A5012451683- Metabolism, Diabetes, and Cancer
- Fungal and yeast genetics research
- Nitric Oxide and Endothelin Effects
- Microbial Metabolic Engineering and Bioproduction
- Pancreatic function and diabetes
- Natural Antidiabetic Agents Studies
- Endoplasmic Reticulum Stress and Disease
- Cardiovascular Issues in Pregnancy
- Renin-Angiotensin System Studies
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Sirtuins and Resveratrol in Medicine
- PI3K/AKT/mTOR signaling in cancer
- Genetic factors in colorectal cancer
- Vitamin D Research Studies
- Eicosanoids and Hypertension Pharmacology
- Plant nutrient uptake and metabolism
- Enzyme-mediated dye degradation
- Neonatal and fetal brain pathology
- Neonatal Health and Biochemistry
- Fungal Biology and Applications
- Glycogen Storage Diseases and Myoclonus
- Protein Kinase Regulation and GTPase Signaling
- Biochemical effects in animals
University of Glasgow
2020-2024
Imperial College London
2015
Hammersmith Hospital
2002-2011
University of Minnesota
2007
Twin Cities Orthopedics
2007
Columbia University
2003
The Snf1/AMP-activated protein kinase (AMPK) family plays fundamental roles in cellular responses to metabolic stress eukaryotes. In humans, AMPK regulates lipid and glucose metabolism has been implicated such disorders as diabetes obesity cardiac abnormalities. Snf1 are the downstream components of cascades, but upstream kinase(s) have remained elusive. We here identified three yeast kinases, Pak1p, Tos3p, Elm1p, that activate vivo . Triple deletion cognate genes causes a Snf – mutant...
The SNF1 protein kinase complex plays an essential role in regulating gene expression response to the level of extracellular glucose budding yeast. shares structural and functional similarities with mammalian AMP-activated kinase. Both kinases are activated by phosphorylation on a threonine residue within activation loop segment catalytic subunit. Here we show that ADP is long-sought metabolite activates limitation protecting enzyme against dephosphorylation Glc7, its physiologically...
Perinatal hypoxic-ischaemic encephalopathy (HIE) occurs in 1-2 every 1000 term infants and the devastating consequences range from cerebral palsy, epilepsy neurological deficit to death. Cellular damage post insult after a delay is mediated by secondary neural energy failure. AMP-activated protein kinase (AMPK) sensor of cellular stress resulting ATP depletion and/or calcium dysregulation, hallmarks neuronal cell death observed HIE. AMPK activation has been implicated models adult ischaemic...
Increased vasoreactivity due to reduced endothelial NO bioavailability is an underlying feature of cardiovascular disease, including hypertension. In small resistance arteries, declining enhances vascular smooth muscle (VSM) reactivity partly by enabling rapid depolarizing Ca
Abstract Objective —To evaluate whether biochemical or genetic alterations in AMP-activated protein kinase (AMPK) play a role the development of polysaccharide storage myopathy (PSSM) Quarter Horses. Animals —30 PSSM-affected and 30 unaffected (control) Procedures —By use an established peptide phosphotransfer assay, basal maximal AMPK activities were measured muscle biopsy samples obtained from 6 control horses. In 24 horses, microsatellite markers identified chromosomal locations all 7...
The PKC1 gene in the yeast Saccharomyces cerevisiae encodes for protein kinase C which is known to control a MAP cascade consisting of different kinases: Bck1, Mkk1 and Mkk2, Mpk1. This affects cell wall integrity but phenotype pkc1Δ mutants suggests additional targets that have not yet been identified [Heinisch et al., Mol. Microbiol. 32 (1999) 671–680]. mutant, as opposed other cascade, displays defects carbon metabolism. One them occurs derepression SUC2 after exhaustion glucose from...
The PKC1 gene in the yeast Saccharomyces cerevisiae encodes protein kinase C that is known to control a mitogen-activated (MAP) cascade consisting of Bck1, Mkk1 and Mkk2, Mpk1. This affects cell wall integrity but phenotype Pkc1 mutants suggests additional targets which have not yet been identified. We show pkc1Δ mutant, as opposed MAP cascade, displays two major defects carbon metabolism. It shows delay initiation fermentation upon addition glucose defect derepression SUC2 after exhaustion...
The PKC1 gene in the yeast Saccharomyces cerevisiae encodes protein kinase C that is known to control a mitogen-activated (MAP) cascade consisting of Bck1, Mkk1 and Mkk2, Mpk1. This affects cell wall integrity but phenotype Pkc1 mutants suggests additional targets which have not yet been identified. We show pkc1Δ mutant, as opposed MAP cascade, displays two major defects carbon metabolism. It shows delay initiation fermentation upon addition glucose defect derepression SUC2 after exhaustion...