A5058644993- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- Electron Spin Resonance Studies
- Molecular Junctions and Nanostructures
- Light effects on plants
- Protein Structure and Dynamics
- Advanced Fluorescence Microscopy Techniques
- Advanced Thermodynamic Systems and Engines
- Microfluidic and Capillary Electrophoresis Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Various Chemistry Research Topics
- Mass Spectrometry Techniques and Applications
- Embedded Systems and FPGA Design
- Photosynthetic Processes and Mechanisms
- Chemical and Physical Studies
- Biotin and Related Studies
- Molecular spectroscopy and chirality
Goethe University Frankfurt
2018-2024
Allosteric information transfer in proteins has been linked to distinct vibrational energy (VET) pathways a number of theoretical studies. Experimental evidence for such pathways, however, is sparse because site-selective injection into protein, that is, localized heating, required their investigation. Here, we solved this problem by the site-specific incorporation non-canonical amino acid β-(1-azulenyl)-l-alanine (AzAla) through genetic code expansion. As an exception Kasha's rule, AzAla...
Abstract Vibrational energy transfer (VET) is essential for protein function. It responsible efficient dissipation in reaction sites, and has been linked to pathways of allosteric communication. While it understood that VET occurs via backbone as well non-covalent contacts, little known about the competition these two transport channels, which determines pathways. To tackle this problem, we equipped β-hairpin fold a tryptophan zipper with pairs non-canonical amino acids, one serving injector...
Abstract Vibrational energy transfer (VET) is emerging as key mechanism for protein functions, possibly playing an important role dissipation, allosteric regulation, and enzyme catalysis. A deep understanding of VET required to elucidate its in such processes. Ultrafast VIS‐pump/IR‐probe spectroscopy can detect pathways proteins. However, the requirement having a donor sensor installed simultaneously limits possible target proteins sites; increase their number we compare six IR labels...
Various protein functions are related to vibrational energy transfer (VET) as an important mechanism. The underlying pathways can be experimentally followed by ultrafast Vis-pump/IR-probe spectroscopy with a donor-sensor pair of non-canonical amino acids (ncAAs) incorporated in protein. However, so far only one donor ncAA, azulenylalanine (AzAla), exists, which suffers from comparably low Vis extinction coefficient. Here, we introduce two novel ncAAs based on iminothioindoxyl (ITI)...
Alternating acquisition of background and sample spectra is often employed in conventional Fourier-transform infrared spectroscopy or ultraviolet-visible for accurate subtraction. For example, solvent correction, typically a spectrum cuvette with measured subtracted from solute. Ultrafast spectroscopies, though, come many peculiarities that make the collection well-matched, subtractable challenging. Here, we present demountable split-sample cell combination modified Lissajous scanner to...
Abstract Eine Reihe theoretischer Studien setzt allosterischen Informationstransfer in Proteinen Beziehung zu definierten Schwingungsenergietransfer(VET)‐Pfaden. Experimentelle Evidenz für diese Pfade ist rar, da ihre Erforschung eine punktuelle Injektion von Schwingungsenergie Proteine, d. h. lokale Erhitzung, benötigt wird. Um Experimente ermöglichen, haben wir den ortsspezifischen Einbau der nichtkanonischen Aminosäure β‐(1‐Azulenyl)‐ l ‐alanin (AzAla) mithilfe des erweiterten genetischen...
Abstract Vibrational energy transfer (VET) is emerging as key mechanism for protein functions, possibly playing an important role dissipation, allosteric regulation, and enzyme catalysis. A deep understanding of VET required to elucidate its in such processes. Ultrafast VIS‐pump/IR‐probe spectroscopy can detect pathways proteins. However, the requirement having a donor sensor installed simultaneously limits possible target proteins sites; increase their number we compare six IR labels...
Many biological mechanisms rely on the precise control of conformational changes in proteins. Understanding such dynamic processes requires methods for determining structures and their temporal evolution. In this study, we introduce a novel approach to time-resolved ion mobility mass spectrometry. We validated method simple photoreceptor model applied it more complex system, animal-like cryptochrome from
Abstract Vibrational energy transfer (VET) is essential for protein function. It responsible the efficient dissipation of excess after enzymatic reactions and photochemical processes, has been linked to pathways allosteric signal transduction. While it understood that VET occurs via backbone as well non-covalent contacts, little known about competition these two transport channels, which determines VET. To tackle this problem, we equipped β-hairpin fold a tryptophan zipper with pairs...
Abstract Cryptochromes (CRYs) belong to the class of blue light photoreceptors and are responsible for various light-triggered functions in circadian rhythm upon excitation their inbuilt flavin cofactor. They functionally distinct evolutionarily related photolyases, which mediate light-driven repair UV induced DNA damages. Despite these functional differences they share a high degree structural sequence homology. A hallmark cryptochromes is flexible carboxyl-terminal extension (CTE), whose...
Abstract Various protein functions are related to vibrational energy transfer (VET) as an important mechanism. The underlying pathways can be experimentally followed by ultrafast Vis‐pump/IR‐probe spectroscopy with a donor‐sensor pair of non‐canonical amino acids (ncAAs) incorporated in protein. However, so far only one donor ncAA, azulenylalanine (AzAla), exists, which suffers from comparably low Vis extinction coefficient. Here, we introduce two novel ncAAs based on iminothioindoxyl (ITI)...
Schwingungsenergietransfer (VET) in Proteinen wird Computerstudien mit Allosterie und anderen Aspekten der Proteinfunktion Verbindung gebracht. J. Bredenbeck, N. Budisa Mitarbeiter zeigen ihrer Zuschrift auf S. 2925, wie Schwingungsenergie injiziert ortsaufgelöst experimentell nachverfolgt werden kann. Durch Verwendung eines genetisch codierten ultraschnellen Heizelements von Femtosekunden-IR-Spektroskopie ermöglichte dieser Ansatz die Kartierung VET-Pfaden Untersuchung Rolle des VET für...
Schwingungsenergietransfer in Proteinen wird mit der Katalyse von Enzymen, Energiedissipation und allosterischen Kommunikation Verbindung gebracht. In ihrem Forschungsartikel (e202200648) stellen Jens Bredenbeck Mitarbeiter eine breite Palette Schwingungsenergiesensoren vor. Eine Vielzahl Funktionalitäten deckt die chemische sterische Vielfalt ab. Ihre vergleichbare Responsivität ermöglicht es, für jede Position geeignete nicht-kanonische Aminosäure zu wählen, wie am Beispiel eines Proteins...