A5041023973- Microtubule and mitosis dynamics
- Reproductive Biology and Fertility
- Prenatal Screening and Diagnostics
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Cellular Mechanics and Interactions
- Plant Molecular Biology Research
- Modular Robots and Swarm Intelligence
- Assisted Reproductive Technology and Twin Pregnancy
- 3D Printing in Biomedical Research
- Ovarian function and disorders
- Evolutionary Algorithms and Applications
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
Institut Pasteur
2024
Biologie du Développement et Cellules Souches
2021
Centre Interdisciplinaire de Recherche en Biologie
2016-2020
Collège de France
2016-2020
Université Paris Sciences et Lettres
2016-2018
Inserm
2018
Centre National de la Recherche Scientifique
2018
Article12 January 2018Open Access Transparent process Shifting meiotic to mitotic spindle assembly in oocytes disrupts chromosome alignment Isma Bennabi Center for Interdisciplinary Research Biology (CIRB), College de France, CNRS, INSERM, PSL University, Equipe labellisée FRM, Paris, France Search more papers by this author Isabelle Quéguiner Agnieszka Kolano International Institute of Molecular and Cell Biology, Warsaw, Poland Thomas Boudier orcid.org/0000-0002-0148-7733 Université Pierre...
Human and mouse oocytes' developmental potential can be predicted by their mechanical properties. Their development into blastocysts requires a specific stiffness window. In this study, we combine live-cell computational imaging, laser ablation, biophysical measurements to investigate how deregulation of cortex tension in the oocyte contributes early failure. We focus on extra-soft cells, most common defect natural population. Using two independent tools artificially decrease cortical...
The mitotic spindle is an ensemble of microtubules responsible for the repartition chromosomal content between two daughter cells during division. In metazoans, assembly a gradual process involving dynamic and recruitment numerous associated proteins motors. During mitosis, centrosomes organize nucleate majority microtubules. contrast, oocytes lack canonical but are still able to form bipolar spindles, starting from initial ball that self-organizes in several hours. Interfering with early...
Understanding the interplay between cell fate specification and morphogenetic changes remains a central challenge in developmental biology. Gastruloids, self-organizing stem cell-based models of post-implantation mammalian development, provide powerful platform to address this question. Here, we show that physical parameters, particularly system size, critically influence timing outcomes processes. Larger gastruloids exhibit delayed symmetry breaking, increased multipolarity, prolonged axial...