Abstract Core/shell nanoparticles that display a pH‐sensitive thermal response, self‐assembled from the amphiphilic tercopolymer, poly( N ‐isopropylacrylamide‐ co‐N , ‐dimethylacrylamide‐ co ‐10‐undecenoic acid) (P(NIPAAm‐ ‐DMAAm‐ ‐UA)), have recently been reported. In this study, folic acid is conjugated to hydrophilic segment of polymer through free amine group (for targeting cancer cells overexpress folate receptors) and cholesterol grafted hydrophobic polymer. This also self‐assembles...

A liquid marble micro‐bioreactor is prepared by placing a drop of murine embryonic stem cell (ESC) (Oct4B2‐ESC) suspension onto polytetrafluoroethylene (PTFE) particle bed. The Oct4B2‐ESC aggregates to form embryoid bodies (EBs) with relatively uniform size and shape in within 3 d. For the first time, feasibility differentiating ESC into cardiac lineages marbles being investigated. Without addition growth factors, suspended EBs from express various precardiac mesoderm markers including...

Treatment of surfaces to change the interaction fluids with them is a critical step in constructing useful microfluidics devices, especially those used biological applications. Silanization, generic term applied formation organosilane monolayers on substrates, both widely reported literature and troublesome actual application for uninitiated. These can be subsequently modified produce surface specific functionality. Here various deposition protocols some notes are provided as basis novice...

The confined internal space of a liquid marble, as well its porous and non-adhesive shell, offers an attractive application possibility - accommodating living cells inside marbles. Cancer in suspension may aggregate to form three dimensional structures, also known cancer cell spheroids (CCS). In this study, CCS formation marble is investigated. This opens significant novel avenues for biomedical applications research.

The encapsulation of therapeutic molecules within multiple layers biocompatible and biodegradable polymeric excipients allows exquisite design their release profile, to the extent drug can be selectively delivered a specific target location in vivo. Here, we develop novel technique for assembly multilayer polyelectrolyte nanocarriers based on surface acoustic wave atomization as rapid efficient alternative conventional layer-by-layer assembly, which requires use sacrificial colloidal...

This study describes the preparation and characterization of a biodegradable 3D hydrogel constructed from hydroxypropyl cellulose (HPC), modified with bifunctional methacrylic anhydride (MA) to form methacrylate (HPC-MA), for adipose tissue engineering applications. The hydrogels were prepared three different concentrations (10 wt%, 15 wt% 20 wt%) HPC-MA 0.35 degree substitution. scaffolds open biphasic features by exploiting thermal responsive phase behavior HPC temperature mediated...

Intense acoustically driven microcentrifugation flows are employed to enhance the assembly of cellular spheroids in microwell a tissue culture well plate. This ability interface microfluidics with commonly used plasticware is significant advantage as it can potentially be parallelized for high throughput operation and allows existing analytical equipment designed fit current laboratory formats retained. The flow, induced coated low adhesive hydrogel, shown rapidly concentration cells into...

Pollen is bees' primary source of proteins. Using pollen substitutes could reduce colony losses in areas with limited floral resources. In this study, we compared the effects made from intact proteins versus free amino acids on bee survival, as well levels vitellogenin (vg), major royal jelly protein 1 (mrjp1), and deformed wing virus (DWV). Our study revealed no significant differences vg mRNA between bees-fed acid diets. However, mrjp1 were higher diets, suggesting that availability...

Pollen is bees’ primary source of proteins. Using pollen substitutes could reduce colony losses in areas with limited floral resources. In this study, we compared the effects made from intact proteins versus free amino acids on bee survival, as well levels vitellogenin (vg), major royal jelly protein 1 (mrjp1), and deformed wing virus (DWV). Our study revealed no significant differences vg mRNA between bees fed acid diets. However, mrjp1 were higher diets, suggesting that availability...

The significant and inherent limitations associated with commonly used methods of in vitro embryoid body (EB) formation motivate the development novel, facile, efficient reproducible techniques. In this study we report possibility using "liquid marbles" as facile micro bioreactors for EB formation. To exploit liquid marbles biological reactors, embryonic stem cells (ES cells) were inoculated into containing cell growth medium. Herein show how confined internal space a marble, along porous...

Since rates of tissue growth vary significantly between types, and also individuals due to differences in age, dietary intake, lifestyle‐related factors, engineering a scaffold system that is appropriate for personalized remains significant challenge. In this study, gelatin‐hydroxyphenylpropionic acid/carboxylmethylcellulose‐tyramine (Gtn‐HPA/CMC‐Tyr) porous hydrogel allows the pore structure scaffolds be altered vivo after implantation developed. Cross‐linking Gtn‐HPA/CMC‐Tyr hydrogels via...

Abstract A miniaturized centrifugal microfluidic platform for lab‐on‐a‐chip applications is presented. Unlike its macroscopic Lab‐on‐a‐CD counterpart, the miniature Lab‐on‐a‐Disc (miniLOAD) device does not require moving parts to drive rotation of disc, inexpensive, disposable, and significantly smaller, comprising a 10‐mm‐diameter SU‐8 disc fabricated through two‐step photolithography. The driven rotate using surface acoustic wave irradiation incident upon fluid coupling layer from pair...

Brain repair following disease and injury is very limited due to difficulties in recruiting mobilizing stem cells towards the lesion. More importantly, there a lack of structural trophic support maintain viability stem/progenitor present. This study investigates effectiveness an injectable gelatin-based hydrogel attracting neural progenitor (NPCs) from subventricular zone (SVZ) implant. Glial cell-line-derived neurotrophic factor (GDNF) encapsulated within porosity prevents glial scar...

Despite the promise of stem cell therapy for lung therapeutics and repair, there are few viable means directly delivering cells to locally target respiratory airways via inhalation. This is not surprising given significant challenges in aerosolising cells, particularly their susceptibility damage under large stresses involved nebulisation process. Here, we present promising results using a microfluidic acoustic platform that only low cost portable, but also its high MHz order frequencies...

A handful of work focused on improving the intrinsic low mechanical properties hydroxyapatite (HA) by various reinforcing agents. However, big challenge regarding is maintaining bioactivity. To address this issue, we report fabrication apatite-based composites incorporation alumina nanoparticles (n-Al2O3). Although numerous studies have used micron or submicron for hydroxyapatite, only few reports are available about use n-Al2O3. In study, spark plasma sintering (SPS) method was utilized to...

We present histological data from 21 post-mortem, adult human cases that indicate the neocortex on left planum temporale (secondary auditory cortex) is thinner but longer than right side. The volumes of and regions are approximately equal. Thus, cortex long thin short thick. fit excellently with previous studies volume, surface area, cytoarchitectonics, neuronal structures these areas. From we suggest hemispheric differences arise a so-called "balloon model" cortical development. In this...

Abstract The present study presents a new approach for evaluating in vitro cytotoxicity of nanoparticles. is based on American National Standard ISO 10993‐5. Hepatoma HepG2 and fibroblast NIH3T3 cell lines were incubated with nanoparticles, their associated extracts derived at 70 121°C. Nanoparticles proposed as potential biomedical imaging probes evaluated the basis detection metabolic activities cell‐morphology changes. In general, nanoparticles directly cells showed higher than extracts....