A5075694253- Sperm and Testicular Function
- Ovarian function and disorders
- Hormonal and reproductive studies
- Reproductive Biology and Fertility
- Selenium in Biological Systems
- Trace Elements in Health
- Cholesterol and Lipid Metabolism
- Sexual Differentiation and Disorders
- melanin and skin pigmentation
- Rabbits: Nutrition, Reproduction, Health
- Caveolin-1 and cellular processes
- Lipoproteins and Cardiovascular Health
- Antioxidant Activity and Oxidative Stress
- Renal and related cancers
- Bee Products Chemical Analysis
- Connexins and lens biology
- Reproductive biology and impacts on aquatic species
- Pesticide Exposure and Toxicity
- Stress Responses and Cortisol
- Urological Disorders and Treatments
- Sulfur Compounds in Biology
- Proteins in Food Systems
- Adipose Tissue and Metabolism
Instituto de Histología y Embriología de Mendoza
2010-2021
Consejo Nacional de Investigaciones Científicas y Técnicas
2010-2021
Universidad Nacional de Cuyo
2007-2021
Aconcagua University
2011-2019
Hypercholesterolemia represents a high risk factor for frequent diseases and it has also been associated with poor semen quality that may lead to male infertility. The aim of this study was analyze sperm function in diet-induced hypercholesterolemic rabbits. Twelve adult White New Zealand rabbits were fed ad libitum control diet or supplemented 0.05% cholesterol. Rabbits under cholesterol-enriched significantly increased total cholesterol level the serum. Semen examination revealed...
Fat increment (0.05% cholesterol, chol) in standard diet promoted a significant increase serum and sperm membrane chol, which ultimately altered membrane-coupled specific functions: osmotic resistance, acrosomal reaction, capacitation White New Zealand rabbits. These changes were also associated with reduction motility percentage appearance of abnormal morphology. The present study was carried out to evaluate the effect dietary olive oil (OO, 7% v/w) administration several male...
Abstract Mammalian sperm proteins undergo thiol group (SH) oxidation to form disulfides bonds (SS) as they travel through the epididymis during cell maturation. Disulfide are involved in chromatin condensation and tail organelle stabilization. In this work, we used a fluorescent thiol‐selective labeling agent, monobromobimane (mBBr), study protein status of rat Fluorescence signal decrease along epididymal trip, more evidently head, but also tail, indicates that both sub cellular regions...
Peroxynitrite is a highly reactive nitrogen species and potent inducer of apoptosis necrosis in somatic cells. Peroxynitrite-induced nitrosative stress has emerged as major cause impaired sperm function; however, its ability to trigger cell death not been described human spermatozoa. The objective here was characterize biochemical morphological features induced by peroxynitrite-mediated For this, spermatozoa were incubated with without (untreated control) 3-morpholinosydnonimine (SIN-1),...
Hypercholesterolemia is a marker for several adult chronic diseases. Recently we demonstrated that sub/infertility also associated to in rabbits. Seminal alterations included: abnormal sperm morphology, decreased number and declined percentage of motile sperm, among others. In this work, our objective was evaluate the effects hypercholesterolemia on testicular efficiency spermiogenesis, as latter are directly related morphology respectively. Tubular determined by comparing total...
Peroxynitrite is a highly reactive nitrogen species and when it generated at high levels causes nitrosative stress, an important cause of impaired sperm function. High peroxynitrite have been shown to correlate with decreased semen quality in infertile men. Thiol groups are mainly found enzymes, antioxidant molecules, structural proteins the axoneme. primarily reacts thiol cysteine-containing proteins. Although well known that oxidizes sulfhydryl sperm, subcellular localization this...
Male infertility is a disorder of the reproductive system defined by failure to achieve clinical pregnancy after 12 months or more regular unprotected sexual intercourse. The presence low-motile immotile spermatozoa one many causes infertility; however, this observation provides little no information regarding pathogenesis malfunction. Good sperm motility depends on correct assembly tail in testis and efficient maturation during epididymal transit. Thiols flagellar proteins, such as outer...
A chronic-positive energetic balance has been directly correlated with infertility in men, but the involved mechanisms remain unknown. Herein we investigated weather a mouse model chronic feeding diet supplemented chicken fat affects sperm head morphology. To accomplish this, fed mice for 16 weeks either control food (low-fat diet, LFD) or 22% (high-fat HFD). At end of regimen, measured: redox and inflammatory changes, cholesterol accumulation testis analyzed testicular morphological...
Abstract In many mammalian species, sperm associate as a consequence of the epididymal transit. From classic Rouleaux in guinea pig to most recent work mouse and echidna, authors have focused mainly on detailed morphological description this phenomenon. Some these articles also begun describe nature material present between heads. Here, we try better understand factor/s involved rat association (Rosette). Based previous describing appearance Rosettes distal segments epididymis, consider that...
High-fat diet is associated with hypercholesterolemia and seminal alterations in White New Zealand rabbits. We have previously reported disorders the development of manchette-acrosome complex during spermiogenesis decreased testicular efficiency hypercholesterolemic On other hand, olive oil incorporated into improves cholesterolemia semen parameters affected In this paper, we report recovery—with addition to diet—from sub-cellular mechanisms involved shaping sperm cell altered Using...
Abstract In many mammals, sperm associations had been observed, but not in the mouse. this work, mouse rosettes are morphologically described inside epididymis and during its dissolution a culture medium. Also characterized saccharides present linking material. Sperm association other epididymal actions supported by transit verified at caudal region, suggesting relation between maturation. drops of content obtained from distal (cauda), proximal (caput corpus) regions; dissolved medium,...
Male fertility has been shown to be dependent on cholesterol homeostasis. This lipid is essential for testosterone synthesis and spermatogenesis, but its levels must maintained in an optimal range proper testicular function. In particular, sperm cells' development very sensitive high levels, noticeably during acrosomal formation. The aim of this work was study whether the molecular pathway that regulates intracellular cholesterol, sterol regulatory element-binding protein (SREBP) pathway,...
ODF1 has been described as an exclusively expressed testicular protein and is located in the outer dense fibers along sperm tail. involved motility development of flagellum, but function not already clear. Other ODF proteins, such ODF2 have characterized other tissues like basal body kidney primary cilium, so far only mRNA tissues. These observations let us to hypothesize that expression could be limited testis. Therefore, present work we proposed evaluate if also than Here demonstrated...