A5031122180- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Chronic Lymphocytic Leukemia Research
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Chronic Myeloid Leukemia Treatments
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Eosinophilic Esophagitis
- Cancer-related molecular mechanisms research
- IL-33, ST2, and ILC Pathways
MRC London Institute of Medical Sciences
2022-2023
Institute of Clinical Research
2023
Imperial College London
2022
University of Cambridge
2019-2020
Early single-cell RNA-seq (scRNA-seq) studies suggested that it was unusual to see more than one isoform being produced from a gene in single cell, even when multiple isoforms were detected matched bulk samples. However, these generally did not consider the impact of dropouts or quantification errors, potentially confounding results analyses.
Abstract Gene enhancers often form long-range contacts with promoters, but it remains unclear if the activity of and their chromosomal are mediated by same DNA sequences recruited factors. Here, we study effects expression quantitative trait loci (eQTLs) on enhancer promoter in primary monocytes isolated from 34 male individuals. Using eQTL-Capture Hi-C a Bayesian approach considering both intra- inter-individual variation, initially detect 19 eQTLs associated enhancer-eGene contacts, most...
JW was supported by a BBSRC DTP studentship BB/M011194/1. MH funded core grant from the Wellcome Trust. AFS the Medical Research Council, MR/R009791/1.
Abstract Gene enhancers often form long-range contacts with promoters, but it remains unclear if enhancer activity and their chromosomal are mediated by the same DNA sequences recruited factors. We studied effects of expression quantitative trait loci (eQTLs) on promoter in primary monocytes isolated from 34 individuals. Using eQTL-Capture Hi-C a Bayesian approach considering both intra- inter-individual variation, we initially detected 19 eQTLs associated enhancer-eGene contacts, most which...
Abstract Innate lymphoid cells (ILCs) are rare tissue-resident “helper” lymphocytes that do not express diversified antigen receptors. Type 3 ILCs (ILC3s) an important class of these enriched in the respiratory and intestinal mucosa, where they regulate inflammation mucosal homeostasis. To gain insight into cis-regulatory circuitries underlying ILC3 function, we used high-resolution Capture Hi-C to profile promoter-anchored chromosomal contacts human primary ILC3s. Combining significant...
Abstract Background Early single-cell RNA-seq (scRNA-seq) studies suggested that it was unusual to see more than one isoform being produced from a gene in single cell, even when multiple isoforms were detected matched bulk samples. However, these generally did not consider the impact of dropouts or quantification errors, potentially confounding results analyses. Results In this study, we take simulation based approach which explicitly account for and errors. We use our simulations ask what...
Early single-cell RNA-seq (scRNA-seq) studies suggested that it was unusual to see more than one isoform being produced from a gene in single cell, even when multiple isoforms were detected matched bulk samples. However, these generally did not consider the impact of dropouts or quantification errors, potentially confounding results analyses. In this study, we take simulation based approach which explicitly account for and errors. We use our simulations ask what extent is possible study...