A5022785507- Gene Regulatory Network Analysis
- RNA Interference and Gene Delivery
- Evolution and Genetic Dynamics
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Genetics, Aging, and Longevity in Model Organisms
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Virus-based gene therapy research
- RNA Research and Splicing
- Ecosystem dynamics and resilience
- Machine Learning in Bioinformatics
- Cancer-related gene regulation
- Animal Virus Infections Studies
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Fibroblast Growth Factor Research
- Health Systems, Economic Evaluations, Quality of Life
- Global Maternal and Child Health
- Advanced Thermodynamics and Statistical Mechanics
- Structural Engineering and Vibration Analysis
- Nanoparticle-Based Drug Delivery
- Single-cell and spatial transcriptomics
- Kruppel-like factors research
- Dietary Effects on Health
State Key Laboratory of Natural Medicine
2021-2024
China Pharmaceutical University
2018-2024
Weizmann Institute of Science
2022-2023
Inserm
2015-2022
Université Paris Cité
2015-2022
Center for Research and Interdisciplinarity
2015-2022
Délégation Paris 5
2016-2018
Peking University
2007
State Key Laboratory of Turbulence and Complex Systems
2007
Genetically identical cells in the same stressful condition die at different times. The origin of this stochasticity is unclear; it may arise from initial conditions that affect time demise, or a stochastic damage accumulation mechanism erases and instead amplifies noise to generate lifespans. To address requires measuring dynamics individual over lifespan, but has rarely been achieved. Here, we used microfluidic device measure membrane 635 carbon-starved Escherichia coli high temporal...
Article23 November 2015Open Access Source Data A synthetic growth switch based on controlled expression of RNA polymerase Jérôme Izard Université Grenoble Alpes, Laboratoire Interdisciplinaire de Physique (CNRS UMR 5588), Saint Martin d'Hères, France INRIA, – Rhône-Alpes research center, Ismier, These authors contributed equally to this work Search for more papers by author Cindy DC Gomez Balderas Delphine Ropers Stephan Lacour Xiaohu Song Center Research and Interdisciplinarity, INSERM...
Despite the well-recognized clinical success of therapeutic proteins, especially antibodies, their inability to penetrate cell membrane restricts them secretory extracellular or membrane-associated targets. Developing a direct cytosolic protein delivery system would offer unique opportunities for intracellular target-related proteins. Here, we generated supercharged polypeptide (SCP) with high cellular uptake efficiency, endosomal escape ability, and good biosafety developed an SCP unnatural...
Natural selection is thought to shape the evolution of aging patterns, although how life-history trajectories orchestrate inherently stochastic processes associated with unclear. Tracking clonal growth-arrested Escherichia coli cohorts in an homogeneous environment at single-cell resolution, we demonstrate that Gompertz law exponential mortality characterizes bacterial lifespan distributions. By disentangling rate from age-independent components longevity, find increasing cellular...
Abstract The efficient delivery of biologics into cells provide unique opportunities to modulate intracellular targets not druggable by conventional small molecules. supercharged polypeptide (SCP) has become a novel system due their special advantages, including enhanced efficiency and serum tolerance. However, owing cationic charge non‐specificity characteristics, the in vivo application SCP is limited. Here, an activatable (ASCP) with pH‐sensitive shielding sequence (CSS), protease...
Abstract Background Despite a remarkable success in the computational prediction of genes Bacteria and Archaea, lack comprehensive understanding prokaryotic gene structures prevents from further elucidation differences among genomes. It continues to be interesting develop new ab initio algorithms which not only accurately predict genes, but also facilitate comparative studies Results This paper describes genefinding algorithm based on statistical model protein coding Open Reading Frames...
Correct annotation of translation initiation site (TIS) is essential for both experiments and bioinformatics studies prokaryotic mechanism as well understanding gene regulation structure. Here we describe a comprehensive database ProTISA, which collects TIS confirmed through variety available evidences genomes, including Swiss-Prot record, literature, conserved domain hits sequence alignment between orthologous genes. Moreover, by combining the predictions from our recently developed...
Because of the absence effective therapies for metabolic dysfunction-associated steatohepatitis (MASH), there is a rising interest in fibroblast growth factor 21 (FGF21) analogues due to their potential anti-fibrotic activities MASH treatment. PsTag-FGF21, long-acting FGF21 analogue, has demonstrated promising therapeutic effects several mouse models. However, its efficacy and mechanism against MASH-related fibrosis remain less well defined, compared with specific mechanisms through which...
Abstract Natural selection has long been hypothesised to shape ageing patterns, but whether and how contributes life-history evolution remains elusive. The complexity of various ageing-associated molecular mechanisms their inherent stochasticity hinder reductionist approaches the understanding functional senescence, i.e . reduced fecundity increased mortality. Recent bio-demographic work demonstrated that high-precision statistics traits such as mortality rates could be used...
Genetically identical cells in the same stressful condition die at different times. The origin of this stochasticity is unclear; it may arise from initial conditions that affect time demise, or a stochastic damage accumulation mechanism erases and instead amplifies noise to generate lifespans. To address requires measuring dynamics individual over lifespan, but has rarely been achieved. Here, we used microfluidic device measure membrane 648 carbon-starved E. coli high temporal resolution. We...
To evaluate the accessibility of essential medicines in China through a new system which integrated structure, process, and effect National Essential Medicine Policy (NEMP).A structural equation model was built to verify reliability evaluation system. This study utilized Delphi method obtain structure process index data, used WHO/HAI standard for data NEMP. Six regions were selected empirical analysis so that suggestions optimization could be put forward.The consisted three parts:...
Abstract Longevity research aims to enhance the period of healthy life, known as healthspan, while minimizing duration disability and morbidity, sickspan. Various interventions extend lifespan in model organisms, but it is not which these are likely compress relative Here, we present a theory that predicts effectively The based on shape survival curve - fraction organisms surviving function age. Interventions lengthen mean preserving curve, scaling, hypothesized sickspan proportionally,...