A5091542363- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Regulation of Appetite and Obesity
- Advanced Chemical Sensor Technologies
- Neuroscience and Neuropharmacology Research
- Diet and metabolism studies
- Bariatric Surgery and Outcomes
- Receptor Mechanisms and Signaling
- Neuroendocrine regulation and behavior
- Memory and Neural Mechanisms
- Nutritional Studies and Diet
- Neurobiology and Insect Physiology Research
- Diabetes Treatment and Management
- Biochemical effects in animals
- Eating Disorders and Behaviors
- Obesity, Physical Activity, Diet
- Sensory Analysis and Statistical Methods
- Adipose Tissue and Metabolism
- Animal Nutrition and Physiology
- Neuropeptides and Animal Physiology
- Neurotransmitter Receptor Influence on Behavior
- Obesity and Health Practices
- Ion Transport and Channel Regulation
- Plant-based Medicinal Research
- Diet, Metabolism, and Disease
Florida State University
2015-2024
University of Florida
2003-2020
University of Southern California
2018
Taste and Smell Clinic
2002-2012
University of Maryland, Baltimore
2007
Pennsylvania State University
1994
University of Pennsylvania
1988-1993
Monell Chemical Senses Center
1993
Hebrew University of Jerusalem
1979-1980
Roux-en-Y gastric bypass is the most effective therapy for morbid obesity. This study investigated how affects intake of and preference high-fat food in an experimental (rat) within a trial setting (human). Proportion dietary fat patients was significantly lower 6 yr after surgery compared with vertical-banded gastroplasty ( P = 0.046). Gastric reduced total caloric < 0.001) increased standard low-fat chow consumption sham controls rats. Compared sham-operated rats, rats displayed much...
A microstructural analysis of licking behavior in nondeprived and 23-hr food-deprived rats (n = 15) presented with various sucrose solutions (0.03-1.0 M) daily single-bottle, 1-hr sessions was conducted. Food deprivation concentration interacted to increase total licks. The effects food on burst size (BS), number (BN), other parameters varied as a function the pause criterion (PC; 0.3-100 s) used define bursts. rationale for selecting 1-s PC further is presented. Despite lack correlations...
Amiloride, an epithelial sodium channel blocker, suppresses the responsiveness of narrowly tuned sodium-responsive taste afferents when orally applied in rat. Broadly salt-responsive afferents, which respond to and nonsodium salts acids, are relatively unaffected by drug. We used amiloride treatment examine consequences specific removal input from on discrimination. Five water-restricted rats were trained a gustometer press one lever after licking NaCl another KCl across range concentrations...
Lesions in the gustatory zone of parabrachial nuclei (PBN) severely impair acquisition a conditioned taste aversion (CTA) rats. To test whether this deficit has memorial basis, intact rats (n = 15) and with PBN lesions (PBNX; n 10) received seven intraoral stimulus infusions (30 s, 0.5 ml) distributed over 30.5-min period after either LiCl or NaCl injection. This task measures rapid formation CTA minimum demands on memory. LiCl-injected progressively changed their oromotor response profile...
Several explanations may account for deficits in the ability of animals to form taste aversions following neural manipulations. These encompass impairments conditioned stimulus (CS) and unconditioned (US) processing, response (CR) measurement, expression, memory, taste-visceral integration. A behavioral procedure that aids distinction between some these possibilities is presented. In Experiment 1, 10 rats received seven intraoral (IO) infusions sucrose (30 s, 0.55 ml) spaced every 5 min...
The T1R2 and T1R3 proteins are expressed in taste receptor cells form a heterodimer binding with compounds described as sweet by humans. We examined whether Polycose might be mediated through this testing knockout (KO) KO mice their wild-type (WT) littermate controls series of brief-access tests (25-min sessions 5-s trials). Sucrose, Na-saccharin, were each tested for three consecutive order presentation varied among subgroups Latin-Square manner. Both groups displayed blunted licking...
Gustatory deafferentation of the anterior tongue by bilateral section chorda tympani nerve, which removes only 15% total taste buds in rat, severely impaired rat's ability to discriminate NaCl from KCl. The discrimination deficit was selective. Denervated rats were able sucrose quinine. Despite eliminating four times as many glossopharyngeal posterior lingual had no effect on vs. KCl performance. Collectively, these data suggest that afferents nerve provide highest degree disparity between...
The rat glossopharyngeal nerve (GL), which innervates posterior tongue taste buds, contains several physiologically defined fiber types; at least one type is primarily responsive to certain alkaloids (such as quinine), and another acids salts. In contrast, the chorda tympani (CT), anterior does not appear contain fibers that differentially respond quinine relative salts acids. It was therefore predicted GL transection should disrupt behavioral discriminations between either or...
Alterations in the motivation to ingest sucrose can be quantified by measuring number and type of oral motor somatic responses (i.e., taste reactivity [TR]) that are elicited sucrose. In 2 experiments, rats had intraorally infused paired with LiCl injections for several trials, or they were injected every 5 min during 30-min postinjection period (data from Spector, Breslin, & Grill, 1988). both ingestive TR decreased, whereas aversive increased over trials. Individual response components...
We examined the necessity of α-gustducin, a G protein α-subunit expressed in taste cells, to taste-mediated licking responses mice sapid stimuli. To this end, we measured α-gustducin knock-out (Gus−/−) and heterozygotic littermate controls (Gus+/−) variety 'bitter', 'umami', 'sweet', 'salty' 'sour' All previous studies how Gus−/− ingest stimuli have used long-term (i.e. 48 h) preference tests, which may be confounded by post-ingestive and/or experiential effects minimized these confounds...
Current evidence suggests salt taste transduction involves at least two mechanisms, one that is amiloride sensitive and appears to use apically located epithelial sodium channels relatively selective for Na + a second insensitive uses variant of the transient receptor potential vanilloid 1 (TRPV1) serves as nonspecific cation channel. To provide functional context these findings, we trained Trpv1 knockout (KO) wild-type (WT) C57BL/6J mice ( n = 9 or 10/group) in two-response operant...