A5049646285- Photoreceptor and optogenetics research
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Biocrusts and Microbial Ecology
- Wastewater Treatment and Nitrogen Removal
- Mass Spectrometry Techniques and Applications
- Aquatic Ecosystems and Phytoplankton Dynamics
- RNA and protein synthesis mechanisms
- Photochromic and Fluorescence Chemistry
- Microbial Community Ecology and Physiology
Technische Universität Berlin
2019-2023
University of California, Davis
2017-2021
Colorado College
2010
Class III adenylyl cyclases generate the ubiquitous second messenger cAMP from ATP often in response to environmental or cellular cues. During evolution, soluble cyclase catalytic domains have been repeatedly juxtaposed with signal-input place synthesis under control of a wide variety these and endogenous signals. Adenylyl light-sensing proliferated photosynthetic species depending on light as an energy source, yet are also widespread nonphotosynthetic species. Among such naturally occurring...
Orthogonal translation is an efficient tool that provides many valuable spectral probes capable of covering different parts the electromagnetic spectrum and thus enabling parameterization various structural dynamic phenomena in proteins. In this context, nitrile-containing tryptophan analogs are very useful to study local electrostatics hydrogen bonding both rigid environments. Here, we report a semi-rational approach engineer tyrosyl-tRNA synthetase (TyrRS) variant Methanocaldococcus...
Cyanobacteriochromes (CBCRs) are photoreceptor proteins that photoconvert between two parent states and thereby regulate various biological processes. An intriguing property is their variable ultraviolet–visible (UV–vis) absorption covers the entire spectral range from far-red to near-UV region thus makes CBCRs promising candidates for optogenetic applications. Here, we have studied Slr1393, a CBCR photoswitches red- green-absorbing (Pr Pg, respectively). Using UV–vis absorption,...
Cyanobacteriochromes (CBCRs) are spectrally diverse photosensors from cyanobacteria distantly related to phytochromes that exploit photoisomerization of linear tetrapyrrole (bilin) chromophores regulate associated signaling output domains. Unlike phytochromes, a single CBCR domain is sufficient for photoperception. domains the production or degradation cyclic nucleotide second messengers becoming increasingly well characterized. Cyclic di-guanosine monophosphate (c-di-GMP) widespread...
Phytochromes and cyanobacteriochromes (CBCRs) use double-bond photoisomerization of their linear tetrapyrrole (bilin) chromophores within cGMP-specific phosphodiesterases/adenylyl cyclases/FhlA (GAF) domain-containing photosensory modules to regulate activity C-terminal output domains. CBCRs exhibit photocycles that are much more diverse than those phytochromes often found in large modular proteins such as Tlr0924 (SesA), one three blue light regulators cell aggregation the cyanobacterium...