A5074774307- Cancer therapeutics and mechanisms
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- Neutropenia and Cancer Infections
- Cancer-related Molecular Pathways
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Cancer, Hypoxia, and Metabolism
- Enzyme Structure and Function
- NF-κB Signaling Pathways
- Bioactive Compounds and Antitumor Agents
- Peptidase Inhibition and Analysis
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Cell death mechanisms and regulation
- ATP Synthase and ATPases Research
- Bioinformatics and Genomic Networks
- Advanced Proteomics Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Fungal and yeast genetics research
Loyola University New Orleans
2021-2024
Genentech
2011-2014
Genetic Analysis (Norway)
2014
Institute of Molecular and Cell Biology
2014
University of California, Berkeley
2005-2012
QB3
2008-2010
Louisiana State University
2003-2004
The University of Tokyo
2001
Inhibitor of apoptosis (IAP) proteins are negative regulators cell death. IAP family members contain RING domains that impart E3 ubiquitin ligase activity. Binding endogenous or small-molecule antagonists to select baculovirus repeat (BIR) within cellular (cIAP) promotes autoubiquitination and proteasomal degradation so releases inhibition mediated by cIAP. Although the molecular details antagonist-BIR domain interactions well understood, it is not clear how this binding event influences...
In contrast to the current state of knowledge in field eukaryotic chromosome segregation, relatively little is known about mechanisms coordinating appropriate segregation bacterial chromosomes. Escherichia coli , MukB/E/F complex and topoisomerase IV (Topo IV) are both crucial players this process. Topo removes DNA entanglements following replication chromosome, whereas MukB, a member structural maintenance chromosomes protein family, serves as condensin. We demonstrate here direct physical...
DNA topos (topoisomerases) are complex, multisubunit enzymes that remodel topology. Members of the type II topo family function by passing one segment duplex through a transient break in another, process consumes two molecules ATP and requires co-ordinated action multiple domains. Recent structural data on ATPase regions, which activate enforce directionality strand passage, have highlighted how physically controls catalytic cycle enzyme. Structural biochemical studies specialized...
DNA topoisomerases manage chromosome supercoiling and organization in all forms of life. Gyrase, a prokaryotic heterotetrameric type IIA topo, introduces negative supercoils into by an ATP-dependent strand passage mechanism. All gyrase orthologs rely on homologous set catalytic domains for function; however, these enzymes also can possess species-specific auxiliary regions. The gyrases many gram-negative bacteria harbor 170-amino acid insertion unknown architecture function the metal-...
Significance The CMV glycoprotein (g) entry complex gH/gL is a significant target of the human immune system, eliciting production highly neutralizing antibodies. MSL-109, monoclonal antibody that binds to gH, potently neutralizes entry. Here, viral mutants resistant MSL-109 were generated and epitope mapped on model structure gH. We have used cell-surface fluorescence assay reveal novel interactions among glycoproteins probe effect interactions. Our data confirm cross-talk between gB,...
The oomycete Hyaloperonospora arabidopsidis (Hpa) is the causal agent of downy mildew on model plant Arabidopsis thaliana and has been adapted as a system to investigate pathogen virulence strategies disease resistance mechanisms. Recognition Hpa infection occurs when proteins (R-genes) detect presence or activity pathogen-derived protein effectors delivered host. This study examines effector ATR13 Emco5 its recognition by RPP13-Nd, cognate R-gene that triggers programmed cell death (HR) in...
Approximately 30% of eukaryotic genomes are predicted to encode partially unfolded proteins. Many these unstructured domains contact multiple partners in short-lived interactions critical for cellular homeostasis. Understanding the functional implications transient binding events is a current challenge that could be addressed with designed peptide inhibitors. Most protein design methodologies, however, target only structurally well-defined, stable structures. To address this limitation, we...
Precise control of supercoiling homeostasis is critical to DNA-dependent processes such as gene expression, replication, and damage response. Topoisomerases are central regulators DNA commonly thought act independently in the recognition modulation chromosome superstructure; however, recent evidence has indicated that cells tightly regulate topoisomerase activity support dynamics, transcriptional response, replicative events. How executed linked internal status a cell poorly understood. To...
Type III CRISPR-Cas systems employ multiprotein effector complexes bound to small CRISPR RNAs (crRNAs) detect foreign RNA transcripts and elicit a complex immune response that leads the destruction of invading DNA. are among most widespread in nature, emerging interest harnessing these for biotechnology applications highlights need detailed structural analyses representatives from diverse organisms. We performed cryo-EM reconstructions III-A Cas10-Csm S. epidermidis an intact, cognate target...
The à 3Πi←X̃ 3Σ− electronic transition of a carbon-chain molecule CCS has been observed for the first time by laser induced fluorescence spectroscopy. species was generated with pulsed discharge 0.5% C2H2 and CS2 diluted in Ar via supersonic jet. A number bands 690–675 nm 600–615 regions (14 500–14 800, 16 200–16 600 cm−1) were assigned to those using ground state combination differences. Three each region found belong three 3Πi spin-orbit components (Tv=14 662.777(4) 423 cm−1), an effective...
Abstract We have examined the chemical denaturations of Klentaq and Klenow large‐fragment domains Type 1 DNA polymerases from Thermus aquaticus (Klentaq) Escherichia coli (Klenow) under identical solution conditions in order to directly compare stabilization energetics two proteins. The high temperature stability Taq polymerase is common knowledge, basis its use chain reaction. This study, however, aimed at understanding thermodynamic for this high‐temperature stability. Chemical with...
Adipocyte lipid-binding protein (ALBP or aP2) is an intracellular fatty acid-binding that found in adipocytes and macrophages binds a large variety of lipids with high affinity. Although are frequently charged, biochemical studies proteins their interactions often focus most heavily on the hydrophobic aspects these interactions. In this study, we have characterized effects KCl stability lipid binding properties ALBP. We find added salt dramatically stabilizes ALBP, increasing its ΔG...
Polyubiquitination is a complex form of posttranslational modification responsible for the control numerous cellular processes. Many ubiquitin-binding proteins recognize distinct polyubiquitin chain types, and these associations help drive ubiquitin-signaling pathways. There considerable interest in understanding specificity proteins; however, because multivalent nature polyubiquitin, affinity measurements interactions that rely on affixing to surface can display artifactual,...
Sequence conservation analyses offer us a powerful glimpse of natural selection at work. Standard tools for measuring sequence report as function specific location in multiple alignment and have proven indispensable identifying highly constrained features such active site residues. The advent large-scale genomic sequencing efforts allows researchers to expand this paradigm investigate more nuanced relationships between function. Here, we present simple tool (SWiLoDD: Sliding Window Localized...