A5087992053- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- RNA Research and Splicing
- Protein Structure and Dynamics
- RNA Interference and Gene Delivery
- 14-3-3 protein interactions
- Microtubule and mitosis dynamics
- Nanopore and Nanochannel Transport Studies
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Lysosomal Storage Disorders Research
- International Arbitration and Investment Law
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- Dispute Resolution and Class Actions
- Force Microscopy Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Various Chemistry Research Topics
- Advancements in Semiconductor Devices and Circuit Design
- Air Traffic Management and Optimization
- Knowledge Management and Sharing
- Adhesion, Friction, and Surface Interactions
- Intellectual Property and Patents
- Advanced Electron Microscopy Techniques and Applications
Montclair State University
2021-2025
Yonsei University
2004-2021
University of Puget Sound
2019
Berkeley College
2012-2018
University of California, Berkeley
2011-2018
QB3
2011-2018
Korea Institute of Science & Technology Information
2002-2018
Howard Hughes Medical Institute
2011-2015
Korea Advanced Institute of Science and Technology
2012
University of Missouri
2011
Membrane scission by ESCRTs The ESCRT protein complexes are essential for cell division, the release of HIV from infected cells via budding, and other processes involving narrow membrane necks their inner surface. unusual inside-directed cutting has made it hard to recapitulate this reaction understand its mechanism. Schöneberg et al. encapsulated inside lipid vesicles used optical tweezers pull out nanotubes. In presence adenosine triphosphate, clusters generated force constricted nanotube,...
Significance The endosomal sorting complex required for transport (ESCRT) proteins remodel and sever membranes in a wide range of biology, including HIV budding. Electron microscopy has shown that ESCRT-III is often found contact with concave (negatively curved) membrane surfaces. It not been possible until now to determine whether the ESCRTs have an inherent preference binding because it difficult make nanoscale membranes. Here we made such on dimensions budding site. We show assembly...
Molecular diffusion at the surface of living cells is believed to be predominantly driven by thermal kicks. However, there growing evidence that certain cell molecules are fluctuating dynamics cortical cytoskeleton. Using fluorescence correlation spectroscopy, we measure coefficient a variety over temperature range 24-37 °C. Exogenously incorporated fluorescent lipids with short acyl chains exhibit expected increase this range. In contrast, find GPI-anchored proteins temperature-independent...
Cell plasma membranes are a heterogeneous mixture of lipids and membrane proteins. The importance lipid domains (also called rafts) as molecular sorting platform has been implicated in many physiological processes. that detached from the cytoskeletal structure spontaneously phase separate into distinct at equilibrium, which show their inherent demixing properties. Recently, researchers have discovered proteins with strong interprotein interactions also protein domains, thus enabling...
Lipid/cholesterol mixtures derived from cell membranes as well their synthetic reconstitutions exhibit well-defined miscibility phase transitions and critical phenomena near physiological temperatures. This suggests that lipid/cholesterol-mediated separation plays a role in the organization of live membranes. However, macroscopic lipid-phase is not generally observed membranes, degree to which properties isolated lipid are preserved membrane remain unknown. A fundamental property variation...
Ubiquitylation is involved in various physiological processes, such as signaling and vesicle trafficking, whereas ubiquitin (UB) considered an important clinical target. The polymeric addition of UB enables cargo molecules to be recognized specifically by multivalent binding interactions with UB-binding proteins, which results downstream processes. Recently, protein condensate formation ubiquitylated proteins has been reported many independent suggesting its potential role governing the...
HIV proteins exploit clathrin coats Clathrin-coated vesicles are involved in the sorting of membrane and cargo at trans-Golgi network. Clathrin adaptor proteins, including AP-1 AP-2, selecting their cargoes, assisted by small membrane-trafficking associated GTPase Arf1. Shen et al. were interested how HIV-1 Nef protein affected this process. They leveraged a structure solved presence to show that AP-1:clathrin coat is far more intricately organized than previously thought. Furthermore, Arf1...
This research seeks to answer the basic question, "How can we build up formula estimate proper royalty rate and up-front payment using data I get simply as input?" paper suggests a way derived from regression of historical dataset. analyzes dataset, including royalty-related like running (back-end payments) (up-front fee + milestones), regarding drug candidates for specific classes, anticancer or cardiovascular, by analysis. Then, predict is attrition corresponding development phase...
Protein cargos anchored on the lipid membrane can be segregated by fluidic domain phase separation. Lipid membranes at certain compositions may separate into domains to segregate cargos, and protein themselves involved in condensate formation with multivalent binding proteins cargos. Recent studies suggest that these two driving forces of separation closely interact promote codomain formation. In this report, we studied effect cargo density outcome giant unilamellar vesicles. Proteins lipids...
Fluorescence image analysis in biochemical science often involves the complex tasks of identifying samples for and calculating desired information from intensity traces. Analyzing giant unilamellar vesicles (GUVs) is one these tasks. Researchers need to identify many statistically analyze degree molecular interaction or state organization on membranes. This complicated, requiring a careful manual examination by researchers, so automating can significantly aid improving its efficiency...
Abstract The ESCRTs catalyze reverse-topology scission from the inner face of membrane necks in HIV budding, multivesicular endosome biogenesis, cytokinesis, and other pathways. We encapsulated a minimal ESCRT module consisting ESCRT-III subunits Snf7, Vps24, Vps2, AAA + ATPase Vps4 such that nanotubes reflecting correct topology could be pulled giant vesicles. Upon ATP release by photo-uncaging, this system was capable generating forces within manner dependent upon catalytic activity,...
Ubiquitylation is involved in various physiological processes our bodies such as signaling and vesicle trafficking, thus Ubiquitin (UB) an important medical target system of interest. Polymeric addition UB allows cargo molecules to be recognized specifically by multivalent binding interaction with proteins leading downstream processes. Recently, implication protein condensate formation Ubiquitylated have been reported many independent suggesting its potential role governing the spatial...