A5002221728- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Bacteriophages and microbial interactions
- Force Microscopy Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- Pluripotent Stem Cells Research
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Phagocytosis and Immune Regulation
Cornell University
2024
Weill Cornell Medicine
2024
Korea Advanced Institute of Science and Technology
2022-2023
Abstract Neurogenin 3 (NGN3) is a key transcription factor in the cell fate determination of endocrine progenitors (EPs) developing pancreas. Previous studies have shown that stability and activity NGN3 are regulated by phosphorylation. However, role methylation poorly understood. Here, we report protein arginine methyltransferase-1 (PRMT1)-mediated 65 required for pancreatic development human embryonic stem cells (hESCs) vitro. We found inducible PRMT1-knockout (P-iKO) hESCs did not...
Changes in the extracellular matrix (ECM) influence stem cell fate. When hESCs were differentiated on a thin layer of Matrigel coated onto PDMS (Matrigel_PDMS), they exhibited substantial increase focal adhesion and adhesion-associated proteins compared with those cultured TCPS (Matrigel_TCPS), resulting YAP/TEF1 activation ultimately promoting transcriptional activities pancreatic endoderm (PE)-associated genes. Interestingly, YAP PE cells was mediated through integrin α3-FAK-CDC42-PP1A...
Abstract In the developing pancreas, Neurogenin 3 (NGN3) is a key transcription factor in cell fate determination of endocrine progenitors (EPs). Although activation and stability NGN3 are regulated by phosphorylation, role arginine methylation poorly understood. Here, we report 65 absolutely required for pancreatic lineage development human embryonic stem cells (hESCs) vitro . First, found inducible protein methyltransferase 1 (PRMT1)-knockout (P-iKO) hESCs did not differentiate from EPs to...