A5021110994- DNA and Nucleic Acid Chemistry
- Cardiomyopathy and Myosin Studies
- Force Microscopy Techniques and Applications
- Micro and Nano Robotics
- Advanced biosensing and bioanalysis techniques
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Molecular Junctions and Nanostructures
- Cancer therapeutics and mechanisms
- Advanced Materials and Mechanics
- Modular Robots and Swarm Intelligence
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Advanced Thermodynamics and Statistical Mechanics
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Pickering emulsions and particle stabilization
- Nanopore and Nanochannel Transport Studies
- Developmental Biology and Gene Regulation
- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Cellular transport and secretion
- Fuel Cells and Related Materials
- Photoreceptor and optogenetics research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Stanford University
2016-2025
Stanford Medicine
2009-2025
Chan Zuckerberg Initiative (United States)
2025
Bioengineering Center
2017-2023
Institute of Structural and Molecular Biology
2013
University of California, Berkeley
2000-2007
Lawrence Berkeley National Laboratory
2002-2007
Korea University
2007
Harvard University
2007
Massachusetts Institute of Technology
2007
Water-soluble, highly fluorescent, silanized semiconductor nanocrystals with different surface charges were synthesized. To covalently attach the to biological macromolecules a variety of mild coupling chemistries, outermost siloxane shells derivatized thiol, amino, or carboxyl functional groups. Single- double-stranded DNA was coupled nanocrystal surfaces by using commercially available bifunctional cross-linker. Conjugation had little effect on optical properties nanocrystals, and...
Escherichia coli topoisomerase (Topo) IV is an essential type II Topo that removes DNA entanglements created during replication. relaxes (+) supercoils much faster than (–) supercoils, promoting replication while sparing the supercoils. Here, we investigate mechanism underlying this chiral preference. Using binding assays and a single-molecule braiding system, show recognizes crossings imposed by left-handed superhelix of supercoiled DNA, rather global topology, twist deformation, or local...
The CRISPR-Cas9 nuclease has been widely repurposed as a molecular and cell biology tool for its ability to programmably target cleave DNA. Cas9 recognizes site by unwinding the DNA double helix hybridizing 20-nucleotide section of associated guide RNA one strand, forming an R-loop structure. A dynamic mechanical description formation is needed understand biophysics searching develop rational approaches mitigating off-target activity while accounting influence torsional strain in genome....
Significance Artificial intelligence holds considerable promise for transforming quantitative modeling in materials science. We illustrate this potential by developing machine-learning models of a paradigmatic class biomaterials called active nematics. These hybrid can be viewed as artificial muscles composed biological fibers and molecular motors. Here, the macroscopic coefficients characterizing energy injection motors material elasticity are not constant. They unknown functions space time...
B-DNA becomes unstable under superhelical stress and is able to adopt a wide range of alternative conformations including strand-separated DNA Z-DNA. Localized sequence-dependent structural transitions are important for the regulation biological processes such as replication transcription. To directly probe effect sequence on driven by torque, we have measured torsional response panel sequences using single molecule assays that employ nanosphere rotational probes achieve high torque...
Despite extensive scrutiny of the myosin superfamily, lack high-resolution structures actin-bound states has prevented a complete description its mechanochemical cycle and limited insight into how sequence structural diversification motor domain gives rise to specialized functional properties. Here we present cryo-EM unique minus-end directed VI in rigor (4.6 Å) Mg-ADP (5.5 bound F-actin. Comparison IIC-F-actin complex reveals an almost conservation residues at actin-myosin interface despite...
In active materials, motor proteins produce activity while also modulating elasticity.
Axis formation in Drosophila depends on correct patterning of the follicular epithelium and signaling between germ line soma during oogenesis. We describe a method for identifying genes expressed follicle cells with potential roles axis formation. Follicle are purified from whole ovaries by enzymatic digestion, filtration, fluorescence-activated cell sorting (FACS). Two strategies used to obtain complementary groups. In first strategy, spatially restricted subpopulations marked FACS...
Abstract The fates of two small subgroups the ovarian follicle cells appear to be linked: mutations in Notch, Delta, fs(1)Yb, or hedgehog cause simultaneous defects specification stalk and polar cells. Both these are determined germarium, both cease division early oogenesis. To test possibility that related by lineage, we generated dominantly marked mitotic clones ovaries. Small, restricted were found adjacent each other at a frequency much too high explained independent induction. We...
Myosin VI supports movement toward the (−) end of actin filaments, despite sharing extensive sequence and structural homology with (+)-end-directed myosins. A class-specific stretch amino acids inserted between converter domain lever arm was proposed to provide basis directionality reversal. Indeed, unique insert mediates a 120° redirection in crystal structure presumed poststroke conformation myosin [Ménétrey J, Bahloul A, Wells AL, Yengo CM, Morris CA, Sweeney HL, Houdusse (2005) Nature...
Multipolarization microscopy shows tight linkage of the rotational dynamics axonal endosomes to molecular motor activity.