A5021101856- Protein Structure and Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Hemoglobin structure and function
- Enzyme Catalysis and Immobilization
- Origins and Evolution of Life
- Mass Spectrometry Techniques and Applications
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- Photosynthetic Processes and Mechanisms
- Genetics, Bioinformatics, and Biomedical Research
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Metabolomics and Mass Spectrometry Studies
- Gene Regulatory Network Analysis
- Analytical Chemistry and Chromatography
- Mitochondrial Function and Pathology
- Enzyme Structure and Function
- Cancer, Hypoxia, and Metabolism
- Computational Drug Discovery Methods
- Bioinformatics and Genomic Networks
- RNA and protein synthesis mechanisms
- Enzyme function and inhibition
- thermodynamics and calorimetric analyses
- Biochemical Acid Research Studies
- Biotechnology and Related Fields
Bioénergétique et Ingénierie des Protéines
2008-2023
Aix-Marseille Université
2013-2023
Centre National de la Recherche Scientifique
2013-2023
Université Joseph Fourier
2020
Délégation Provence et Corse
1999-2017
Institute of Molecular Biology and Biophysics
2016
Institut de Microbiologie de la Méditerranée
2010-2014
Heidelberg University
2013
Centre National pour la Recherche Scientifique et Technique (CNRST)
1987-2013
Dean College
2013
Abstract Motivation: Molecular biotechnology now makes it possible to build elaborate systems models, but the biology community needs information standards if models are be shared, evaluated and developed cooperatively. Results: We summarize Systems Biology Markup Language (SBML) Level 1, a free, open, XML-based format for representing biochemical reaction networks. SBML is software-independent language describing common research in many areas of computational biology, including cell...
A new plot is described for analysing the results of kinetic experiments in which Michaelis-Menten equation obeyed. Observations are plotted as lines parameter space, instead points observation space. With appropriate modifications applicable to most problems interest enzyme kineticist. It has following advantages over traditional methods plotting results: it very simple construct, because composed entirely straight and requires no calculation or mathematical tables; constants read off...
A new method is described for plotting kinetic results inhibited enzyme-catalysed reactions. It provides a simple way of determining the inhibition constant, K′i, an uncompetitive, mixed or non-competitive inhibitor.
Journal Article Nomenclature for incompletely specified bases in nucleic acid sequences: rcommendations 1984 Get access Athel Cornish-Bowden Department of Biochemistry, University BirminghamP.O. Box 363, Birmingham B15 2TT, UK * As secretary the Committee International Union which thanks a panel convened by Dr Richard Lathe drafting these recommendations. The members committee and are given Acknowledgements. Search other works this author on: Oxford Academic PubMed Google Scholar Nucleic...
The statistical implications of the direct linear plot for enzyme kinetic data, described in preceding paper (Eisenthal & Cornish-Bowden, 1974), are discussed case Michaelis-Menten equation. is shown to lead directly non-parametric confidence limits parameters, V and K(m), which depend on far less sweeping assumptions about nature experimental error than those implicit method least squares. Median estimates K(m) can also be defined, more robust least-squares a wide variety situations.
1. A simple method is described for calculating the free concentrations of all species in a mixture several ionic components that associate at equilibrium to any extent and with stoicheiometry. 2. It can readily be adapted take account such as protons which rather than total are controlled. 3. was applied mixtures adenine nucleotides, Mg2+ other ions relevant study glucokinase (EC 2.7.1.2), but qualitative conclusions not peculiar this system. 4. ATP exists high nearly constant proportion...
The year 2013 marked the centenary of paper Leonor Michaelis and Maud Menten (Michaelis Menten, 1913), 110th anniversary doctoral thesis Victor Henri (Henri, 1903). These publications have had an enormous influence on progress biochemistry, are more often cited in 21st century than they were 20th. laid groundwork for understanding enzyme mechanisms, but his experimental design was open to criticism. He reached essentially correct conclusions about action invertase, he took no steps control...
Enzyme kinetics Uncompetitive inhibition Catastrophic response Pesticide design
amino acids. 13.4 Numbering of peptide atoms.13.5 Prefixes formed from names ..
1. A theory is developed to account for the kinetics of coupled-enzyme reactions without assuming that second reaction follows first-order kinetics. 2. simple procedure described applying practical design enzyme assays. 3. The validity confirmed assay glucokinase with glucose 6-phosphate dehydrogenase as coupling enzyme. 4. possibility extending three or more coupled discussed.
The kinetics of glucokinase from rat liver were studied over wide ranges glucose and MgATP2- concentrations. initial rate shows a co-operative dependence on the concentration, with Hill coefficients in range 1.2-1.5. degree co-operativity increases but no was detected for concentration. effects observed occur at physiologically reasonable concentrations are consistent presumed function maintaining constant concentration blood.
Inhibition studies of glucokinase were carried out with the products reaction, glucose 6-phosphate and MgADP-, as well ADP3-, Mg2+ ATP4-. The results these, together those kinetic uninhibited reaction described previously [Storer & Cornish-Bowden (1976) Biochem. J. 159, 7-14], indicate that enzyme obeys a 'mnemonical' mechanism. This implies co-operativity observed substrate arises because binds differentially to two forms free are not in equilibrium under steady-state conditions. mechanism...
The International Union of Biochemistry (IUB, now IUBMB) prepared recommendations for describing the kinetic behaviour enzymes in 1981. Despite more than 30 years that have passed since these not subsequently been revised, though various respects they do adequately cover current needs. IUBMB is also responsible on naming and classification enzymes. In contrast to case kinetics, are kept continuously up date.