A5101713471- Neurotransmitter Receptor Influence on Behavior
- Neuroendocrine regulation and behavior
- Neuroscience and Neuropharmacology Research
- Tryptophan and brain disorders
- Receptor Mechanisms and Signaling
- Cannabis and Cannabinoid Research
- Stress Responses and Cortisol
- Chemical Reactions and Isotopes
- Obesity, Physical Activity, Diet
- MicroRNA in disease regulation
- Pharmacological Receptor Mechanisms and Effects
- Diet and metabolism studies
- Pharmacological Effects and Assays
- Circular RNAs in diseases
- Human Health and Disease
- Nicotinic Acetylcholine Receptors Study
- Pharmacogenetics and Drug Metabolism
- Cancer Treatment and Pharmacology
- Eicosanoids and Hypertension Pharmacology
- Cancer-related molecular mechanisms research
- Treatment of Major Depression
- Biochemical effects in animals
- Neuropeptides and Animal Physiology
- Smoking Behavior and Cessation
- Fatty Acid Research and Health
Masaryk University
1998-2021
Czech Academy of Sciences
1993-2019
Czech Academy of Sciences, Institute of Microbiology
2016-2019
Czech Technical University in Prague
2011-2012
Comenius University Bratislava
2006-2009
Public Health Authority of the Slovak Republic
2004-2009
Czech Academy of Sciences, Institute of Physiology
2005-2006
Physiol (Belgium)
2006
National Institutes of Health
1998
National Institute on Drug Abuse
1998
MicroRNAs are endogenously expressed regulatory noncoding RNAs. Previous studies showed altered expression levels of several microRNAs in glioblastomas. In this study, we examined the selected 22 primary glioblastomas and six specimens adult brain tissue by real-time PCR method. addition, methylation status MGMT promoter methylation-specific PCR, as has been shown to be a predictive marker was not correlated with response concomitant chemoradiotherapy temozolomide (RT/TMZ). MiR-221...
The aim of this study was to evaluate the effect intra-follicular treatment (IFT) with luteinizing hormone (LH) and human chorionic gonadotropin (hCG) in dairy heifers. Intra-follicular LH (2 µg, 0.2 ml, group), hCG (10 IU, group) or saline (0.2 S done into dominant follicle on seventh day a synchronized oestrous cycle. A response follicles subjected IFT D7 as well plasma progesterone concentration (D0) seven days later (D7) were evaluated. Ovulation development new corpus luteum occurred...
Cannabinoids are drugs that frequently abused not only alone, but also in combination with other drugs. The present study investigated possible functional interactions between the psychostimulant methamphetamine and cannabinoid receptor agonists anandamide, or R-(+)-methanandamide antagonist AM 251 rat model of i.v. drug self-administration. In rats trained to self-administer methamphetamine, intake was significantly decreased by tended be dose-dependently increased pre-treatment agonists....
Neurochemical studies indicate that methamphetamine increases central serotonin (5-HT) levels more markedly than other psychomotor stimulants such as amphetamine or cocaine. In the present study, we investigated 5-HT involvement in discriminative stimulus effects of methamphetamine. Sprague-Dawley rats trained to discriminate 1.0 mg/kg i.p. from saline under a fixed-ratio schedule food presentation, selected agonists, antagonists, and uptake inhibitors were tested. Fluoxetine (1.8-18.0...
Quambalarine B (QB) is a secondary metabolite produced by the basidiomycete Quambalaria cyanescens with potential anticancer activity. Here we report that QB at low micromolar concentration inhibits proliferation of several model leukemic cell lines (Jurkat, NALM6, and REH), whereas higher concentrations induce death. By contrast, effect on primary leukocytes (peripheral blood mononuclear cells) significantly milder lower toxicity cytostatic Moreover, inhibited expression C-MYC oncoprotein...
The discrete activation of individual caspases is essential during T-cell development, activation, and apoptosis. Humans carrying nonfunctional caspase-8 conditional knockout mice exhibit several defects in the progression naive CD4+ T cells to effector stage. MST1, a key kinase Hippo signaling pathway, often presented as substrate caspases, its cleavage by potentiates activity. Several studies have focused on involvement MST1 caspase also reported immune system function caused deficiency....
Abstract In proteomics, post-proline cleaving enzymes (PPCEs) like Aspergillus niger prolyl endopeptidase and neprosin complement proteolytic tools because proline is a stop site for many proteases. However, our systematic analysis of cleavage preferences showed that both PPCEs also display specificity to reduced cysteine. Post-cysteine was blocked by Cys alkylation, explaining why this activity has remained undetected. Our findings redefine their applicability how we study interpret mechanism.
In proteomics, postproline cleaving enzymes (PPCEs), such as Aspergillus niger prolyl endopeptidase (AnPEP) and neprosin, complement proteolytic tools because proline is a stop site for many proteases. But while aiming at using AnPEP in online proteolysis, we found that this enzyme also displayed specificity to reduced cysteine. By LC–MS/MS, systematically analyzed sources conditions could affect cleavage preference. Postcysteine was blocked by cysteine modifications, including disulfide...
Exchange of deuterium was studied with commercial potassium and barium hydrides, hydrides pure niobium tantalum metals, yttrium hydride, rare earths metals. Non-stoichiometric composition the made impossible a quantitative evaluation exchange mechanism. In most cases ability to hydride hydrogen found be connected thermal stability hydrides.