A5066426380- Advanced MRI Techniques and Applications
- Bariatric Surgery and Outcomes
- RNA and protein synthesis mechanisms
- Medical Imaging Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Cardiac Imaging and Diagnostics
- Advanced X-ray and CT Imaging
- Gastric Cancer Management and Outcomes
- Radiation Dose and Imaging
- MRI in cancer diagnosis
- Colorectal and Anal Carcinomas
- Photoreceptor and optogenetics research
- Esophageal and GI Pathology
- Colorectal Cancer Surgical Treatments
- Chemical Synthesis and Analysis
- Sarcoma Diagnosis and Treatment
- Health and Medical Studies
- Lung Cancer Diagnosis and Treatment
- Experimental Behavioral Economics Studies
- Cardiac Arrhythmias and Treatments
- Anorectal Disease Treatments and Outcomes
- Cardiovascular Function and Risk Factors
- Immune Cell Function and Interaction
- Dermatological and COVID-19 studies
- Spectroscopy and Quantum Chemical Studies
Technical University of Munich
2020-2024
Technische Universität Berlin
2016-2023
University of Bayreuth
2023
Klinikum rechts der Isar
2020-2022
Smart Material (Germany)
2022
University Medical Center Freiburg
2006-2020
University of Freiburg
2008-2020
University of Wuppertal
2020
University College London
2018-2020
Hospital Clínic de Barcelona
2019
To evaluate traumatized bone marrow with a dual-energy (DE) computed tomographic (CT) virtual noncalcium technique.In this prospective institutional review board-approved study, 21 patients an acute knee trauma underwent DE CT and magnetic resonance (MR) imaging. A software application was used to virtually subtract calcium from the images. Presence of fractures noted, presence bruise rated on four-point scale for six femoral tibial regions by two radiologists. numbers were obtained in same...
Abstract Marine mussels exhibit potent underwater adhesion abilities under hostile conditions by employing 3,4‐dihydroxyphenylalanine (DOPA)‐rich mussel adhesive proteins (MAPs). However, their recombinant production is a major biotechnological challenge. Herein, novel strategy based on genetic code expansion has been developed engineering efficient aminoacyl‐transfer RNA synthetases (aaRSs) for the photocaged noncanonical amino acid ortho ‐nitrobenzyl DOPA (ONB‐DOPA). The engineered...
Allosteric information transfer in proteins has been linked to distinct vibrational energy (VET) pathways a number of theoretical studies. Experimental evidence for such pathways, however, is sparse because site-selective injection into protein, that is, localized heating, required their investigation. Here, we solved this problem by the site-specific incorporation non-canonical amino acid β-(1-azulenyl)-l-alanine (AzAla) through genetic code expansion. As an exception Kasha's rule, AzAla...
SIRT6 is a NAD+-dependent deacetylase that modulates chromatin structure and safeguards genomic stability. So far, has been assigned to the nucleus only nuclear targets of are known. Here, we demonstrate in response stress, C. elegans SIR-2.4 its mammalian orthologue localize cytoplasmic stress granules (SGs), interact with various SGs components induce their assembly. Loss or inhibition catalytic activity MEF cells impairs formation delays disassembly during recovery, while deficiency...
The incorporation of non-canonical amino acids (ncAA) is an elegant way for the chemical diversification recombinantly produced antimicrobial peptides (AMPs). Residue- and site-specific installation methods in several bacterial production hosts hold great promise generation new-to-nature AMPs, can contribute to tackle ongoing emergence antibiotic resistance pathogens. Especially from a pharmacological point view, desirable improvements span pH protease resistance, solubility, oral...
Engineering aminoacyl-tRNA synthetases (aaRSs) provides access to the ribosomal incorporation of noncanonical amino acids via genetic code expansion. Conventional targeted mutagenesis libraries with 5–7 positions randomized cover only marginal fractions vast sequence space formed by up 30 active site residues. This frequently results in selection weakly enzymes. To overcome this limitation, we use computational enzyme design generate a focused library aaRS variants. For redesign, photocaged...
Loss of anticancer NK cell function in AML patients is associated with fatal disease progression and remains poorly understood. Here, we demonstrate that AML-blasts isolated from rapidly inhibit escape cell-mediated killing. Transcriptome analysis cells exposed to revealed increased CREM expression transcriptional activity, indicating enhanced cAMP signalling, confirmed by uniform production the cAMP-inducing prostanoid PGE2 all AML-blast isolates patients. Phosphoproteome disclosed induced...
Liver sinusoidal endothelial cells (LSECs) are liver-resident antigen (cross)-presenting that generate memory CD8 T cells, but metabolic properties of LSECs and LSEC-primed remain understudied. Here, we report high-level mitochondrial respiration constitutive low-level glycolysis support LSEC scavenger sentinel functions. fail to increase co-stimulation after TLR4 activation, indicating absence functional maturation compared with immunogenic dendritic cells. show a transient burst oxidative...
Combined Assessment of Aortic Root Anatomy and Aortoiliac Vasculature With Dual-Source CT as a Screening Tool in Patients Evaluated for Transcatheter Valve ImplantationPhilipp Blanke1, Wulf Euringer1, Tobias Baumann1, Jochen Reinöhl2, Christian Schlensak3, Mathias Langer1 Gregor Pache1Audio Available | Share
To prospectively investigate the feasibility, image quality, and radiation dose for prospective electrocardiographically (ECG) triggered sequential dual-source computed tomographic (CT) angiography of thoracic aorta in comparison to retrospective ECG-gated helical CT angiography.This study was approved by institutional review board; informed consent obtained. One hundred thirty-nine patients referred ECG-assisted were enrolled. Inclusion criteria stable sinus rhythm heart rate 80 beats per...
According to recent model investigations, vocal tract resonance is relevant registers. However, no experimental corroboration of this claim has been published so far. In the present investigation, ten professional tenors’ configurations were analyzed using MRI volumetry. All subjects produced a sustained tone on pitch F4 (349 Hz) vowel /a/ (1) in modal and (2) falsetto register. The area functions estimated from data their associated formant frequencies calculated. second condition same...
Orthogonal translation is an efficient tool that provides many valuable spectral probes capable of covering different parts the electromagnetic spectrum and thus enabling parameterization various structural dynamic phenomena in proteins. In this context, nitrile-containing tryptophan analogs are very useful to study local electrostatics hydrogen bonding both rigid environments. Here, we report a semi-rational approach engineer tyrosyl-tRNA synthetase (TyrRS) variant Methanocaldococcus...
Abstract Macrophage-inflammatory protein 2 (MIP-2) is a major CXC chemokine involved in the migration of polymorphonuclear neutrophils (PMNs) to sites inflammation. Although cell culture experiments have identified different types that can produce MIP-2, cellular sources vivo are not clearly defined. By using immunohistochemical staining and analysis mRNA expression, present study aimed localize cells producing MIP-2 tissues normal mice challenged with Yersinia enterocolitica. The results...
Multislice whole body computed tomography is regarded as the method of choice for primary investigation hemodynamically stable patients with multiple injuries. However, a disadvantage this high level radiation to which patient exposed. Various recommendations on how position patient's arms during have been given in literature, but conclusive data are missing. Therefore, aim our study was investigate relationship between different arm positions and dose undergoing tomography.In retrospective...
Pancreatic islet cell tumors (ICTs) occur as sporadic neoplasias or a manifestation of multiple endocrine neoplasia type 1 (MEN1) and von Hippel–Lindau disease (VHL). Molecular classification ICTs is mandatory for timely diagnosis surveillance. Systematic comparison VHL-ICTs has been lacking. Our registry-based approaches used the German NET-Registry with 259 patients neuroendocrine (NETs), who were primarily diagnosed NETs, VHL-Registry 485 molecular genetically confirmed had undergone...