A5057266958- Retinal Development and Disorders
- High Altitude and Hypoxia
- Antiplatelet Therapy and Cardiovascular Diseases
- Retinal Diseases and Treatments
- Venous Thromboembolism Diagnosis and Management
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- Irrigation Practices and Water Management
- Photosynthetic Processes and Mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Endoplasmic Reticulum Stress and Disease
- Glaucoma and retinal disorders
- Genomics and Rare Diseases
- Histone Deacetylase Inhibitors Research
- Pluripotent Stem Cells Research
- Hydraulic flow and structures
- Cancer, Hypoxia, and Metabolism
- Quality and Safety in Healthcare
- HIV Research and Treatment
- Water resources management and optimization
- Bacillus and Francisella bacterial research
- Mitochondrial Function and Pathology
- Micro and Nano Robotics
- Genomic variations and chromosomal abnormalities
- Advanced biosensing and bioanalysis techniques
Genome Institute of Singapore
2020-2025
Agency for Science, Technology and Research
2023-2025
Yale University
2019-2024
University of Wisconsin–Madison
2023
All India Institute of Medical Sciences
2019-2022
Deen Dayal Upadhyay Hospital
2018-2022
SingHealth
2022
Duke-NUS Medical School
2022
University of Cologne
2020-2021
University Hospital Cologne
2018-2021
One approach to understanding the genetic basis of traits is study their pattern inheritance among offspring phenotypically different parents. Previously, such analysis has been limited by low mapping resolution, high labor costs, and large sample size requirements for detecting modest effects. Here, we present a novel map trait loci using artificial selection. First, generated populations 10–100 million haploid diploid segregants crossing two budding yeast strains heat tolerance up 12...
The synonymous substitution rate varies widely among species, but it is generally quite stable within a genome due to the absence of strong selective pressures. In plants, plastid genes tend evolve faster than mitochondrial genes, variation species correlates between and genomes, few examples intragenomic heterogeneity exist. To study extent plant organellar we sequenced complete genomes from bugleweed, Ajuga reptans, which was previously shown exhibit for several genes. Substitution rates...
A liquid marble micro‐bioreactor is prepared by placing a drop of murine embryonic stem cell (ESC) (Oct4B2‐ESC) suspension onto polytetrafluoroethylene (PTFE) particle bed. The Oct4B2‐ESC aggregates to form embryoid bodies (EBs) with relatively uniform size and shape in within 3 d. For the first time, feasibility differentiating ESC into cardiac lineages marbles being investigated. Without addition growth factors, suspended EBs from express various precardiac mesoderm markers including...
Asian populations are under-represented in human genomics research. Here, we characterize clinically significant genetic variation 9051 genomes representing East Asian, South and severely Austronesian-speaking Southeast ancestries. We observe disparate risk burden attributable to ancestry-specific recurrent variants identify individuals with specific ancestries discordant their self-reported ethnicity, mostly due cryptic admixture. About 27% of severe recessive disorder genes appreciable...
Horizontal gene transfer (HGT) is relatively common in plant mitochondrial genomes but the mechanisms, extent and consequences of remain largely unknown. Previous results indicate that parasitic plants are often involved as either donors or recipients, suggesting direct contact between parasite host facilitates genetic among plants. In order to uncover mechanistic details plant-to-plant HGT, evolutionary fate was investigated two groups: genus Cuscuta a small clade Plantago species. A broad...
Article8 July 2019Open Access Source DataTransparent process Mitochondrial MsrB2 serves as a switch and transducer for mitophagy Seung Hee Lee Corresponding Author [email protected] orcid.org/0000-0003-3642-1416 Yale Cardiovascular Research Center, Section of Medicine, Department Internal University School New Haven, CT, USA Division Diseases, Center Biomedical Sciences, National Institute Health, Cheongju, Chungbuk, Korea Search more papers by this author Suho Departments Neurology...
Aging is a complex physiological phenomenon, intricately associated with cardiovascular pathologies, where platelets play central pathophysiological role. Although antiplatelets are commonly employed to prevent and treat major adverse events, aging intraplatelet changes remain largely unexplored.Platelets were studied in high risk patients (aged 40-100 years) comparing them younger healthy subjects. This was followed by cross sectional longitudinal mice studies. Flow cytometry, biochemical...
To study the diversity of immune receptors and pathogens, multiplex PCR has become a central approach in research diagnostics. However, insufficient primer design against highly diverse templates often prevents amplification therefore limits correct understanding biological processes. Here, we present openPrimeR, an R-based tool for evaluating designing primers. openPrimeR provides functional intuitive interface uses either greedy algorithm or integer linear program to compute minimal set...
Current understanding of tumor immunosuppressive mechanisms forms the basis for modern day immunotherapies. Immunoregulatory role platelets in cancer remains largely elusive. Platelets from non-small cell lung (NSCLC) patients revealed a distinct activation phenotype. TREM-like transcript 1 (TLT-1), platelet protein, was increased along with enhanced extracellular release NSCLC platelets. The TLT-1 also evident humanized mice patient-derived tumors. In immunocompetent syngeneic tumors,...
Background: Unfolded protein response (UPR) is a multifaceted signaling cascade that alleviates misfolding. Although well studied in nucleated cells, UPR absence of transcriptional regulation has not been described. Intricately associated with cardiovascular diseases, platelets, despite being anucleate, respond rapidly to stressors blood. We investigate the anucleate platelets and explore its role, if any, on platelet physiology function. Methods: Human mouse were using combination ex vivo...
Extensive research in pedestrian dynamics has primarily focused on crowded conditions and associated phenomena, such as lane formation, evacuation, etc. Several force-based models have been developed to predict the behavior these situations. In contrast, there is a notable gap terms of investigations moderate-to-low density These scenarios are extremely commonplace across world, including highly populated nations like India. Additionally, details expected show significant effects at...
In the existing literature, pedestrian dynamics models have successfully captured various complex scenarios such as lane formation, evacuation, bottlenecks, crowd intersections, etc. However, many models, particularly force-based struggle to replicate simple yet real-life due absence of human intelligence during processes. this study, we present a novel Variable Goal Approach (VGA), which incorporates into significantly enhancing their efficiency and realism. VGA introduces concept multiple...
Purpose: This study compares clinical characteristics of retinitis pigmentosa (RP) associated with mutations in the EYS and USH2A genes a Southeast Asian cohort. Methods: Prospective single-center families EYS- or USH2A-associated RP seen at Singapore National Eye Centre. Comprehensive ophthalmic evaluations, multimodal imaging, genetic testing, longitudinal follow-up identified clinically useful differentiating features between two genotypes. Results: A total 300 were enrolled, RP,...
Purpose To assess the utility of fundus autofluorescence (FAF) patterns for predicting EYS genotype in retinitis pigmentosa (RP) patients. Methods This retrospective, multi-institutional study analyzed FAF images from 200 RP patients (74 with and 126 without ) Singapore Japan. Seven including infinity sign a broad banded hyper-autofluorescent leading edge were evaluated their association genotype. Results The hyperautofluorescent occurred more frequently eyes (p = 0.0014 p 0.036...
To analyze the phenotypic and genotypic characteristics of inherited retinal degeneration (IRD) patients Malay Indian ethnicity from Singapore Malaysia. Ethnic IRD were consecutively enrolled retina clinics in Phenotypic genetic data reviewed. A total 100 unrelated individuals (Malay: n = 46, Indian: 54) enrolled. Sixteen distinct phenotypes identified, with nonsyndromic retinitis pigmentosa (RP) comprising 46% all cases. Stargardt disease cone-rod dystrophy accounted for 20% 11% cases,...
Abstract As the discovery of new genes causing inherited retinal disease (IRD) has plateaued, we look to other factors which could be used maximize diagnostic yield. We analyzed whole-exome sequencing (WES) data from 506 IRD probands, focusing on interplay between yield, age symptom onset or diagnosis, family history, and initial clinical diagnosis. The cohort’s overall yield was 49.2%. Diagnostic negatively correlated with positively number affected members. Diseases distinctive...
In today’s business world, large-scale MSBI and BusinessObjects deployments are essential to fuel data-driven decision-making. Strategic modernization proper management of the systems key sustaining competitive edge delivering strong analytics capabilities. This paper discusses best practices in managing sophisticated BI architecture with focus on an active approach combining risk assessment, performance tuning, data governance. It dangers system upgrades—e.g., minimizing disruption...
Jain, Kanika, Geetha Suryakumar, Rajendra Prasad, and Lilly Ganju. Upregulation of cytoprotective defense mechanisms hypoxia-responsive proteins imparts tolerance to acute hypobaric hypoxia. High Alt Med Biol 14:65–77, 2013.—Exposure high altitude is a well-known environmental stress with physiological metabolic consequences, the major stressor being The disruption in cellular homeostasis elicits several chronic adaptations designed diminish imposed by hypoxic insult. Highly conserved...