During the investigation of an outbreak infection caused by Proteus mirabilis in infants' nursery it was found that solu tions disinfectants use there were contaminated with viable Gram-negative bacilli. The organisms cultured not sp., and quite unconnected outbreak. This finding prompted us to examine stocks hospital for presence living bacteria. Subsequent showed aqueous solutions Hibitane (chlorhexidine) Savlon (cetrimide-chlorhexidine mixture ratio 10:1) issued from pharmacy throughout...

A study was made of the origin, occurrence, and properties natural antibodies to Gram-negative bacteria in normal serum several species. Antibody measured by a procedure based on bactericidal reaction carried out under conditions which activity function amount antibody contributed test serum. seven genera family Enterobacteriaceae were demonstrated human The specificity these affirmed absorption with homologous inhibition purified somatic antigen. Absorption graded amounts bacterial...

Landy, Maurice (National Cancer Institute, Bethesda, Md.), J. Gabriel Michael, and James L. Whitby . Bactericidal method for the measurement in normal serum of antibody to gram-negative bacteria. Bacteriol. 83: 631–640. 1962.—The bactericidal effect on bacteria was investigated with objective determining whether this antibody-complement reaction could be used antibody. The major variables affecting outcome complement-dependent were explored systematically establish test conditions which...

The hydraulic conductivity of natural clays in the 1.5 m contamination zone below a 15 year old domestic waste landfill has been determined.Water-soluble contaminants such as chloride, sodium, and dissolved organic carbon have migrated about 1.0 compared with only cm for copper, zinc, iron, lead, manganese. migration, primarily by diffusion, rendered clay perfect assessment clay–leachate compatibility respect to conductivity, k.Oedometer tests on tube samples yielded k values 1.4 × 10 −8...

SummaryGerm-free and conventionally reared mice of similar genetic stock maintained on the same sterilized diet were compared as to their reaction bacterial endotoxin. No significant difference was found in following: elevation levels antibody Gram-negative bacteria, stimulation glycolytic activity spleen cells peritoneal macrophages, susceptibility infection with Salmonella typhosa, production by endotoxin increased resistance infection, LD50

SUMMARY A series of 330 consecutive specimens urine is reported and the frequency isolation various infecting organisms recorded. One‐third all infected urines received were regarded as growing contaminants rather than true pathogens. larger Gram‐negative isolated from also analysed. Esch. coll was found to be most frequent urinary pathogen isolated. The incidence described atypical coliforms discussed freundii in 12–8 per cent, infections. K. aerogenes infection only 2 cent.

The experimental procedure described is designed to allow calculation of the radiation sterilization dose for medical devices any desired standard sterility assurance. makes use results a series tests on device samples exposed doses from 0.2 1.8 Mrad in increments. From test data 10(-2) level determined. A formula that allows value called DS be calculated. This an estimate effective resistance heterogeneous microbial population remaining tail portion inactivation curve at and above. used as...

Water containing ornamental fishes was found to frequently contain countable numbers of bacteria that were resistant one or more antibiotic chemotherapeutic agents. The multidrug-resistant strains most commonly isolated lactose-fermenting Citrobacter freundii . overall resistance these aquaria greater than the previously described clinical isolates C. Although examined appeared lack R-factors, this pool may have public health implications.

SUMMARY A method for pyocin-sensitivity typing by means of “phage-free” preparations pyocin is described. The was tested on 227 isolates P. aeruginosa, collected from 34 different foci infection in hospitals the British Isles and results were compared with those combined serological phage all strains production 105 isolates. It concluded that a simple reliable giving high degree discrimination, comparable to typing, it suitable use routine hospital laboratories.

The nature of bacteriological investigations creates data processing problems. Non-numerical results must be coded. This may done retrospectively or at the bench, former method involving extra work and staff, latter implying simple codes, stringent checking, a nearby computer, decoding programs. use shared computer for producing daily reports diminishes departmental autonomy. Benefits occur in more readily available records statistics, quality control, epidemiology, diagnostic bacteriology.