasma membrane of primary immortalized human TM (hTM) cells, mouse tissue. Selective agonists substrate stretch evoked TRPV4-dependent cation/Ca 2+ influx, thickening F-actin...
Activity-dependent shifts in ionic concentrations and water that accompany neuronal glial activity can generate osmotic forces with biological consequences for brain physiology. Active regulation of gradients cellular volume requires volume-sensitive ion channels. In the vertebrate retina, critical support to is provided by Müller astroglia, but identity their osmosensor unknown. Here, we identify TRPV4 channels as transducers mouse cell increases into physiological responses. Hypotonic...
Significance The secretion of aqueous humor from the ciliary body is regulated by osmotic gradients, yet mechanism through which these cells sense gradients still under debate. We have identified calcium-permeable transient receptor potential vanilloid isoform 4 (TRPV4) ion channel as critical for translating hypotonic stimuli into intracellular signals and linked activation this to a known proinflammatory lipid signaling pathway. was confined nonpigmented that secrete fluid regulate...
Numerous preclinical studies have shown that transplantation of stem cell-derived retinal pigment epithelial cell (RPE) preserves photoreceptor anatomy in the dystrophic Royal College Surgeons (RCS) rat. How rescue is spatially distributed over eye, relative to site, less clear. To understand spatial variations transplant efficacy, we developed a method measure distribution rescued cells.Human RPE Stem Cell-derived (RPESC-RPE) cells were subretinally injected into RCS rat eyes. After tissue...
Lyme disease vaccines based on recombinant Outer surface protein A (OspA) elicit protective antibodies that interfere with tick-to-host transmission of the disease-causing spirochete Borreliella burgdorferi. Another hallmark OspA antisera and certain monoclonal (MAbs) is their capacity to induce B. burgdorferi agglutination in vitro, a phenomenon first reported more than 30 years ago but never studied molecular detail. In this report, we demonstrate transmission-blocking MAbs, individually...