A5001589669- Neuroscience of respiration and sleep
- Neonatal Respiratory Health Research
- Extracellular vesicles in disease
- Neurogenesis and neuroplasticity mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- MicroRNA in disease regulation
- Light effects on plants
- Anesthesia and Neurotoxicity Research
- Genetics and Neurodevelopmental Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- 3D Printing in Biomedical Research
- Axon Guidance and Neuronal Signaling
- Neuroscience and Neural Engineering
- Sleep and Wakefulness Research
- Congenital Diaphragmatic Hernia Studies
- Nanoplatforms for cancer theranostics
- Genetic Neurodegenerative Diseases
- Retinal Development and Disorders
- Skin Protection and Aging
- Mesenchymal stem cell research
- Immune cells in cancer
- Functional Brain Connectivity Studies
- Microfluidic and Bio-sensing Technologies
- Melanoma and MAPK Pathways
The Ohio State University
2014-2025
Zero to Three
2020
Fibroblasts nonvirally programmed to convert into induced endothelial cells show therapeutic potential for ischemic stroke.
The CREB/CRE transcriptional pathway has been implicated in circadian clock timing and light-evoked resetting. To date, much of the work on CREB physiology focused how changes phosphorylation state regulate processes. However, beyond phosphorylation, CREB-dependent transcription can also be regulated by coactivator CRTC (CREB-regulated coactivator), known as TORC (transducer CREB). Here we profiled both rhythmic regulation CRTC1 CRTC2 murine suprachiasmatic nucleus (SCN), locus master...
The microRNA miR-132 serves as a key regulator of wide range plasticity-associated processes in the central nervous system. Interestingly, expression has also been shown to be under control circadian timing This finding, coupled with work showing that is expressed hippocampus, where it influences neuronal morphology and memory, led us test idea daily rhythms within forebrain modulate cognition function time. Here, we show hippocampal gated by time-of-day, peak levels occurring during night....
Abstract Gene/oligonucleotide therapies have emerged as a promising strategy for the treatment of different neurological conditions. However, current methodologies delivery neurogenic/neurotrophic cargo to brain and nerve tissue are fraught with caveats, including reliance on viral vectors, potential toxicity, immune/inflammatory responses. Moreover, central nervous system is further compounded by low permeability blood barrier. Extracellular vesicles (EVs) vehicles therapies, overcoming...
Tumor-associated immune cells play a crucial role in cancer progression. Myeloid-derived suppressor (MDSCs), for example, are immature innate that infiltrate the tumor to exert immunosuppressive activity and protect from host's system and/or cancer-specific immunotherapies. While tumor-associated have emerged as promising therapeutic target, efforts counter immunosuppression within niche been hampered by lack of approaches selectively target cell compartment tumor, effectively eliminate...
Abstract The neurogenic niche within the subgranular zone ( SGZ ) of dentate gyrus is a source new neurons throughout life. Interestingly, proliferative capacity regulated by both physiological and pathophysiological conditions. One outstanding question involves molecular mechanisms that regulate basal inducible adult neurogenesis. Here, we examined role MAPK ‐regulated kinases, mitogen‐ stress‐activated kinase MSK )1 2. as regulators progenitor cell proliferation Under conditions, 1/2 null...
Congenital central hypoventilation syndrome (CCHS) represents a rare genetic disorder usually caused by mutations in the homeodomain transcription factor PHOX2B. Some CCHS patients suffer mainly from deficiencies CO2 and/or O2 respiratory chemoreflex, whereas other present with full apnea shortly after birth. Our goal was to identify neuropathological mechanisms of apneic presentations CCHS. In developing murine neuroepithelium, Phox2b is expressed three discrete progenitor domains across...
Mitogen-activated protein kinase (MAPK) signaling has been implicated in a wide range of neuronal processes, including development, plasticity, and viability. One the principal downstream targets both extracellular signal-regulated kinase/MAPK pathway p38 MAPK is Mitogen- Stress-activated Kinase 1 (MSK1). Here, we sought to understand role that MSK1 plays neuroprotection against excitotoxic stimulation hippocampus. To this end, utilized immunohistochemical labeling, null mouse line, cell...
The mammalian master circadian pacemaker within the suprachiasmatic nucleus (SCN) maintains tight entrainment to 24 hr light/dark cycle via a sophisticated clock-gated rhythm in responsiveness of oscillator light. A central event this light process appears be rapid induction gene expression ERK/MAPK pathway. Here, we used RNA array-based profiling combination with pharmacological disruption methods examine contribution signaling light-evoked expression. Transient photic stimulation during...
As the healthcare burden caused by an increasingly aging population rapidly rises, a pressing need exists for innovative geroscience research that can elucidate mechanisms and precipitate development of therapeutic interventions to support healthy aging. The Fifth Annual Midwest Aging Consortium Research symposium, held from April 28-30, 2024, was hosted Ohio State University in Columbus, featured presentations investigators across Midwestern United States. This report summarizes presented...
Hindbrain respiratory neuron networks undergo critical developmental maturation during the late embryonic, perinatal and post‐natal periods in mammals. Thus, abnormalities network development may contribute to autonomic dysfunction. Central Congenital Hypoventilation Syndrome (CCHS) is a rare genetic disorder characterized by an inability sense respond altered levels of CO 2 or O (Chemosensation). CCHS patients present with spectrum central control pathophysiologies, ranging from deficiency...
The transcription factor Phox2b is a regulator of the autonomic nervous system development and especially expressed by respiratory circuits involved in chemosensory integration, as neurons solitary tract nucleus (NTS) that relay information from peripheral chemoreceptors to ventral column, central located retrotrapezoid (RTN). In addition neurons, recent studies show astrocytes medullary surface also detect PCO 2 increase, contribute drive. Since PHOX2B biased progenitor cells generate have...
Current methods to analyze respiratory behavior utilize plethysmography acquire standing values of frequency, volume and flow at specific time points in murine maturation. However, these factors have been independently analyzed as a function with no further analysis the interplay variables amongst each other context We developed R‐based analytical workflow examine how variation relationships frequency (f) tidal (TV) respect inspiratory expiratory parameters shift age mice. Our evidences that...
Functionalized Extracellular Vesicles In article number 2100805 by Natalia Higuita-Castro and co-workers, designer extracellular vesicles (EVs) are manufactured via non-viral engineering of donor cells through electroporation. Designer EVs packed with plasmid DNA, mRNA, protein content. Transfecting expression plasmids for membrane proteins can be used to tailor the surface decoration EVs. Pro-neurogenic drive neurogenesis improve targeting neuronal tissue, in vitro vivo.