A5087779277- Neuroscience and Neuropharmacology Research
- Circadian rhythm and melatonin
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Genetic Neurodegenerative Diseases
- MicroRNA in disease regulation
- Light effects on plants
- Neurogenesis and neuroplasticity mechanisms
- Ion channel regulation and function
- Genetics, Aging, and Longevity in Model Organisms
- Sleep and Wakefulness Research
- Neurological disorders and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Circular RNAs in diseases
- Spaceflight effects on biology
- Melanoma and MAPK Pathways
- RNA Research and Splicing
- Signaling Pathways in Disease
- Birth, Development, and Health
- Axon Guidance and Neuronal Signaling
- Receptor Mechanisms and Signaling
- Cancer Treatment and Pharmacology
- Genetics and Neurodevelopmental Disorders
- Retinal Development and Disorders
- Neuroscience and Neural Engineering
The Ohio State University
2013-2024
Ohio Supercomputer Center
2017
University of Alabama at Birmingham
2012
Yale University
2010
University of Pittsburgh
1992-2000
Abstract Formation of reactive oxygen and nitrogen species is a precipitating event in an array neuropathological conditions. In response to excessive (ROS) levels, transcriptionally dependent mechanisms drive the up‐regulation ROS scavenging proteins which, turn, limit extent brain damage. Here, we employed transgenic approach which cAMP‐response element binding protein (CREB)‐mediated transcription repressed (via A‐CREB) examine contribution CREB/cAMP pathway neuroprotection its potential...
Adult progenitor cell proliferation in the subgranular zone (SGZ) of dentate gyrus is a dynamic process that modulated by an array physiological process, including locomotor activity and novel environmental stimuli. In addition, pathophysiological events, such as ischemia status epilepticus (SE), have been shown to stimulate neurogenesis. Currently, limited information available regarding extracellular stimuli, receptors, downstream intracellular effectors couple excitotoxic stimulation...
A large body of literature has shown that the disruption circadian clock timing profound effects on mood, memory and complex thinking. Central to this time keeping process is master pacemaker located within suprachiasmatic nucleus (SCN). Of note, central nervous system, not exclusive SCN, but rather, ancillary oscillatory capacity been detected in a wide range cell types brain regions, including forebrain circuits underlie cognitive processes. These observations raise questions about...
The dual nature of the NMDA receptor as a mediator excitotoxic cell death and activity-dependent survival likely results from divergent patterns kinase activation, transcription factor gene expression. To begin to address this divergence, we examined cellular molecular signaling events that couple nontoxic levels stimulation activation cAMP response element-binding protein (CREB)/cAMP element (CRE) pathway in cultured cortical neurons. Pulses (10 min) receptor-mediated synaptic activity...
Activation of ionotropic glutamate receptors causes increases in intracellular Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) and Na<sup>+</sup>concentration neurons. It has been suggested that reversal the plasma membrane Na<sup>+</sup>/Ca<sup>2+</sup> exchanger (NCE) may account part for rise [Ca<sup>2+</sup>]<sub>i</sub>. Recently, KB-R7943 (2-[2-[4-(4-nitrobenzyloxy)phenyl]ethyl]isothiourea methanesulfonate) was reported to selectively inhibit reverse mode NCE non-neuronal...
Abstract Signalling via the p42/44 mitogen‐activated protein kinase (MAPK) pathway has been identified as an intermediate event coupling light to entrainment of mammalian circadian clock located in suprachiasmatic nucleus (SCN). Given this observation, it was interest determine where within process MAPK functioning. In study, we examined role a regulator light‐induced gene expression SCN. Towards end, characterized effect pharmacological disruption cascade on immediate‐early genes c‐Fos,...
Disruption of cAMP response element (CRE)-dependent transcription has been hypothesized to contribute neuronal death and dysfunction in Huntington's disease (HD) other polyglutamine repeat disorders. Whether dysregulation CRE-dependent actually occurs vivo expression expanded repeats not tested. We directly tested whether is affected by cross breeding a transgenic mouse model HD (line R6/2) with that expresses CRE-regulated reporter gene. Instead compromised mice, we found robust...
The CREB/CRE transcriptional pathway has been implicated in circadian clock timing and light-evoked resetting. To date, much of the work on CREB physiology focused how changes phosphorylation state regulate processes. However, beyond phosphorylation, CREB-dependent transcription can also be regulated by coactivator CRTC (CREB-regulated coactivator), known as TORC (transducer CREB). Here we profiled both rhythmic regulation CRTC1 CRTC2 murine suprachiasmatic nucleus (SCN), locus master...
The microRNA (miRNA) class of small (typically 22-24 nt) non-coding RNA affects a wide range physiological processes in the mammalian central nervous system (CNS). By acting as potent regulators mRNA translation and stability, miRNAs fine-tune expression multitude genes that play critical roles complex cognitive processes, including learning memory. Of note, within CNS, can be expressed an inducible, cell-type specific manner. Here, we provide brief overview functional effects miR-132/212...
The microRNA miR-132 serves as a key regulator of wide range plasticity-associated processes in the central nervous system. Interestingly, expression has also been shown to be under control circadian timing This finding, coupled with work showing that is expressed hippocampus, where it influences neuronal morphology and memory, led us test idea daily rhythms within forebrain modulate cognition function time. Here, we show hippocampal gated by time-of-day, peak levels occurring during night....
Abstract: Increasing extracellular pH from 7.4 to 8.5 caused a dramatic increase in the time required recover glutamate (3 µ M , for 15 s)‐induced intracellular Ca 2+ concentration ([Ca ] i ) indo‐1‐loaded cultured cortical neurons. Recovery HEPES‐buffered saline solution (HBSS) was 126 ± 30 s, whereas recovery 216 19 s when increased 8.5. Removal of did not inhibit prolongation by increasing pH. Extracellular alkalinization rapid following exposure, suggesting that HBSS may delay affecting...
Within the suprachiasmatic nucleus (SCN)—the locus of master circadian clock— transcriptional regulation via CREB/CRE pathway is implicated in functioning molecular clock timing process, and a key conduit through which photic input entrains oscillator. One event driving CRE-mediated transcription phosphorylation CREB at serine 133 (Ser ). Indeed, numerous reporter gene assays have shown that an alanine point mutation Ser reduces CREB-mediated transcription. Here, we sought to examine...
Glutamate receptor activation has been previously shown to result in mitochondrial depolarization and of the permeability transition pore cultured neurones. In this study, we characterized effects two putative inhibitors, namely trifluoperazine dibucaine, on rat intact, forebrain Permeability was monitored by following neurones loaded with membrane potential‐sensitive fluorescent indicator, JC‐1. Trifluoperazine (10–20 μ M ) dibucaine (50–100 inhibited or delayed onset glutamate‐induced...