A5011923694- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Autophagy in Disease and Therapy
- Organ Transplantation Techniques and Outcomes
- Liver physiology and pathology
- Pluripotent Stem Cells Research
- Electromagnetic Fields and Biological Effects
- 3D Printing in Biomedical Research
- Spinal Cord Injury Research
- Cancer Cells and Metastasis
- Ocular Surface and Contact Lens
- Bone health and osteoporosis research
- Advanced Glycation End Products research
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Nerve injury and regeneration
- Mitochondrial Function and Pathology
- Corneal Surgery and Treatments
- Advanced biosensing and bioanalysis techniques
- Magnetic and Electromagnetic Effects
- Cancer, Hypoxia, and Metabolism
- Electrospun Nanofibers in Biomedical Applications
- Bone fractures and treatments
- Spaceflight effects on biology
- Telomeres, Telomerase, and Senescence
Tzu Chi Foundation
2021
Buddhist Tzu Chi General Hospital
2013-2019
Hualien Tzu Chi Medical Center
2019
National Yang Ming Chiao Tung University
2009-2011
National Dong Hwa University
2009-2010
Abstract Pulsed electromagnetic fields (PEMFs) have been used clinically to slow down osteoporosis and accelerate the healing of bone fractures for many years. The aim this study is investigate effect PEMFs on proliferation differentiation potential human marrow mesenchymal stem cells (BMMSC). PEMF stimulus was administered BMMSCs 8 h per day during culture period. applied consisted 4.5 ms bursts repeating at 15 Hz, each burst contained 20 pulses. Results showed that about 59% 40% more...
Abstract Human mesenchymal stem cells (hMSCs) contribute to ischemic tissue repair, regeneration and possess ability self-renew. However, poor viability of transplanted hMSCs within tissues has limited its therapeutic efficiency. Therefore, it is urgent explore new method improve the grafted cells. By using a systematic analysis, we reveal mechanism synergistic protection N-acetylcysteine (NAC) ascorbic acid 2-phosphate (AAP) on that were under H 2 O -induced oxidative stress. The combined...
Osteogenesis is a complex series of events involving the differentiation mesenchymal stem cells to generate new bone. In this study, we examined effect pulsed electromagnetic fields (PEMFs) on cell proliferation, alkaline phosphatase (ALP) activity, mineralization extracellular matrix, and gene expression in bone marrow (BMMSCs) during osteogenic differentiation. Exposure BMMSCs PEMFs increased proliferation by 29.6% compared untreated at day 1 Semi-quantitative RT-PCR indicated that...
For reasons of provision highly-specific surface area and three-dimensional culture, microcarrier culture (MC) has garnered great interest for its potential to expand anchorage-dependent stem cells. This study utilizes MC in vitro expansion human bone marrow mesenchymal cells (BMMSCs) analyses effects on BMMSC proliferation differentiation.Effects semi-continuous compared control plate (PC) serial bead-to-bead transfer (MC bead-T) BMMSCs were investigated. Cell population growth kinetics,...
Abstract Background Antioxidants have been shown to enhance the proliferation of adipose-derived mesenchymal stem cells (ADMSCs) in vitro , although detailed mechanism(s) and potential side effects are not fully understood. In this study, human ADMSCs cultured ImF-A medium supplemented with antioxidants ( N -acetyl- l -cysteine ascorbic acid-2-phosphate) fibroblast growth factor 2 (FGF-2) were compared FGF-2 alone (ImF) or under 5% pO conditions (ImF-H). Results During log-phase growth,...
Excessive reactive oxygen species is the major component of a harsh microenvironment after ischemia/reperfusion injury in human tissues. Combined treatment N-acetyl-L-cysteine (NAC) and L-ascorbic acid 2-phosphate (AAP) promoted growth mesenchymal stem cells (hMSCs) suppressed oxidative stress-induced cell death by enhancing mitochondrial integrity function vitro. In this study, we aimed to determine whether NAC AAP (termed MCA) could enhance therapeutic potential hMSCs. We established...
There is currently no effective treatment method available for liver fibrosis. We therefore evaluated the use of Wharton's jelly stem cells (WJSCs; major umbilical cord cell population) to treat chemically induced fibrosis via intraperitoneal injection thioacetamide. WJSCs were transplanted into liver-damaged rats portal vein and was by assessing serum biochemistry histopathology. Transplanted distributed in fibrotic area around blood vessels, hepatic recovery accelerated. Serum prothrombin...
Bone marrow-derived mesenchymal cells (BM-MSCs) are able to differentiate into adipocytes, which can secrete adipokines affect BM-MSC proliferation and differentiation. Recent evidences indicated that adipocytes fatty acid metabolites, such as palmitic methyl ester (PAME), is cause vasorelaxation exerts anti-inflammatory effects. However, effects of PAME on remain unclear. The aim this study was investigate the effect human (hBM-MSC) its underlying molecular mechanisms. hBM-MSCs were treated...
Aim: The therapeutic effects of human wisdom teeth-derived neuronal stem cell (tNSC) cotreatment with granulocyte-colony-stimulating factor (G-CSF) were evaluated for contusion-induced spinal cord injury in rats. Materials & methods: 7 days after contusion, tNSCs transplanted to the site and followed by G-CSF 5 days. Behavioral deficits Basso, Beattie Bresnahan test. was collected immunohistochemistry analysis. Results: test significantly improved cotreated group compared or single treatment...
Abstract The present study demonstrated the protective effects of low-molecular-weight adipose-derived stem cell-conditioned medium (LADSC-CM) in a mouse model dry eye syndrome. Mice subjected to desiccating stress and benzalkonium chloride had decreased tear secretion, impaired corneal epithelial tight junction with microvilli, conjunctival goblet cells. Topical application (ADSC-CM) stimulated lacrimal preserved microvilli epithelium, increased density cells MUC16 expression conjunctiva....